Construct: ORF TRCN0000475541
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009592.1_s317c1
- Derived from:
- ccsbBroadEn_00553
- DNA Barcode:
- ACCCATCTACACCTGCGGGTAAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPC4 (2239)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475541
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2239 | GPC4 | glypican 4 | NM_001448.3 | 100% | 100% | |
| 2 | mouse | 14735 | Gpc4 | glypican 4 | NM_008150.2 | 90.7% | 94.4% | (many diffs) |
| 3 | mouse | 14735 | Gpc4 | glypican 4 | XM_011250978.2 | 82.2% | 85.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1734
- ORF length:
- 1668
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc acggttcggc ttgcccgcgc ttctctgcac cctggcagtg ctcagcgccg 121 cgctgctggc tgccgagctc aagtcgaaaa gttgctcgga agtgcgacgt ctttacgtgt 181 ccaaaggctt caacaagaac gatgcccccc tccacgagat caacggtgat catttgaaga 241 tctgtcccca gggttctacc tgctgctctc aagagatgga ggagaagtac agcctgcaaa 301 gtaaagatga tttcaaaagt gtggtcagcg aacagtgcaa tcatttgcaa gctgtctttg 361 cttcacgtta caagaagttt gatgaattct tcaaagaact acttgaaaat gcagagaaat 421 ccctgaatga tatgtttgtg aagacatatg gccatttata catgcaaaat tctgagctat 481 ttaaagatct cttcgtagag ttgaaacgtt actacgtggt gggaaatgtg aacctggaag 541 aaatgctaaa tgacttctgg gctcgcctcc tggagcggat gttccgcctg gtgaactccc 601 agtaccactt tacagatgag tatctggaat gtgtgagcaa gtatacggag cagctgaagc 661 ccttcggaga tgtccctcgc aaattgaagc tccaggttac tcgtgctttt gtagcagccc 721 gtactttcgc tcaaggctta gcggttgcgg gagatgtcgt gagcaaggtc tccgtggtaa 781 accccacagc ccagtgtacc catgccctgt tgaagatgat ctactgctcc cactgccggg 841 gtctcgtgac tgtgaagcca tgttacaact actgctcaaa catcatgaga ggctgtttgg 901 ccaaccaagg ggatctcgat tttgaatgga acaatttcat agatgctatg ctgatggtgg 961 cagagaggct agagggtcct ttcaacattg aatcggtcat ggatcccatc gatgtgaaga 1021 tttctgatgc tattatgaac atgcaggata atagtgttca agtgtctcag aaggttttcc 1081 agggatgtgg accccccaag cccctcccag ctggacgaat ttctcgttcc atctctgaaa 1141 gtgccttcag tgctcgcttc agaccacatc accccgagga acgcccaacc acagcagctg 1201 gcactagttt ggaccgactg gttactgatg tcaaggagaa actgaaacag gccaagaaat 1261 tctggtcctc ccttccgagc aacgtttgca acgatgagag gatggctgca ggaaacggca 1321 atgaggatga ctgttggaat gggaaaggca aaagcaggta cctgtttgca gtgacaggaa 1381 atggattagc caaccagggc aacaacccag aggtccaggt tgacaccagc aaaccagaca 1441 tactgatccT TCGTCAAATC ATGGCTCTTC GAGTGATGAC CAGCAAGATG AAGAATGCAT 1501 ACAATGGGAA CGACGTGGAC TTCTTTGATA TCAGTGATGA AAGTAGTGGA GAAGGAAGTG 1561 GAAGTGGCTG TGAGTATCAG CAGTGCCCTT CAGAGTTTGA CTACAATGCC ACTGACCATG 1621 CTGGGAAGAG TGCCAATGAG AAAGCCGACA GTGCTGGTGT CCGTCCTGGG GCACAGGCCT 1681 ACCTCCTCAC TGTCTTCTGC ATCTTGTTCC TGGTTATGCA GAGAGAGTGG AGATACCCAA 1741 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1801 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1861 TATCTTGTGG AAAGGACGAA CCCATCTACA CCTGCGGGTA AAGACGCGTT AAGTCgacaa 1921 tcaacctctg gattacaaaa tttgtgaaag att