Construct: ORF TRCN0000475649
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010638.1_s317c1
- Derived from:
- ccsbBroadEn_12910
- DNA Barcode:
- TAACTCCCCCACTGTCACAGCAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SYDE1 (85360)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475649
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 85360 | SYDE1 | synapse defective Rho GTPas... | NM_001300910.2 | 100% | 100% | |
| 2 | human | 85360 | SYDE1 | synapse defective Rho GTPas... | NM_033025.6 | 90.8% | 90.8% | 88_288del |
| 3 | human | 85360 | SYDE1 | synapse defective Rho GTPas... | XM_024451750.1 | 76.1% | 59.8% | 88_288del;1416_1417ins161;1881_1882ins163 |
| 4 | mouse | 71709 | Syde1 | synapse defective 1, Rho GT... | NM_027875.1 | 74.8% | 79.9% | (many diffs) |
| 5 | mouse | 71709 | Syde1 | synapse defective 1, Rho GT... | XM_006514148.1 | 53.1% | 55.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2070
- ORF length:
- 2004
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cgagccgcta ctcaggaaaa ccttctcccg cctgcggggc cgggagaaac 121 ttccccggaa aaagtcggac gccaaggagc gcgggcctgg ggtacctggc actggggagc 181 ccgccggcga gatctggtac aaccccatcc ctgaggaaga ccccagacct ccagcacctg 241 agcccccggg gccacagcct ggctcagctg agtcagaggg cctggccccc caaggtgcag 301 cccccgccag ccccccaacc aaagcctccc gcaccaagtc cccgggcccc gccaggcgcc 361 tctccataaa gatgaagaag ctgccggaac tgcggcgccg cctgagcctg cgaggccccc 421 gggctggcag ggagcgcgag agggctgccc ctgcgggctc cgtcatcagc cgctaccacc 481 tggacagcag cgtggggggc cccgggccgg cagcagggcc tgggggcacc cggagcccga 541 gggccggtta cctcagcgac ggggactcac cggagcgccc agctgggccc ccatcaccca 601 cctccttccg gccctacgag gtgggtcccg cagcccgggc acccccggcc gcactctggg 661 gccgcctcag cctgcacctg tacggtctcg gggggctgcg gccagcgccg ggggccaccc 721 ccagggacct ctgctgccta ctgcaagtgg atggggaggc cagggcccga acagggccac 781 tgcgaggggg gccggacttc ctgcggctgg accacacctt ccacctggag ctggaggccg 841 ccaggctcct gcgcgccctg gtgcttgcgt gggaccctgg cgtgagaagg caccggccct 901 gtgcccaggg caccgtgctg ctgcccacgg tcttccgagg gtgccaggcc caacagctgg 961 ccgtgcgcct ggagcctcag gggctgctgt atgccaagct gaccctgtcg gagcagcagg 1021 aagcccctgc cacagctgag ccccgcgtct ttgggctgcc cctgccactg ctggtggagc 1081 gggagcggcc ccccggccag gtgcccctca tcatccagaa gtgcgttggg cagatcgagc 1141 gccgagggct gcgggtagtg ggactgtacc gtctttgtgg ctcagcggca gtgaagaaag 1201 agcttcggga tgcctttgag cgggacagtg cagcggtctg cctatctgag gacctgtacc 1261 ccgatatcaa tgtcatcact ggcatcctca aggattatct tcgagagttg cccaccccac 1321 tcatcaccca gcccctgtat aaggtggtac tggaggccat ggcccgggac cccccaaaca 1381 gagttccccc caccactgag ggcacccgag ggctcctcag ctgcctgcca gatgtggaaa 1441 gggccacgct gacgcttctc ctggaccacc tgcgcctcgt ctcctccttc catgcctaca 1501 accgcatgac cccacagaac ttggccgtgt gcttcgggcc tgtgctgctg ccggcacgcc 1561 aggcgcccac aaggcctcgt gcccgcagct ccggcccagg ccttgccagt gcagtggact 1621 tcaagcacca catcgaggtg ctgcactacc tgctgcagtc ttggccagat ccccgcctgc 1681 cccgacaatc tccagatgtc gcgccttact tgcgacccaa acgacagcca cctctgcacc 1741 tgccgctggc agaccccgaa gtggtgactc ggccccgcgg tcgaggaggc cccgaaagcc 1801 ccccgagcaa ccgctacgcc ggcgactgga gcgtttgcgg gcgggacttc ctgccctgtg 1861 GGCGGGATTT CCTGTCCGGG CCAGACTACG ACCACGTGAC GGGCAGTGAC AGCGAGGACG 1921 AGGACGAGGA GGTCGGCGAG CCGAGGGTCA CCGGTGACTT CGAAGACGAC TTCGATGCGC 1981 CCTTCAACCC GCACCTGAAT CTCAAAGACT TCGACGCCCT CATCCTGGAT CTGGAGAGAG 2041 AGCTCTCCAA GCAAATCAAC GTGTGCCTCT GCCCAACTTT CTTGTACAAA GTGGTTGATA 2101 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 2161 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATAACT 2221 CCCCCACTGT CACAGCAAGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 2281 tgaaagatt