Construct: ORF TRCN0000475639
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008203.1_s317c1
- Derived from:
- ccsbBroadEn_05281
- DNA Barcode:
- TTAGACGCAAGAACTCCGTTCGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATP6V0D2 (245972)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475639
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 245972 | ATP6V0D2 | ATPase H+ transporting V0 s... | NM_152565.1 | 100% | 100% | |
| 2 | mouse | 242341 | Atp6v0d2 | ATPase, H+ transporting, ly... | NM_175406.3 | 87.7% | 93.1% | (many diffs) |
| 3 | mouse | 242341 | Atp6v0d2 | ATPase, H+ transporting, ly... | XM_017320194.1 | 61.5% | 65.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1119
- ORF length:
- 1050
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctcgaaggt gcggagctgt acttcaacgt ggaccatggc tacctggagg 121 gcctggttcg aggatgcaag gccagcctcc tgacccagca agactatatc aacctggtcc 181 agtgtgagac cctagaagac ctgaaaattc atctccagac tactgattat ggtaactttt 241 tggctaatca cacaaatcct cttactgttt ccaaaattga cactgagatg aggaaaagac 301 tatgtggaga atttgagtat ttccggaatc attccctgga gcccctcagc acatttctca 361 cctatatgac gtgcagttat atgatagaca atgtgattct gctgatgaat ggtgcattgc 421 agaaaaaatc tgtgaaagaa attctgggga agtgccaccc cttgggccgt ttcacagaaa 481 tggaagctgt caacattgca gagacacctt cagatctctt taatgccatt ctgatcgaaa 541 cgccattagc tccattcttc caagactgca tgtctgaaaa tgctctagat gaactgaata 601 ttgaattgct acgcaataaa ctatacaagt cttaccttga ggcattctat aaattctgta 661 agaatcatgg tgatgtcaca gcagaagtta tgtgtcccat tcttgagttt gaggccgaca 721 gacgtgcttt tatcatcact cttaactcct ttggcactga attgagcaaa gaagaccgag 781 agaccctcta tccaaccttc ggcaaactct atccTGAGGG GTTGCGGCTG TTGGCTCAAG 841 CAGAAGACTT TGACCAGATG AAGAACGTAG CGGATCATTA CGGAGTATAC AAACCTTTAT 901 TTGAAGCTGT AGGTGGCAGT GGGGGAAAGA CATTGGAGGA CGTGTTTTAC GAGCGTGAGG 961 TACAAATGAA TGTGCTGGCA TTCAACAGAC AGTTCCACTA CGGTGTGTTT TATGCATATG 1021 TAAAGCTGAA GGAACAGGAA ATTAGAAATA TTGTGTGGAT AGCAGAATGT ATTTCACAGA 1081 GGCATCGAAC TAAAATCAAC AGTTACATTC CAATTTTATT GCCAACTTTC TTGTACAAAG 1141 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1201 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1261 ACGATTAGAC GCAAGAACTC CGTTCGCCAC GCGTTAAGTC gacaatcaac ctctggatta 1321 caaaatttgt gaaagatt