Construct: ORF TRCN0000475664

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015331.1_s317c1
Derived from:
ccsbBroadEn_06652
DNA Barcode:
ATCTCGCGCCACCCTAAGAATCAA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NPAS1 (4861)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475664
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4861 NPAS1 neuronal PAS domain protein 1 NM_002517.4 99.9% 100% 306G>T
2 human 4861 NPAS1 neuronal PAS domain protein 1 XM_017026844.1 73.7% 48.7% (many diffs)
3 human 4861 NPAS1 neuronal PAS domain protein 1 XM_024451524.1 71.1% 53.4% (many diffs)
4 human 4861 NPAS1 neuronal PAS domain protein 1 XM_024451525.1 63.5% 63.5% 0_1ins528;275_277delAGC;544_723del
5 human 4861 NPAS1 neuronal PAS domain protein 1 XM_024451526.1 52% 46.9% 0_1ins528;159_160ins274;267_268ins47
6 human 4861 NPAS1 neuronal PAS domain protein 1 NM_001321086.2 39.6% 33.2% 0_1ins528;541_542ins47;702_703ins493
7 human 4861 NPAS1 neuronal PAS domain protein 1 XM_024451527.1 39.6% 33.2% 0_1ins528;541_542ins47;702_703ins493
8 mouse 18142 Npas1 neuronal PAS domain protein 1 NM_008718.2 83.6% 86.7% (many diffs)
9 mouse 18142 Npas1 neuronal PAS domain protein 1 XM_017322021.1 83.6% 86.7% (many diffs)
10 mouse 18142 Npas1 neuronal PAS domain protein 1 XM_006539614.3 83.5% 86.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1836
ORF length:
1770
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc ggccccctat cccggcagtg gcggcggaag cgaggtcaaa tgcgtgggag
121gccgcggcgc cagcgtcccc tgggactttc tacccgggct gatggtcaag gcgccgtccg
181gaccgtgcct gcaggcgcag cgcaaggaga agtcccggaa cgcggcgcgc tcgcggcgcg
241ggaaggagaa cctggagttc ttcgagctgg ccaagcttct cccgctgccc ggcgccatct
301ccagccagct ggacaaggct tccatcgtgc gcctcagcgt cacctacctc cgcctgcgcc
361ggttcgccgc tctgggggcg ccgccctggg ggctgagagc cgcggggccg ccagctggcc
421tcgccccagg ccgccgcggc cccgcagcgc tggtctccga agtcttcgag cagcacctgg
481gaggtcacat cttgcagtcc ctggatggct ttgtgttcgc cttgaaccag gaaggaaaat
541tcctctacat ctcagagaca gtctccatct atctgggtct ctcacaggtg gagatgacgg
601gcagcagcgt cttcgactac attcaccctg gggaccactc agaggtgctg gagcaactgg
661ggctgcggac gccgacgccc ggccccccaa ccccgccctc cgtctcctct tcctcctcct
721cttcctcttc gcttgcagat acccccgaga tcgaggccag cctcaccaag gtgcccccct
781cctccctggt ccaggagcgc tccttctttg tccgcatgaa atccacgctc accaagaggg
841ggctgcacgt caaggcctca gggtacaagg tcatccacgt gactgggcgc cttcgggccc
901acgccctggg ccttgtggcc ctcgggcaca cgttgccccc ggcccccctg gctgagctgc
961cactccatgg acacatgatc gtcttccgtc tcagcctggg tctcaccatc cttgcttgtg
1021agagcagagt cagcgaccac atggacctgg ggccctcaga gctggtgggc cgcagctgct
1081accagtttgt ccacggacag gacgccacga ggatccgcca gagccacgtg gacttgctgg
1141acaagggtca ggtgatgact ggttactacc gttggctgca gcgtgccggg ggcttcgtgt
1201ggctgcagtc tgtggccaca gtggctggga gcgggaagag ccccggggag caccatgtgc
1261tttgggtcag ccacgtgctc agccaagccg agggtggcca aactcctttg gatgccttcc
1321agcttccagc cagcgtggcc tgtgaggagg catccagccc ggggccagag cccacagagc
1381cggagcctcc gacggaaggg aagcaggctg ccccagcgga gaacgaggcc ccccagaccc
1441agggcaaacg catcaaagtg gagcccggcc cgagggaaac caaaggctcc gaggacagtg
1501gcgacgagga tccctccagc cacccggcca caccgaggcc cgagttcacc tctgtcatcc
1561gggcaggggt cctgaagcag gatccggtgc ggccatgggg cctggcgcct cccggggacc
1621ccccgcccac cctcctgcac gcgggcttcc tgccgccggt ggtgcggggc ctgtgcacac
1681ccggcaccat ccgcTACGGC CCCGCGGAGC TGGGCCTGGT GTACCCGCAC CTGCAGAGGC
1741TGGGTCCGGG CCCCGCGCTC CCGGAGGCCT TTTACCCGCC CCTGGGCCTG CCCTACCCGG
1801GGCCCGCGGG CACCAGGCTG CCGCGGAAGG GGGACTGCCC AACTTTCTTG TACAAAGTGG
1861TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
1921CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
1981AATCTCGCGC CACCCTAAGA ATCAAACGCG TTAAGTCgac aatcaacctc tggattacaa
2041aatttgtgaa agatt

Download FASTA (ORF) (Full)