Construct: ORF TRCN0000475551
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011740.1_s317c1
- Derived from:
- ccsbBroadEn_14490
- DNA Barcode:
- ACGAACACCAGTACCGGGAGCTGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TPTEP1 (387590)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475551
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 387590 | TPTEP1 | TPTE pseudogene 1 | NR_001591.1 | 18.3% | (many diffs) | |
| 2 | human | 7179 | TPTE | transmembrane phosphatase w... | NM_199260.3 | 16.5% | 13.8% | (many diffs) |
| 3 | human | 93492 | TPTE2 | transmembrane phosphoinosit... | NM_199254.2 | 15.7% | 13.2% | (many diffs) |
| 4 | human | 7179 | TPTE | transmembrane phosphatase w... | NM_199261.3 | 15.2% | 12.5% | (many diffs) |
| 5 | human | 7179 | TPTE | transmembrane phosphatase w... | NM_199259.3 | 15.1% | 13.1% | (many diffs) |
| 6 | human | 93492 | TPTE2 | transmembrane phosphoinosit... | NR_073485.1 | 11.1% | (many diffs) | |
| 7 | human | 220115 | TPTE2P3 | transmembrane phosphoinosit... | NR_002793.1 | 8.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 345
- ORF length:
- 276
- Sequence:
-
1 tcTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCACCAT GGATTCACTA CCAGATTCTA CTGTATGCTC TGACAACTAT GACCACAATG 121 GCAGCAAGAT TAAGAAAATT GTGCATTCAA TTATATCATC CTTTGCATTT GGACTATTTG 181 GAGTTTTCCT GGTCTTACTG GATGTCACTC TCGTCCTTGC CGACCTAATT TTCACTGACA 241 GCAAACTTTA TATTCCTTCG GAGTATCGTT CTATTTCTCT AGCTATTGCC TTATTTTTTC 301 TCATGGATGT TCTTCTTCGA GTATTTGTAG AAGGGAAACT GAACTTGCCA ACTTTCTTGT 361 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 421 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 481 GAAAGGACGA ACGAACACCA GTACCGGGAG CTGGACGCGT TAAGTCgaca atcaacctct 541 ggattacaaa atttgtgaaa gatt