Construct: ORF TRCN0000475533
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014495.1_s317c1
- Derived from:
- ccsbBroadEn_15274
- DNA Barcode:
- GGCCTGTGTCTGACCATTGCGCAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- HFM1 (164045)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475533
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 164045 | HFM1 | helicase for meiosis 1 | XM_024453708.1 | 47.8% | 7.1% | (many diffs) |
| 2 | human | 164045 | HFM1 | helicase for meiosis 1 | XM_017000495.1 | 47.3% | 7.1% | (many diffs) |
| 3 | human | 164045 | HFM1 | helicase for meiosis 1 | XM_017000496.1 | 47.3% | 7.1% | (many diffs) |
| 4 | human | 164045 | HFM1 | helicase for meiosis 1 | XM_017000494.1 | 44.1% | 6.6% | (many diffs) |
| 5 | human | 164045 | HFM1 | helicase for meiosis 1 | XM_017000491.1 | 27.7% | 4.1% | (many diffs) |
| 6 | human | 164045 | HFM1 | helicase for meiosis 1 | XM_017000490.1 | 27.4% | 4.1% | (many diffs) |
| 7 | human | 164045 | HFM1 | helicase for meiosis 1 | NM_001017975.5 | 26.9% | 4% | (many diffs) |
| 8 | human | 164045 | HFM1 | helicase for meiosis 1 | XM_011540849.1 | 26.9% | 4% | (many diffs) |
| 9 | human | 164045 | HFM1 | helicase for meiosis 1 | XM_011540850.2 | 26.9% | 4% | (many diffs) |
| 10 | human | 164045 | HFM1 | helicase for meiosis 1 | XM_011540851.1 | 26.9% | 4% | (many diffs) |
| 11 | human | 164045 | HFM1 | helicase for meiosis 1 | XM_011540852.2 | 26.9% | 4% | (many diffs) |
| 12 | human | 164045 | HFM1 | helicase for meiosis 1 | XR_001737010.1 | 25.4% | (many diffs) | |
| 13 | human | 164045 | HFM1 | helicase for meiosis 1 | XR_001737009.1 | 24.6% | (many diffs) | |
| 14 | human | 164045 | HFM1 | helicase for meiosis 1 | XR_001737008.1 | 24.2% | (many diffs) | |
| 15 | human | 164045 | HFM1 | helicase for meiosis 1 | XM_011540855.2 | 24.1% | 1.5% | (many diffs) |
| 16 | human | 164045 | HFM1 | helicase for meiosis 1 | XM_011540857.1 | 17.2% | 2.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 249
- ORF length:
- 180
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctgaaatca aatgattgcc tgttttcttt ggaaaatttg ttttttgaaa 121 aaccagatga agttgaaaag cagccagaca atgaaaagtc attggattgg tttctccctc 181 ctgctccatt gatttcagaa attccagata ctcaggagtt agaggaagaa ttagaaagtc 241 ataaactgta aagtcaggaa aagaggccaa aaatgttaac atcaaattta aagataacta 301 atgaagatac aaattatatt tcactaacat aaaaattcca gtttgccttt ccttctgata 361 aatatgaaca ggatgatcta aatttagaag gggtaggtaa taatgactta ccacatgttg 421 ctggcaagct gacatatgct tctcagaaat ataaaaatca cattggcact gagatagcac 481 ctgagaagag tgttcctgat gatacaaaat tagttaattt tgcagaagat aaaggagaga 541 gcacatcagt attccggaaa agattattta aaatatctga caatatacat gggagtgctt 601 attctaatga caatgaattg gactctcaca ttggctcagt gaaaattgta caaacagaaa 661 tgaacaaagg gaaatcaagg aactatagca atagtaagca gaaatttcag tattctgcaa 721 atgtgtttac agcaaataat gctttttctg cttctgaaat cggagaaggc atgttcaaag 781 caccatcttt ttcagttgct ttccaacctc atgatattca agaggtaaca gaaaatggtt 841 taggttcctt gaaggctgtc acagaaattc cggcaaaatt tagaagtatt ttcaaagaat 901 ttccatattt caactatata cagtccaagg cctttgatga tcttctttac acagatagga 961 attttgtgat ttgtgctcca actggttctg gaaaaactgt agtgtttgaa ctagctataa 1021 caagattgtt aatggaagta ccattgccat ggttgaatat taaaattgtt tacatggcac 1081 caataaaagc cttgtgcagt cagcgttttg atgactggaa agaaaaattt ggaccaatag 1141 gattgaattg taaagaactt actggagata cagtaatgga tgatctattt gagattcagc 1201 atgcccatat tattatgaca actccagtag gaaAAATGGG ATTGCCAACT TTCTTGTACA 1261 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1321 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1381 AGGACGAGGC CTGTGTCTGA CCATTGCGCA AACGCGTTAA GTCgacaatc aacctctgga 1441 ttacaaaatt tgtgaaagat t