Construct: ORF TRCN0000475640
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007719.1_s317c1
- Derived from:
- ccsbBroadEn_10143
- DNA Barcode:
- AACCTGAAGTGCTCCCGGAGCTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CUEDC1 (404093)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475640
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 404093 | CUEDC1 | CUE domain containing 1 | NM_001271875.2 | 100% | 100% | |
| 2 | human | 404093 | CUEDC1 | CUE domain containing 1 | NM_001292025.1 | 100% | 100% | |
| 3 | human | 404093 | CUEDC1 | CUE domain containing 1 | XM_017024640.2 | 92.7% | 92.7% | 782_783ins84 |
| 4 | human | 404093 | CUEDC1 | CUE domain containing 1 | XM_017024641.2 | 71.4% | 57.6% | 0_1ins295;169_217del |
| 5 | human | 404093 | CUEDC1 | CUE domain containing 1 | XM_011524812.2 | 65.2% | 58.1% | 0_1ins370;94_142del |
| 6 | human | 404093 | CUEDC1 | CUE domain containing 1 | XR_934459.3 | 35.6% | 1_377del;1471_1489del;1555_3248del | |
| 7 | human | 404093 | CUEDC1 | CUE domain containing 1 | XR_001752509.2 | 34.6% | 1_377del;1470_1585del;1652_3345del | |
| 8 | human | 404093 | CUEDC1 | CUE domain containing 1 | XR_934460.3 | 34.5% | (many diffs) | |
| 9 | mouse | 103841 | Cuedc1 | CUE domain containing 1 | NM_198013.3 | 83.7% | 86.4% | (many diffs) |
| 10 | mouse | 103841 | Cuedc1 | CUE domain containing 1 | NM_001172099.1 | 83.2% | 85.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1224
- ORF length:
- 1158
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac cagcctgttc cgccggagca gcagcggcag cggcgggggt ggcaccgccg 121 gggcacgcgg gggcggggga ggcacggccg ccccccagga gctcaacaac agccggcctg 181 cccgccaggt gcgccgcctg gagttcaacc aggccatgga cgacttcaag accatgttcc 241 ccaacatgga ttacgacatc atcgaatgcg tgctgcgcgc caacagcggc gctgtggacg 301 ccaccatcga ccagctgctg cagatgaacc tggagggcgg tggcagcagc ggcggcgtct 361 atgaggacag ctccgactcg gaggacagca tccccccgga gatcttggaa aggactttgg 421 aacctgatag ctcggatgaa gagcccccac ctgtgtactc cccgccagcc taccacatgc 481 acgtgttcga ccggccctac cctctggctc ccccgactcc gcctccccgt atcgacgcgc 541 tgggctctgg agcccctaca agccagagac gctatcggaa ctggaaccca ccactgctgg 601 gcaaccttcc ggatgacttt ctccgcatcc tgccccagca gctggacagc atacagggta 661 acgctggggg ccccaagcct gggagtggag agggatgtcc acctgccatg gctgggccag 721 ggcccggaga ccaggagagc cgctggaagc agtacctgga ggacgagagg atcgcgcttt 781 tcctgcagaa cgaggagttc atgaaggagc tgcaacggaa ccgcgacttc ctcctcgctc 841 tggagagaga tcgattgaaa tacgaatccc agaaatctaa atccagcagc gtggctgtcg 901 gaaacgactt tggcttttcc tctcctgtcc caggaactgg cgacgccaac cccgctgtgt 961 ctgaagatgc cttattcagg gacaagctga aacacatggg aaagtccacc cggaggaaac 1021 tgtttgaact tgcccgagcc TTCTCAGAGA AGACCAAAAT GAGGAAGTCA AAGAGGAAAC 1081 ACTTGTTGAA GCATCAGTCG CTGGGGGCTG CCGCGTCAAC AGCCAACCTC CTGGATGATG 1141 TGGAGGGCCA CGCGTGTGAT GAAGACTTCC GGGGCAGGCG TCAGGAGGCA CCCAAGGTGG 1201 AGGAAGGCCT GCGAGAAGGA CAGTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1261 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1321 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA ACCTGAAGTG 1381 CTCCCGGAGC TAGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1441 att