Construct: ORF TRCN0000475632
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017933.1_s317c1
- Derived from:
- ccsbBroadEn_06919
- DNA Barcode:
- GAGATCTTCCTTTCGAATGGCGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCL15 (6359)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475632
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6359 | CCL15 | C-C motif chemokine ligand 15 | NM_032965.6 | 100% | 100% | |
| 2 | human | 6368 | CCL23 | C-C motif chemokine ligand 23 | NM_145898.4 | 81.8% | 68.3% | (many diffs) |
| 3 | human | 6368 | CCL23 | C-C motif chemokine ligand 23 | NM_005064.6 | 72.7% | 59.8% | (many diffs) |
| 4 | human | 6368 | CCL23 | C-C motif chemokine ligand 23 | XR_429910.3 | 31.5% | (many diffs) | |
| 5 | human | 348249 | CCL15-CCL14 | CCL15-CCL14 readthrough (NM... | NR_027922.3 | 23.9% | 1_546del;886_1416del | |
| 6 | human | 348249 | CCL15-CCL14 | CCL15-CCL14 readthrough (NM... | NR_027921.3 | 23.1% | 1_546del;886_1464del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 408
- ORF length:
- 339
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaaggtctcc gtggctgccc tctcctgcct catgcttgtt gctgtccttg 121 gaTCCCAGGC CCAGTTCACA AATGATGCAG AGACAGAGTT AATGATGTCA AAGCTTCCAC 181 TGGAAAATCC AGTAGTTCTG AACAGCTTTC ACTTTGCTGC TGACTGCTGC ACCTCCTACA 241 TCTCACAAAG CATCCCGTGT TCACTCATGA AAAGTTATTT TGAAACGAGC AGCGAGTGCT 301 CCAAGCCAGG TGTCATATTC CTCACCAAGA AGGGGCGGCA AGTCTGTGCC AAACCCAGTG 361 GTCCGGGAGT TCAGGATTGC ATGAAAAAGC TGAAGCCCTA CTCAATATTG CCAACTTTCT 421 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 481 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 541 GTGGAAAGGA CGAGAGATCT TCCTTTCGAA TGGCGTTACG CGTTAAGTCg acaatcaacc 601 tctggattac aaaatttgtg aaagatt