Construct: ORF TRCN0000475532

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011594.1_s317c1
Derived from:
ccsbBroadEn_04869
DNA Barcode:
ATTCTAGTCAACACACTACTTGGT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CPNE4 (131034)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475532
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 131034 CPNE4 copine 4 NM_130808.2 100% 100%
2 human 131034 CPNE4 copine 4 XM_024453339.1 100% 100%
3 human 131034 CPNE4 copine 4 NM_001289112.1 96.8% 96.8% 1_54del
4 human 131034 CPNE4 copine 4 NM_153429.1 96.8% 96.8% 1_54del
5 human 131034 CPNE4 copine 4 XM_024453338.1 96.2% 96.2% 1_66del
6 human 131034 CPNE4 copine 4 XM_011512406.2 79.7% 79.7% 0_1ins339
7 human 131034 CPNE4 copine 4 XM_011512407.2 79.7% 79.7% 0_1ins339
8 human 131034 CPNE4 copine 4 XM_011512408.2 79.7% 79.7% 0_1ins339
9 human 131034 CPNE4 copine 4 XM_024453340.1 79.7% 79.7% 0_1ins339
10 human 131034 CPNE4 copine 4 XM_017005694.2 74.9% 74.9% 1_66del;1368_1369ins369
11 human 131034 CPNE4 copine 4 XM_017005695.2 68.9% 55.1% (many diffs)
12 human 131034 CPNE4 copine 4 XM_017005696.1 54.9% 54.9% 0_1ins753
13 mouse 74020 Cpne4 copine IV NM_028719.1 91.2% 99.4% (many diffs)
14 mouse 74020 Cpne4 copine IV XM_006511840.3 88.4% 96.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1737
ORF length:
1671
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgaa gaagatgagc aacatttatg agtccgctgc caacacactg ggaatcttta
121acagcccctg cctgaccaaa gttgagctgc gtgtggcgtg caaaggcatt tctgacagag
181atgccctttc caaaccagac ccctgtgtca tcctcaagat gcagtctcat gggcagtggt
241ttgaggttga caggactgag gtgattcgca cctgcataaa cccagtgtac tcaaaactgt
301ttactgtgga cttttacttt gaggaggtgc agcgcctgcg gtttgaagtc catgacatca
361gcagcaacca caatgggctg aaggaggccg acttccttgg tggcatggag tgcacacttg
421gccagattgt ttcccagaga aagctgtcca aatccttgct gaagcatggg aacacagcag
481ggaaatcttc catcacggtg attgctgaag aattatctgg caatgacgac tatgttgagc
541ttgcattcaa tgcacggaaa ttggatgaca aggatttctt cagtaaatct gacccatttc
601tggaaatttt tcgtatgaat gatgatgcaa ctcagcagct ggtgcaccga actgaggttg
661tgatgaataa cttaagccca gcctggaaat cattcaaagt atctgtaaat tctctatgca
721gcggagaccc agaccgccgg ctaaagtgca tagtatggga ctgggactcc aatggcaagc
781atgacttcat tggagaattc acctcgacat tcaaggagat gagaggagca atggaaggga
841aacaggtgca gtgggagtgc atcaatccca agtacaaagc caagaagaag aattacaaga
901actcaggcac tgtgattctg aatctgtgca agattcacaa gatgcattct ttcttggact
961acatcatggg tggctgccaa atccagttta cagtagctat agatttcact gcctcaaacg
1021gggaccccag gaacagctgt tccttgcact acatccaccc ttaccaaccc aatgagtatc
1081tgaaagcttt ggtagctgtg ggggagattt gccaagacta tgacagtgac aaaatgttcc
1141ctgcctttgg gtttggcgcc aggatacctc cagagtacac ggtctctcat gactttgcaa
1201tcaactttaa tgaagacaac ccagaatgtg caggaattca aggagttgtg gaagcctatc
1261agagctgtct tcctaagctc caactctacg gtcccaccaa cattgccccc atcatccaga
1321aggttgccaa gtcagcgtca gaggaaacta acaccaagga ggcatcgcaa tacttcatcc
1381tgctgatcct gacagatggt gttatcacag acatggccga cacccgggag gccattgtcc
1441atgcctccca cctccccatg tcagtcatca tcgtgggagt agggaacgct gacttcagtg
1501acatgcagat gctggacggt gatgatggga tTCTGAGGTC ACCCAAGGGA GAGCCTGTTC
1561TTCGAGACAT CGTCCAGTTC GTGCCCTTCA GGAACTTCAA ACACGCATCT CCAGCTGCCC
1621TGGCAAAGAG CGTGCTGGCT GAAGTCCCAA ACCAAGTTGT GGACTATTAC AATGGCAAAG
1681GAATTAAACC AAAATGTTCA TCAGAAATGT ATGAATCTTC CAGAACACTA GCACCATGCC
1741CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC
1801TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT
1861ATATATCTTG TGGAAAGGAC GAATTCTAGT CAACACACTA CTTGGTACGC GTTAAGTCga
1921caatcaacct ctggattaca aaatttgtga aagatt

Download FASTA (ORF) (Full)