Construct: ORF TRCN0000475571
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005166.1_s317c1
- Derived from:
- ccsbBroadEn_12778
- DNA Barcode:
- ACCAGAATCATGAAACTGATACCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- QRFPR (84109)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475571
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84109 | QRFPR | pyroglutamylated RFamide pe... | NM_198179.3 | 82.4% | 82.3% | 1_225del;654C>T;1031T>C |
| 2 | human | 84109 | QRFPR | pyroglutamylated RFamide pe... | XM_017008693.2 | 26.3% | 25.9% | (many diffs) |
| 3 | mouse | 229214 | Qrfpr | pyroglutamylated RFamide pe... | NM_198192.2 | 69.8% | 68.5% | (many diffs) |
| 4 | mouse | 243407 | C130060K24Rik | RIKEN cDNA C130060K24 gene | NM_175524.4 | 67.9% | 66% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1137
- ORF length:
- 1068
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcgcaccgtc accaacatct ttatctgctc cttggcgctc agtgacctgc 121 tcatcacctt cttctgcatt cccgtcacca tgctccagaa catttccgac aactggctgg 181 ggggtgcttt catttgcaag atggtgccat ttgtccagtc taccgctgtt gtgacagaaa 241 tcctcactat gacctgcatt gctgtggaaa ggcaccaggg acttgtgcat ccttttaaaa 301 tgaagtggca atacaccaac cgaagggctt tcacaatgct aggtgtggtc tggctggtgg 361 cagtcatcgt aggatcaccc atgtggcacg tgcaacaact tgagatcaaa tatgacttcc 421 tatatgaaaa ggaacacatc tgctgcttag aagagtggac cagccctgtg caccagaaga 481 tctacaccac cttcattctt gtcatcctct tcctcctgcc tcttatggtg atgcttattc 541 tgtacagtaa aattggttat gaactttgga taaagaaaag agttggggat ggttcagtgc 601 ttcgaactat tcatggaaaa gaaatgtcca aaatagccag gaagaagaaa cgagctgtca 661 ttatgatggt gacagtggtg gctctctttg ctgtgtgctg ggcaccattc catgttgtcc 721 atatgatgat tgaatacagt aattttgaaa aggaatatga tgatgtcaca atcaagatga 781 tttttgctat cgtgcaaatt attggatttt ccaactccat ctgtaatccc attGTCTATG 841 CATTTATGAA TGAAAACTTC AAAAAAAATG TTTCGTCTGC AGTTTGTTAT TGCATAGTAA 901 ATAAAACCTT CTCTCCAGCA CAAAGGCATG GAAATTCAGG AATTACAATG ATGCGGAAGA 961 AAGCAAAGTT TTCCCTCAGA GAGAATCCAG TGGAGGAAAC CAAAGGAGAA GCATTCAGTG 1021 ATGGCAACAT TGAAGTCAAA TTGTGTGAAC AGACAGAGGA GAAGAAAAAG CTCAAACGAC 1081 ATCTTGCTCT CTTTAGGTCT GAACTGGCTG AGAATTCTCC TTTAGACAGT GGGCATTTGC 1141 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1201 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1261 ATATATCTTG TGGAAAGGAC GAACCAGAAT CATGAAACTG ATACCCACGC GTTAAGTCga 1321 caatcaacct ctggattaca aaatttgtga aagatt