Construct: ORF TRCN0000475721
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015861.1_s317c1
- Derived from:
- ccsbBroadEn_07665
- DNA Barcode:
- CCTGATGAACCGTGAGTCAGTAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TSBP1 (10665)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475721
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10665 | TSBP1 | testis expressed basic prot... | NM_006781.5 | 99.6% | 98.9% | (many diffs) |
| 2 | human | 10665 | TSBP1 | testis expressed basic prot... | XM_024446307.1 | 92.4% | 91.7% | (many diffs) |
| 3 | human | 10665 | TSBP1 | testis expressed basic prot... | NM_001286474.2 | 92.1% | 91.8% | (many diffs) |
| 4 | human | 10665 | TSBP1 | testis expressed basic prot... | NM_001286475.2 | 92% | 91.9% | (many diffs) |
| 5 | human | 10665 | TSBP1 | testis expressed basic prot... | XM_017010182.1 | 70.7% | 70.3% | (many diffs) |
| 6 | human | 10665 | TSBP1 | testis expressed basic prot... | XM_017010183.1 | 19.3% | 4.2% | (many diffs) |
| 7 | human | 10665 | TSBP1 | testis expressed basic prot... | XM_024446306.1 | 17.8% | 3.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1755
- ORF length:
- 1689
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac agtcttggaa ataactttgg ctgtcatcct gactctactg ggacttgcca 121 tcctggctat tttgttaaca agatgggcac gacgtaagca aagtgaaatg catatctcca 181 gatacagttc agaacaaagt gctagacttc tggactatga ggatggtaga ggatcccgac 241 atgcatattc aacacaaagt gacacttcat gtgataaccg agagagatcc aaaagagatt 301 acacaccatc aaccaactct ctagtgtcta tggcatctaa gttctccctg ggacaaacag 361 aactcattct tcttttgatg tgttttattt tagcactgtc tcgatcaagt attggtagta 421 taaaatgttt acaaacaact gaagaacttc cttccagaac tgcaggagcc atgatgcaat 481 tcacagcccc tattcccgga gctacaggac ctatcaagct ctctcaaaaa accattgtgc 541 aaactccagg acctattgta caatatcctg gacccaatgc tggtccacct tcagcacccc 601 gcggtccacc catggcaccc ataataattt cacagagaac cgcaagaata cctcaagttc 661 acactatgga cagttctgga aaaatcacac tgactcctgt ggttatatta acaggttaca 721 tggatgaaga acttgcaaaa aaaccttgtt ccaaaatcca gattctaaaa tgtggaggca 781 ctgcaaggtc tcagaatagc cgagaagaaa acaaggaagc actaaagaat gacatcatat 841 ttacgaattc tgtagaatcc ttgaaatcag cacacataaa ggagccagaa agagaaggaa 901 aaggcactga tttagagaaa gacaaaatag gaatggaggt caaggtagac agtgacgctg 961 gaataccaaa aagacaggaa acccaactaa aaatcagtga gatgagtata ccacaaggac 1021 agggagccca aataaagaaa agtgtgtcag atgtaccaag aggacaggag tcccaagtaa 1081 agaagagtga gtcaggtgtc ccaaaaggac aagaagccca agtaacgaag agtgggttgg 1141 ttgtactgaa aggacaggaa gcccaggtag agaagagtga gatgggtgtg ccaagaagac 1201 aggaatccca agtaaagaag agtcagtctg gtgtctcaaa gggacaggaa gcccaggtaa 1261 agaagaggga gtcagttgta ctgaaaggac aggaagccca ggtagagaag agtgagttga 1321 aggtaccaaa aggacaagaa ggccaagtag agaagactga ggcagatgtg ccaaaggaac 1381 aagaggtcca agaaaagaag agtgaggcag gtgtactgaa aggaccagaa TCCCAAGTAA 1441 AGAACACTGA GGTGAGTGTA CCAGAAACAC TGGAATCCCA AGTAAAGAAG AGTGAGTCAG 1501 GTGTACTAAA AGGACAGGAA GCCCAAGAAA AGAAGGAGAG TTTTGAGGAT AAAGGAAATA 1561 ATGATAAAGA AAAGGAGAGA GATGCAGAGA AAGATCCAAA TAAAAAAGAA AAAGGTGACA 1621 AAAACACAAA AGGTGACAAA GGAAAGGACA AAGTTAAAGG AAAGAGAGAA TCAGAAATCA 1681 ATGGTGAAAA ATCAAAAGGC TCGAAAAGGG CGAAGGCAAA TACAGGAAGG AAGTACAACA 1741 AAAAAGTGGA AGAGTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1801 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1861 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CCTGATGAAC CGTGAGTCAG 1921 TAACACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt