Construct: ORF TRCN0000475578
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000524.1_s317c1
- Derived from:
- ccsbBroadEn_09400
- DNA Barcode:
- TGAAAGCATAGCTTTGTCTAAATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ESCO1 (114799)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475578
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 114799 | ESCO1 | establishment of sister chr... | NM_052911.3 | 99.9% | 99.8% | 662C>T;1311G>A |
| 2 | human | 114799 | ESCO1 | establishment of sister chr... | XM_011525798.1 | 99.9% | 99.8% | 662C>T;1311G>A |
| 3 | human | 114799 | ESCO1 | establishment of sister chr... | XM_011525799.3 | 96.3% | 96.3% | 662C>T;1311G>A;1952_1953ins90 |
| 4 | human | 114799 | ESCO1 | establishment of sister chr... | XR_001753137.2 | 54.1% | (many diffs) | |
| 5 | human | 114799 | ESCO1 | establishment of sister chr... | XR_001753138.1 | 52.7% | (many diffs) | |
| 6 | mouse | 77805 | Esco1 | establishment of sister chr... | XM_006526353.3 | 35.3% | 34.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2589
- ORF length:
- 2520
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gatgtccatt caggagaaat caaaagagaa ttcctccaaa gttactaaaa 121 aaagtgacga taagaattca gaaacagaaa ttcaggattc tcaaaagaat ctagcaaaaa 181 aatcaggtcc aaaggagact ataaaatcac aggctaaatc ttccagtgaa agtaaaataa 241 atcagccaga attggaaaca cgcatgagta caaggtcatc aaaggcagca tctaatgata 301 aagctactaa atccattaat aaaaatacgg tgactgtgag gggatattca caagaatcta 361 caaaaaagaa attatctcag aaaaaattag tacatgaaaa ccctaaagca aatgaacagc 421 ttaaccggag atcacaaagg ctacaacaat taacagaggt ttcaagaagg tcgttacgca 481 gtagagaaat tcagggtcaa gttcaagcag ttaaacagag tttgccacca actaaaaaag 541 agcagtgtag cagtactcag agtaaatcta ataaaacaag tcaaaaacat gtgaagagaa 601 aagtactgga agtaaagtct gactctaaag aagatgaaaa tctagtaatt aatgaagtaa 661 taaattctcc caaagggaaa aaacgcaagg tagaacatca gacagcttgt gcttgtagtt 721 ctcaatgcat gcaaggatct gaaaagtgtc ctcagaagac tactagaaga gacgaaacga 781 aacctgtgcc tgtaacttct gaggtgaaaa gatcaaaaat ggctacttca gtggtcccga 841 aaaagaatga gatgaagaag tcggttcata cacaagtgaa tactaacaca acactcccaa 901 aaagtccaca gccatcagtg cctgaacaaa gtgataatga gctggagcaa gcaggaaaga 961 gcaaacgagg tagtattctc cagctctgtg aagaaattgc tggtgaaatt gagtcagata 1021 atgtagaggt aaaaaaggaa tcttcacaaa tggaaagtgt aaaggaagaa aagcccacag 1081 aaataaaatt ggaagagacc agtgttgaaa gacaaatact tcatcagaag gaaacaaatc 1141 aggatgtgca atgtaatcgt tttttcccaa gtagaaaaac aaagcctgtg aaatgtatac 1201 taaatggaat aaacagctca gccaagaaga actccaactg gactaaaatt aaactctcaa 1261 aatttaactc tgtgcagcac aataagttgg actctcaagt ttcccctaaa ttaggcttat 1321 tacgaaccag tttttcacca ccagctttag aaatgcatca tccagtgact caaagtacat 1381 ttttagggac aaagctacat gatagaaata taacttgcca gcaggaaaaa atgaaagaaa 1441 ttaattctga agaagtgaaa attaatgata ttacagtaga aattaataaa accacagaaa 1501 gggctcctga aaattgtcat ttggccaatg agataaaacc ttctgaccca ccattggata 1561 atcagatgaa acattctttt gattcagcat caaataagaa tttcagccaa tgtttggaat 1621 ccaagctaga aaacagtcca gtggaaaatg ttactgctgc ttcgactctg ctcagtcaag 1681 caaaaattga tacaggagag aataaatttc caggttcagc tccccaacag catagtattc 1741 tcagtaacca gacatctaaa agcagtgata acagggagac accacgaaat cattctttgc 1801 ctaagtgtaa ttcccatttg gagataacaa ttccaaagga cttgaaacta aaagaagcag 1861 agaaaactga tgaaaaacag ttgattatag atgcaggaca aaaaagattt ggagcagttt 1921 cttgtaatgt ttgtggaatg ctgtatacag cttcaaatcc agaagatgaa acacagcatc 1981 tgcttttcca caaccagttt ataagtgctg ttaaatatgt gggctggaag aaagaaagaa 2041 ttctggctga ataccctgat ggcaggataa taatggttct tcctgaagac ccaaagtatg 2101 ccctgaaaaa ggttgacgag attagagaga tggttgacaa tgatttaggt tttcaacagg 2161 ctccactaat gtgctattcc agaactaaaa cacttctctt catttccaat gacaaaaaag 2221 tagttggctg cctaattgcg gaacatatcc aatggggcta cagagttata gaagagaaac 2281 ttccagttat caggtcagaa gaagaaaaag tcagattTGA AAGGCAAAAA GCCTGGTGCT 2341 GCTCAACATT ACCAGAGCCT GCAATCTGCG GGATCAGTCG AATATGGGTA TTCAGCATGA 2401 TGCGTCGGAA GAAAATTGCT TCTCGCATGA TTGAATGCCT AAGGAGTAAC TTTATATATG 2461 GCTCATATTT GAGCAAAGAA GAAATTGCTT TCTCAGATCC CACTCCTGAT GGAAAGCTGT 2521 TTGCAACACA GTACTGTGGC ACTGGTCAAT TTCTGGTATA TAATTTTATT AATGGACAGA 2581 ATAGCACGTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 2641 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 2701 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATGAAAG CATAGCTTTG TCTAAATCAC 2761 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt