Construct: ORF TRCN0000475656
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017753.1_s317c1
- Derived from:
- ccsbBroadEn_09954
- DNA Barcode:
- GTCAGAGCATTTCGTGACATGCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CAVIN1 (284119)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475656
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 284119 | CAVIN1 | caveolae associated protein 1 | NM_012232.6 | 99.9% | 100% | 882G>A |
| 2 | human | 284119 | CAVIN1 | caveolae associated protein 1 | XM_005257242.4 | 50.7% | 41.7% | (many diffs) |
| 3 | mouse | 19285 | Cavin1 | caveolae associated 1 | NM_008986.2 | 90.4% | 92.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1236
- ORF length:
- 1170
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggaccccacg ctctatattg tcgagcggcc gcttcccggg taccccgacg 121 ccgaggcccc ggagccttcc tccgctgggg ctcaggcagc ggaggagccg tcgggggccg 181 gctcagaaga gctgatcaag tcggaccagg tgaacggcgt gctggtgctg agcctcctgg 241 acaaaatcat cggggccgta gaccagatcc agctgactca agcacagctg gaggagcggc 301 aggcggagat ggagggcgca gtgcagagca tccagggcga gctgagcaag ctgggcaagg 361 cgcacgccac cacgagcaat acggtgagca agctgctgga gaaggtgcgc aaggtcagcg 421 tcaacgtgaa gaccgtgcgc ggcagcctgg agcgccaggc ggggcagatc aagaagctgg 481 aggtcaacga ggccgagctg ctgcggcgcc gcaactttaa agtcatgatc taccaggatg 541 aagtgaagct gccggccaaa ctgagcatca gcaaatcgct gaaagagtcg gaggcgctgc 601 cagagaagga gggcgaggag ctgggcgagg gcgagcggcc cgaggaggac gcagcggcgc 661 tggagctttc gtcggacgag gcggtggagg ttgaggaggt tattgaggag tcccgcgcag 721 agcgtatcaa gcgcagcggc ctgcggcgcg tggacgactt caagaaggcc ttctccaagg 781 agaagatgga gaagaccaag gtgcgtaccc gcgagaacct ggagaagacg cgcctcaaga 841 ccaaggaaaa cctggagaag acgcggcaca ccctggagaa gcgcatgaac aagctgggca 901 cgcgcctggt gcccgccgag cggcgcgaga aactgaagac gtcgcgagac aagttgcgca 961 aatccttcac gcccgaccac gtggtgtacg cgcgctccaa gaccgcggtc tacaaggtgc 1021 cacccttcac cTTCCACGTC AAGAAGATCC GCGAGGGCCA GGTGGAAGTG CTCAAGGCCA 1081 CCGAGATGGT GGAGGTGGGC GCCGACGACG ACGAGGGCGG CGCGGAGCGC GGGGAGGCCG 1141 GCGACCTGCG GCGCGGGAGC AGCCCCGACG TGCACGCGCT GCTGGAGATC ACCGAGGAGT 1201 CGGACGCCGT GCTGGTGGAC AAGAGCGACA GCGACTGCCC AACTTTCTTG TACAAAGTGG 1261 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1321 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1381 AGTCAGAGCA TTTCGTGACA TGCTTACGCG TTAAGTCgac aatcaacctc tggattacaa 1441 aatttgtgaa agatt