Construct: ORF TRCN0000475534
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006173.1_s317c1
- Derived from:
- ccsbBroadEn_07206
- DNA Barcode:
- TTTGGTTTATTTCAGTCCTTCAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC14A2 (8170)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475534
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8170 | SLC14A2 | solute carrier family 14 me... | NM_001242692.2 | 99.8% | 99.6% | (many diffs) |
| 2 | human | 8170 | SLC14A2 | solute carrier family 14 me... | NM_001371319.1 | 99.8% | 99.6% | (many diffs) |
| 3 | human | 8170 | SLC14A2 | solute carrier family 14 me... | NM_007163.4 | 99.8% | 99.6% | (many diffs) |
| 4 | human | 8170 | SLC14A2 | solute carrier family 14 me... | XM_024451270.1 | 99.8% | 99.6% | (many diffs) |
| 5 | human | 8170 | SLC14A2 | solute carrier family 14 me... | XM_024451271.1 | 99.8% | 99.6% | (many diffs) |
| 6 | human | 8170 | SLC14A2 | solute carrier family 14 me... | XM_017026016.2 | 94.9% | 94.7% | (many diffs) |
| 7 | mouse | 27411 | Slc14a2 | solute carrier family 14 (u... | NM_207651.3 | 84.4% | 84.5% | (many diffs) |
| 8 | mouse | 27411 | Slc14a2 | solute carrier family 14 (u... | NM_001110273.1 | 37.6% | 39.1% | (many diffs) |
| 9 | mouse | 27411 | Slc14a2 | solute carrier family 14 (u... | NM_001110274.1 | 30.1% | 30.1% | (many diffs) |
| 10 | mouse | 27411 | Slc14a2 | solute carrier family 14 (u... | XM_017317915.1 | 28.7% | 25.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2829
- ORF length:
- 2760
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtctgacccc cacagcagtc ctctcctgcc agagccactt tccagcagat 121 acaaactcta cgaggcagag tttaccagcc cgagctggcc ctcgacatcc ccggatactc 181 acccagctct gcccctcctg gaaatgcctg aagaaaagga tctccggtct tccaatgaag 241 acagtcacat tgtgaagatc gaaaagctca atgaaaggag taaaaggaaa gacgacgggg 301 tggcccatcg ggactcagca ggccaaaggt gcatctgcct ctccaaagca gtgggctacc 361 tcacgggcga catgaaggag tacaggatct ggctgaaaga caagcacctt gccctccagt 421 tcatagactg ggtcctgaga gggaccgctc aggtgatgtt cgtcaacaat cctctcagcg 481 gcctcatcat cttcataggg ctgctgatcc agaatccctg gtggacaatc actgggggcc 541 tggggacagt ggtctcgacc ttaacagctc tcgccttggg ccaagacagg tctgccattg 601 cctcaggact ccatgggtac aacgggatgc tggtgggact gctgatggcc gtgttctcgg 661 agaagttaga ctactactgg tggcttctgt ttcctgtgac cttcacagcc atgtcctgcc 721 cagttctttc tagtgccttg aattccatct tcagcaagtg ggacctcccg gtcttcactc 781 tgcccttcaa cattgcagtc accttgtacc ttgcagccac aggccactac aacctcttct 841 tccccacaac actggtagag cctgtgtctt cagtgcccaa tatcacctgg acagagatgg 901 aaatgcccct gctgttacaa gccatccctg ttggggtcgg ccaggtgtat ggctgtgaca 961 atccctggac aggcggcgtg ttcctggtgg ctctgttcat ctcctcgcca ctcatctgct 1021 tgcatgcagc cattggctca atcgtggggc tgctagcagc cctgtcagtg gccacaccct 1081 tcgagaccat ctacacaggc ctctggagct acaactgcgt cctctcctgc atcgccatcg 1141 gaggcatgtt ctatgccctc acctggcaga ctcacctgct ggccctcatc tgtgccctgt 1201 tctgtgcata catggaagca gccatctcca acatcatgtc agtggtgggc gtgccaccag 1261 gcacctgggc cttctgcctt gccaccatca tcttcctgct cctgacgaca aacaacccag 1321 ccatcttcag actcccactc agcaaagtca cctaccccga ggccaaccgc atctactacc 1381 tgacagtgaa aagcggtgaa gaagagaagg cccccagcgg aggcggtggg gagcatccac 1441 ccacagcagg cccaaaggtg gaggagggct cggaggctgt gctctccaag cacaggagtg 1501 tatttcacat cgagtggtca tccattcgga ggaggagcaa agtgtttgga aaaggcgaac 1561 accaggaaag acaaaacaaa gacccatttc cctatcaata ccggaagccc acagtcgagc 1621 tgcttgatct ggacaccatg gaggagagct ctgagataaa agtggaaaca aacatttcca 1681 agacatcctg gattcggagt tccatggctg ccagtgggaa aagggtgagc aaagccctca 1741 gctacatcac aggagagatg aaggagtgtg gagagggact taaagacaag tccccagtgt 1801 tccagttctt tgactgggtc ctccgaggca catctcaagt gatgtttgtg aacaaccccc 1861 tcagcggcat cctcatcatc ctcggcctct tcatccagaa cccctggtgg gcgatctcag 1921 gctgcctggg taccatcatg tccaccttga cagccctcat cctgagtcag gacaagtcgg 1981 ccatcgctgc aggatttcac ggctacaatg gggtgctggt ggggctgctg atggccgtgt 2041 tctcagacaa aggtgactac tactggtggc tgttgctacc cgtcatcatc atgtccatgt 2101 cttgccccat cctctccagt gccctgggta ccatcttcag caagtgggac ctcccagtct 2161 tcacactgcc cttcaatatc actgtgactt tgtacctggc agccacgggc cactacaacc 2221 ttttcttccc cacaacgctg ctgcagcctg catccgccat gcccaacatc acctggtcag 2281 aggtccaagt gcccttgctt ttgagagcca tccccgttgg aattggccaa gtgtacggct 2341 gtgataaccc ctggactgga ggcatcttcc tcatagctct gttcatatcc tcacctctca 2401 tttgcttgca tgcagcaatt ggatccacca tggggatgct agcagcactc actattgcga 2461 cgccctttga ctccatctac ttcggcctgt gtggcttcaa cagcaccctc gcatgcatag 2521 cgataggagg catgttctac gtcatcacct ggcagacgca cctCCTCGCC ATCGCCTGCG 2581 CACTGTTTGC TGCCTACCTG GGTGCTGCCC TGGCTAACAT GTTATCTGTG TTTGGATTGC 2641 CGCCCTGCAC TTGGCCCTTC TGTCTCTCAG CTCTCACCTT CCTGCTCCTG ACGACCAATA 2701 ACCCCACCAT CTACAAGCTC CCGCTCAGCA AAGTCACCTA CCCAGAGGCC AACCGCATCT 2761 ACTACCTGTC CCAGGAGAGA AACAGAAGGG CATCAATCAT AACAAAGTAT CAGGCCTACG 2821 ATGTCTCCTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 2881 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 2941 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATTTGGT TTATTTCAGT CCTTCAGCAC 3001 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt