Construct: ORF TRCN0000475631
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002001.1_s317c1
- Derived from:
- ccsbBroadEn_02748
- DNA Barcode:
- ACCCCTCTGCTATAGTTACCAGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BICD2 (23299)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475631
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23299 | BICD2 | BICD cargo adaptor 2 | NM_001003800.2 | 100% | 100% | |
| 2 | human | 23299 | BICD2 | BICD cargo adaptor 2 | NM_015250.4 | 96.3% | 96.2% | 2468_2469insCGTAAGTCACA;2472_2473ins82 |
| 3 | human | 23299 | BICD2 | BICD cargo adaptor 2 | XM_017014551.1 | 93.4% | 93.3% | 239_319del;2549_2550insCGTAAGTCACA;2553_2554ins82 |
| 4 | mouse | 76895 | Bicd2 | bicaudal D homolog 2 (Droso... | NM_001039179.2 | 89.2% | 94.7% | (many diffs) |
| 5 | mouse | 76895 | Bicd2 | bicaudal D homolog 2 (Droso... | NM_029791.4 | 85.7% | 91.2% | (many diffs) |
| 6 | mouse | 76895 | Bicd2 | bicaudal D homolog 2 (Droso... | NM_001039180.2 | 77.4% | 82.6% | (many diffs) |
| 7 | mouse | 76895 | Bicd2 | bicaudal D homolog 2 (Droso... | XM_011244429.2 | 68.8% | 70.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2631
- ORF length:
- 2565
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc ggcgccgtcg gaggaggagg agtacgcgcg gctggtgatg gaggcgcagc 121 cggagtggct gcgcgccgag gtgaagcggc tgtcccacga gctggccgag accacgcgtg 181 agaagatcca ggcggccgag tacgggctgg cggtgctcga ggagaagcac cagctcaagc 241 tgcagttcga ggagctcgag gtggactatg aggctatccg cagcgagatg gagcagctca 301 aggaggcctt tggacaagca cacacaaacc acaagaaggt ggctgctgac ggagagagcc 361 gggaggagag cctgatccag gagtcggcct ccaaggagca gtactacgtg cggaaggtgc 421 tagagctgca gacggagctg aagcagttgc gcaatgtcct caccaacacg cagtcggaga 481 atgagcgcct ggcctctgtg gcccaggagc tgaaggagat caaccagaat gtggagatcc 541 agcgtggccg cctgcgggat gacatcaagg agtacaaatt ccgggaagct cgtctgctgc 601 aggactactc ggaactggag gaggagaaca tcagcctgca gaagcaagtg tctgtgctca 661 gacagaacca ggtggagttt gagggcctca agcatgagat caagcgtctg gaggaggaga 721 ccgagtacct caacagccag ctggaggatg ccatccgcct caaggagatc tcagagcggc 781 agctggagga ggcgctggag accctgaaga cggagcgcga acagaagaac agcctgcgca 841 aggagctgtc acactacatg agcatcaatg actccttcta caccagccac ctgcatgtct 901 cgctggatgg cctcaagttc agtgacgatg ctgccgagcc caacaacgat gccgaggccc 961 tggtcaatgg ctttgagcac ggcggcctgg ccaagctgcc actggacaac aagacctcca 1021 cgcccaagaa ggagggcctc gcaccgccct cccccagcct cgtctccgac ctactcagtg 1081 agctcaacat ctctgagatc cagaagctga agcagcagct gatgcagatg gagcgggaaa 1141 aggcgggcct gctggcaacg ctgcaggaca cacagaagca gctggagcac acgcggggct 1201 ccctgtcaga acagcaggag aaggtgaccc gcctcacaga gaatctgagt gccctgcggc 1261 gcctgcaggc cagcaaggag cggcagacag ccctggacaa cgagaaggac cgtgacagcc 1321 atgaggatgg ggactactac gaggtggaca tcaacgggcc tgagatcttg gcctgcaagt 1381 accatgtggc tgtggctgag gctggcgagc tccgcgagca gctcaaggca ctgcgcagca 1441 cgcacgaggc tcgtgaggcc cagcacgccg aggagaaggg ccgctatgag gctgagggcc 1501 aggcactcac ggagaaggtc tccctgctag agaaggccag ccgccaggac cgcgagctgc 1561 tggcccggct ggagaaggag ctaaagaagg tgagcgacgt cgccggcgag acacagggca 1621 gcctgagtgt ggcccaggat gagctggtga ccttcagtga ggagctggcc aatctctacc 1681 accacgtgtg catgtgcaac aatgagacac ccaaccgtgt catgctggac tactaccgcg 1741 agggccaggg cggggccggc cgcaccagtc ccgggggccg caccagcccc gaggcgcgtg 1801 gccggcgctc acccatcctc ctacccaagg ggctgctggc tcctgaggcg ggccgagcag 1861 atggtgggac gggggacagc agcccctcgc ctggctcctc actgccatca cccctgagtg 1921 acccacgccg ggagcccatg aacatctaca acctgatcgc tatcatccgt gaccagatca 1981 agcacctgca ggcagccgtg gaccgcacca cggagctgtc acgccagcgc attgcctctc 2041 aggagctggg ccccgccgtg gacaaggaca aggaagcgct tatggaggag atcctcaagc 2101 tgaagtcgct gctcagcacc aagcgggagc agatcaccac gctgcgcact gtgctcaagg 2161 ccaacaagca gacggccgag gtggcccttg ccaacctgaa gagcaagtat gagaatgaga 2221 aggccatggt taccgagacc atgatgaagc tgcgcaatga gctcaaggcc ctcaaggagg 2281 acgcagccac cTTCTCCTCG CTGCGTGCTA TGTTTGCCAC CAGGTGTGAC GAGTACATTA 2341 CACAGCTGGA TGAGATGCAG CGGCAGCTGG CGGCTGCTGA GGACGAGAAG AAGACGCTGA 2401 ACTCGCTGCT GCGCATGGCC ATCCAGCAGA AGCTGGCGCT GACCCAGCGG CTGGAGCTGC 2461 TCGAGCTGGA CCATGAGCAG ACCCGGCGTG GCCGTGCCAA AGCCGCCCCG AAGACCAAGC 2521 CAGCCACACC GAGCGTAAGT CACACCTGTG CCTGTGCCAG CGACAGGGCC GAGGGCACCG 2581 GGCTGGCCAA CCAGGTGTTC TGCAGCGAGA AGCACAGCAT TTACTGTGAT TACCCAACTT 2641 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2701 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2761 CTTGTGGAAA GGACGAACCC CTCTGCTATA GTTACCAGGT ACGCGTTAAG TCgacaatca 2821 acctctggat tacaaaattt gtgaaagatt