Construct: ORF TRCN0000475738
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010970.1_s317c1
- Derived from:
- ccsbBroadEn_00309
- DNA Barcode:
- TTTCACCCTCTCATCTATCGTTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHRNB2 (1141)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475738
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1141 | CHRNB2 | cholinergic receptor nicoti... | NM_000748.3 | 100% | 100% | |
| 2 | human | 1141 | CHRNB2 | cholinergic receptor nicoti... | XM_017000180.2 | 66.1% | 66.1% | 0_1ins510 |
| 3 | human | 1141 | CHRNB2 | cholinergic receptor nicoti... | XR_001736952.2 | 15.4% | 1_252del;1759_9723del | |
| 4 | mouse | 11444 | Chrnb2 | cholinergic receptor, nicot... | NM_009602.4 | 87.5% | 94% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1575
- ORF length:
- 1506
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcccggcgc tgcggccccg tggcgctgct ccttggcttc ggcctcctcc 121 ggctgtgctc aggggtgtgg ggtacggata cagaggagcg gctggtggag catctcctgg 181 atccttcccg ctacaacaag cttatccgcc cagccaccaa tggctctgag ctggtgacag 241 tacagcttat ggtgtcactg gcccagctca tcagtgtgca tgagcgggag cagatcatga 301 ccaccaatgt ctggctgacc caggagtggg aagattatcg cctcacctgg aagcctgaag 361 agtttgacaa catgaagaaa gttcggctcc cttccaaaca catctggctc ccagatgtgg 421 tcctgtacaa caatgctgac ggcatgtacg aggtgtcctt ctattccaat gccgtggtct 481 cctatgatgg cagcatcttc tggctgccgc ctgccatcta caagagcgca tgcaagattg 541 aagtaaagca cttcccattt gaccagcaga actgcaccat gaagttccgt tcgtggacct 601 acgaccgcac agagatcgac ttggtgctga agagtgaggt ggccagcctg gacgacttca 661 cacctagtgg tgagtgggac atcgtggcgc tgccgggccg gcgcaacgag aaccccgacg 721 actctacgta cgtggacatc acgtatgact tcatcattcg ccgcaagccg ctcttctaca 781 ccatcaacct catcatcccc tgtgtgctca tcacctcgct agccatcctt gtcttctacc 841 tgccatccga ctgtggcgag aagatgacgt tgtgcatctc agtgctgctg gcgctcacgg 901 tcttcctgct gctcatctcc aagatcgtgc ctcccacctc cctcgacgtg ccgctcgtcg 961 gcaagtacct catgttcacc atggtgcttg tcaccttctc catcgtcacc agcgtgtgcg 1021 tgctcaacgt gcaccaccgc tcgcccacca cgcacaccat ggcgccctgg gtgaaggtcg 1081 tcttcctgga gaagctgccc gcgctgctct tcatgcagca gccacgccat cattgcgccc 1141 gtcagcgcct gcgcctgcgg cgacgccagc gtgagcgcga gggcgctgga gccctcttct 1201 tccgcgaagc cccaggggcc gactcctgca cgtgcttcgt caaccgcgcg tcggtgcagg 1261 ggttggccgg ggccttcggg gctgagcctg caccagtggc gggccccggg cgctcagggg 1321 agccgtgtgg ctgtggcctc cgggaggcgg tggacggcgt gcgcttcatc gcagaccaca 1381 tgcggagcga ggacgatgac cagagcgtga gtgaggactg gaagtacgtc gccatggtga 1441 tcgaccgcct cttcctctgg atctttgtct ttgtctgtgt ctttggcacc atcggcatgt 1501 tcctgcagcc tctcttccag aactacacca ccaccacctt cctccactca gaccacTCAG 1561 CCCCCAGCTC CAAGTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1621 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1681 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TTTCACCCTC TCATCTATCG 1741 TTTAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt