Construct: ORF TRCN0000475631

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002001.1_s317c1
Derived from:
ccsbBroadEn_02748
DNA Barcode:
ACCCCTCTGCTATAGTTACCAGGT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
BICD2 (23299)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475631
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23299 BICD2 BICD cargo adaptor 2 NM_001003800.2 100% 100%
2 human 23299 BICD2 BICD cargo adaptor 2 NM_015250.4 96.3% 96.2% 2468_2469insCGTAAGTCACA;2472_2473ins82
3 human 23299 BICD2 BICD cargo adaptor 2 XM_017014551.1 93.4% 93.3% 239_319del;2549_2550insCGTAAGTCACA;2553_2554ins82
4 mouse 76895 Bicd2 bicaudal D homolog 2 (Droso... NM_001039179.2 89.2% 94.7% (many diffs)
5 mouse 76895 Bicd2 bicaudal D homolog 2 (Droso... NM_029791.4 85.7% 91.2% (many diffs)
6 mouse 76895 Bicd2 bicaudal D homolog 2 (Droso... NM_001039180.2 77.4% 82.6% (many diffs)
7 mouse 76895 Bicd2 bicaudal D homolog 2 (Droso... XM_011244429.2 68.8% 70.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2631
ORF length:
2565
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtc ggcgccgtcg gaggaggagg agtacgcgcg gctggtgatg gaggcgcagc
121cggagtggct gcgcgccgag gtgaagcggc tgtcccacga gctggccgag accacgcgtg
181agaagatcca ggcggccgag tacgggctgg cggtgctcga ggagaagcac cagctcaagc
241tgcagttcga ggagctcgag gtggactatg aggctatccg cagcgagatg gagcagctca
301aggaggcctt tggacaagca cacacaaacc acaagaaggt ggctgctgac ggagagagcc
361gggaggagag cctgatccag gagtcggcct ccaaggagca gtactacgtg cggaaggtgc
421tagagctgca gacggagctg aagcagttgc gcaatgtcct caccaacacg cagtcggaga
481atgagcgcct ggcctctgtg gcccaggagc tgaaggagat caaccagaat gtggagatcc
541agcgtggccg cctgcgggat gacatcaagg agtacaaatt ccgggaagct cgtctgctgc
601aggactactc ggaactggag gaggagaaca tcagcctgca gaagcaagtg tctgtgctca
661gacagaacca ggtggagttt gagggcctca agcatgagat caagcgtctg gaggaggaga
721ccgagtacct caacagccag ctggaggatg ccatccgcct caaggagatc tcagagcggc
781agctggagga ggcgctggag accctgaaga cggagcgcga acagaagaac agcctgcgca
841aggagctgtc acactacatg agcatcaatg actccttcta caccagccac ctgcatgtct
901cgctggatgg cctcaagttc agtgacgatg ctgccgagcc caacaacgat gccgaggccc
961tggtcaatgg ctttgagcac ggcggcctgg ccaagctgcc actggacaac aagacctcca
1021cgcccaagaa ggagggcctc gcaccgccct cccccagcct cgtctccgac ctactcagtg
1081agctcaacat ctctgagatc cagaagctga agcagcagct gatgcagatg gagcgggaaa
1141aggcgggcct gctggcaacg ctgcaggaca cacagaagca gctggagcac acgcggggct
1201ccctgtcaga acagcaggag aaggtgaccc gcctcacaga gaatctgagt gccctgcggc
1261gcctgcaggc cagcaaggag cggcagacag ccctggacaa cgagaaggac cgtgacagcc
1321atgaggatgg ggactactac gaggtggaca tcaacgggcc tgagatcttg gcctgcaagt
1381accatgtggc tgtggctgag gctggcgagc tccgcgagca gctcaaggca ctgcgcagca
1441cgcacgaggc tcgtgaggcc cagcacgccg aggagaaggg ccgctatgag gctgagggcc
1501aggcactcac ggagaaggtc tccctgctag agaaggccag ccgccaggac cgcgagctgc
1561tggcccggct ggagaaggag ctaaagaagg tgagcgacgt cgccggcgag acacagggca
1621gcctgagtgt ggcccaggat gagctggtga ccttcagtga ggagctggcc aatctctacc
1681accacgtgtg catgtgcaac aatgagacac ccaaccgtgt catgctggac tactaccgcg
1741agggccaggg cggggccggc cgcaccagtc ccgggggccg caccagcccc gaggcgcgtg
1801gccggcgctc acccatcctc ctacccaagg ggctgctggc tcctgaggcg ggccgagcag
1861atggtgggac gggggacagc agcccctcgc ctggctcctc actgccatca cccctgagtg
1921acccacgccg ggagcccatg aacatctaca acctgatcgc tatcatccgt gaccagatca
1981agcacctgca ggcagccgtg gaccgcacca cggagctgtc acgccagcgc attgcctctc
2041aggagctggg ccccgccgtg gacaaggaca aggaagcgct tatggaggag atcctcaagc
2101tgaagtcgct gctcagcacc aagcgggagc agatcaccac gctgcgcact gtgctcaagg
2161ccaacaagca gacggccgag gtggcccttg ccaacctgaa gagcaagtat gagaatgaga
2221aggccatggt taccgagacc atgatgaagc tgcgcaatga gctcaaggcc ctcaaggagg
2281acgcagccac cTTCTCCTCG CTGCGTGCTA TGTTTGCCAC CAGGTGTGAC GAGTACATTA
2341CACAGCTGGA TGAGATGCAG CGGCAGCTGG CGGCTGCTGA GGACGAGAAG AAGACGCTGA
2401ACTCGCTGCT GCGCATGGCC ATCCAGCAGA AGCTGGCGCT GACCCAGCGG CTGGAGCTGC
2461TCGAGCTGGA CCATGAGCAG ACCCGGCGTG GCCGTGCCAA AGCCGCCCCG AAGACCAAGC
2521CAGCCACACC GAGCGTAAGT CACACCTGTG CCTGTGCCAG CGACAGGGCC GAGGGCACCG
2581GGCTGGCCAA CCAGGTGTTC TGCAGCGAGA AGCACAGCAT TTACTGTGAT TACCCAACTT
2641TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
2701CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
2761CTTGTGGAAA GGACGAACCC CTCTGCTATA GTTACCAGGT ACGCGTTAAG TCgacaatca
2821acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)