Construct: ORF TRCN0000475664
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015331.1_s317c1
- Derived from:
- ccsbBroadEn_06652
- DNA Barcode:
- ATCTCGCGCCACCCTAAGAATCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NPAS1 (4861)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475664
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4861 | NPAS1 | neuronal PAS domain protein 1 | NM_002517.4 | 99.9% | 100% | 306G>T |
| 2 | human | 4861 | NPAS1 | neuronal PAS domain protein 1 | XM_017026844.1 | 73.7% | 48.7% | (many diffs) |
| 3 | human | 4861 | NPAS1 | neuronal PAS domain protein 1 | XM_024451524.1 | 71.1% | 53.4% | (many diffs) |
| 4 | human | 4861 | NPAS1 | neuronal PAS domain protein 1 | XM_024451525.1 | 63.5% | 63.5% | 0_1ins528;275_277delAGC;544_723del |
| 5 | human | 4861 | NPAS1 | neuronal PAS domain protein 1 | XM_024451526.1 | 52% | 46.9% | 0_1ins528;159_160ins274;267_268ins47 |
| 6 | human | 4861 | NPAS1 | neuronal PAS domain protein 1 | NM_001321086.2 | 39.6% | 33.2% | 0_1ins528;541_542ins47;702_703ins493 |
| 7 | human | 4861 | NPAS1 | neuronal PAS domain protein 1 | XM_024451527.1 | 39.6% | 33.2% | 0_1ins528;541_542ins47;702_703ins493 |
| 8 | mouse | 18142 | Npas1 | neuronal PAS domain protein 1 | NM_008718.2 | 83.6% | 86.7% | (many diffs) |
| 9 | mouse | 18142 | Npas1 | neuronal PAS domain protein 1 | XM_017322021.1 | 83.6% | 86.7% | (many diffs) |
| 10 | mouse | 18142 | Npas1 | neuronal PAS domain protein 1 | XM_006539614.3 | 83.5% | 86.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1836
- ORF length:
- 1770
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggccccctat cccggcagtg gcggcggaag cgaggtcaaa tgcgtgggag 121 gccgcggcgc cagcgtcccc tgggactttc tacccgggct gatggtcaag gcgccgtccg 181 gaccgtgcct gcaggcgcag cgcaaggaga agtcccggaa cgcggcgcgc tcgcggcgcg 241 ggaaggagaa cctggagttc ttcgagctgg ccaagcttct cccgctgccc ggcgccatct 301 ccagccagct ggacaaggct tccatcgtgc gcctcagcgt cacctacctc cgcctgcgcc 361 ggttcgccgc tctgggggcg ccgccctggg ggctgagagc cgcggggccg ccagctggcc 421 tcgccccagg ccgccgcggc cccgcagcgc tggtctccga agtcttcgag cagcacctgg 481 gaggtcacat cttgcagtcc ctggatggct ttgtgttcgc cttgaaccag gaaggaaaat 541 tcctctacat ctcagagaca gtctccatct atctgggtct ctcacaggtg gagatgacgg 601 gcagcagcgt cttcgactac attcaccctg gggaccactc agaggtgctg gagcaactgg 661 ggctgcggac gccgacgccc ggccccccaa ccccgccctc cgtctcctct tcctcctcct 721 cttcctcttc gcttgcagat acccccgaga tcgaggccag cctcaccaag gtgcccccct 781 cctccctggt ccaggagcgc tccttctttg tccgcatgaa atccacgctc accaagaggg 841 ggctgcacgt caaggcctca gggtacaagg tcatccacgt gactgggcgc cttcgggccc 901 acgccctggg ccttgtggcc ctcgggcaca cgttgccccc ggcccccctg gctgagctgc 961 cactccatgg acacatgatc gtcttccgtc tcagcctggg tctcaccatc cttgcttgtg 1021 agagcagagt cagcgaccac atggacctgg ggccctcaga gctggtgggc cgcagctgct 1081 accagtttgt ccacggacag gacgccacga ggatccgcca gagccacgtg gacttgctgg 1141 acaagggtca ggtgatgact ggttactacc gttggctgca gcgtgccggg ggcttcgtgt 1201 ggctgcagtc tgtggccaca gtggctggga gcgggaagag ccccggggag caccatgtgc 1261 tttgggtcag ccacgtgctc agccaagccg agggtggcca aactcctttg gatgccttcc 1321 agcttccagc cagcgtggcc tgtgaggagg catccagccc ggggccagag cccacagagc 1381 cggagcctcc gacggaaggg aagcaggctg ccccagcgga gaacgaggcc ccccagaccc 1441 agggcaaacg catcaaagtg gagcccggcc cgagggaaac caaaggctcc gaggacagtg 1501 gcgacgagga tccctccagc cacccggcca caccgaggcc cgagttcacc tctgtcatcc 1561 gggcaggggt cctgaagcag gatccggtgc ggccatgggg cctggcgcct cccggggacc 1621 ccccgcccac cctcctgcac gcgggcttcc tgccgccggt ggtgcggggc ctgtgcacac 1681 ccggcaccat ccgcTACGGC CCCGCGGAGC TGGGCCTGGT GTACCCGCAC CTGCAGAGGC 1741 TGGGTCCGGG CCCCGCGCTC CCGGAGGCCT TTTACCCGCC CCTGGGCCTG CCCTACCCGG 1801 GGCCCGCGGG CACCAGGCTG CCGCGGAAGG GGGACTGCCC AACTTTCTTG TACAAAGTGG 1861 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1921 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1981 AATCTCGCGC CACCCTAAGA ATCAAACGCG TTAAGTCgac aatcaacctc tggattacaa 2041 aatttgtgaa agatt