Construct: ORF TRCN0000475708
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002868.1_s317c1
- Derived from:
- ccsbBroadEn_06467
- DNA Barcode:
- TAGCTGTTACGCTGACAGCTCGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ANOS1 (3730)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475708
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3730 | ANOS1 | anosmin 1 | NM_000216.4 | 99.9% | 99.8% | 1600G>A;1833C>T |
| 2 | human | 3730 | ANOS1 | anosmin 1 | XM_005274501.4 | 60.8% | 58.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2109
- ORF length:
- 2040
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtgcccggg gtgcccggcg cggtcctgac cctctgcctc tggctggcgg 121 cctccagcgg ctgcctggcg gccggccccg gcgcggctgc tgcgcggcgg ctggacgagt 181 cgctgtctgc cgggagcgtc cagcgcgctc gctgcgcctc caggtgcctg agcctgcaga 241 tcactcgcat ctccgccttc ttccagcact tccagaacaa tggttccctg gtttggtgcc 301 agaatcacaa gcaatgttct aagtgcctgg agccctgcaa ggaatcaggg gacctgagga 361 aacaccagtg ccaaagcttt tgtgagcctc tcttccccaa gaagagctac gaatgcttga 421 ccagctgtga gttcctcaaa tacatcctgt tggtgaagca gggggactgt ccggctcctg 481 agaaagccag tggatttgcg gccgcctgtg ttgaaagctg cgaagttgac aatgagtgct 541 ctggggtgaa gaaatgttgt tcgaatgggt gtggacacac ctgtcaagta cccaagactc 601 tgtacaaagg tgtccccctg aagcccagaa aagagttacg atttacagaa ctgcagtctg 661 gacagctgga ggttaagtgg tcctcgaaat tcaatatttc tattgagcct gtgatctatg 721 tggtacaaag aagatggaat tatggaatcc atcctagcga agatgacgcc actcactggc 781 agacagtggc ccagaccaca gacgagcgag ttcaactgac tgacataaga cccagccgat 841 ggtaccagtt tcgagtggct gctgtgaatg tgcatggaac tcgaggcttc actgccccca 901 gcaaacactt ccgttcttcc aaagatccat ctgccccacc agcaccggct aacctccggc 961 tggccaactc caccgtcaac agtgatggga gtgtgaccgt cactatagtt tgggatctcc 1021 ccgaggagcc ggacatccct gtgcatcatt acaaggtctt ttggagctgg atggtcagca 1081 gtaagtctct tgtcccaaca aagaagaagc ggagaaagac tacggatggg tttcaaaatt 1141 ctgtgatcct ggagaaactc cagccagact gtgactatgt tgtggaattg caagccataa 1201 cgtactgggg acagacacgg ctgaagagtg caaaggtgtc ccttcacttc acatcgacac 1261 atgcaaccaa caacaaagaa cagcttgtga aaactagaaa aggtggaatt caaacacaac 1321 tcccttttca aagacgacga cccactcgcc cgctggaagt cggagctccc ttctatcagg 1381 atggccaact gcaagttaaa gtctactgga agaagacaga agatcccact gtcaaccgat 1441 atcatgtgcg gtggtttcct gaagcgtgtg cccacaacag aacaaccgga tcagaggcat 1501 catctggcat gacccacgaa aattacataa ttcttcaaga tctgtcattt tcctgcaagt 1561 ataaggtgac tgtccaacca atacggccaa aaagtcactc caaggcagaa gctgttttct 1621 tcactactcc accatgctct gctcttaagg ggaagagcca caagcctatt ggctgcctgg 1681 gcgaagcagg tcatgttctt tctaaggtgc tagctaagcc tgagaacctt tctgcttcat 1741 tcatcgtcca ggatgtgaac atcaccggtc acttttcttg gaagatggcc aaggccaatc 1801 tctatcagcc catgactggg tttcaagtga cttgggctga ggtcactacg gaaagcagac 1861 agaacagcct acccaacagc attatttcac agtcccagat tctgccttcc gatCATTATG 1921 TCCTAACAGT GCCCAATCTG AGACCATCTA CTCTTTACCG ACTGGAAGTG CAAGTGCTGA 1981 CCCCAGGAGG GGAGGGGCCG GCCACCATCA AGACGTTCCG GACGCCGGAG CTCCCACCCT 2041 CTTCAGCACA CAGATCTCAT CTTAAGCATC GTCATCCACA TCATTACAAG CCTTCTCCAG 2101 AAAGATACTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 2161 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 2221 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATAGCTG TTACGCTGAC AGCTCGATAC 2281 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt