Construct: ORF TRCN0000475752
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015019.1_s317c1
- Derived from:
- ccsbBroadEn_09944
- DNA Barcode:
- ACCTACACTGGTCACGGAGATTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GXYLT1 (283464)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475752
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 283464 | GXYLT1 | glucoside xylosyltransferase 1 | NM_173601.2 | 99.9% | 99.7% | 631G>A |
| 2 | human | 283464 | GXYLT1 | glucoside xylosyltransferase 1 | NM_001099650.2 | 92.8% | 92.7% | 219_220ins93;538G>A |
| 3 | human | 283464 | GXYLT1 | glucoside xylosyltransferase 1 | XM_017019211.1 | 73.7% | 73.6% | 0_1ins345;286G>A |
| 4 | human | 100132541 | GXYLT1P3 | glucoside xylosyltransferas... | NR_121572.1 | 44.6% | (many diffs) | |
| 5 | mouse | 223827 | Gxylt1 | glucoside xylosyltransferase 1 | NM_001346785.1 | 84.2% | 86.3% | (many diffs) |
| 6 | mouse | 223827 | Gxylt1 | glucoside xylosyltransferase 1 | XM_006520848.1 | 81.3% | 83.1% | (many diffs) |
| 7 | mouse | 223827 | Gxylt1 | glucoside xylosyltransferase 1 | XM_006520849.1 | 77.5% | 79.5% | (many diffs) |
| 8 | mouse | 223827 | Gxylt1 | glucoside xylosyltransferase 1 | NM_001033275.4 | 70.2% | 68.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1389
- ORF length:
- 1320
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcggcgctac ctgcgcgtcg tggtgctgtg tgtggcctgc ggcttctgct 121 cgctccttta cgctttcagc cagctcgccg tgtccctgga agaaggaacg ggcggcggtg 181 gcgggaagcc gcaggccgcg gtggcttcct ggctcgcggg cggcggacgc ggcgccgtga 241 gaggcgccgg cgtcgcgggc cccgcagcgc atcccggcgt gtcggacagg tgtaaagatt 301 tctctctgtg ttactggaat ccctattgga tgctgccctc tgatgtttgt ggaatgaact 361 gcttttggga agcggctttt aggtacagtc tgaaaataca gcctgttgag aaaatgcatc 421 tagctgtagt tgcctgtggt gaaagactgg aagaaactat gaccatgttg aagtcagcta 481 tcattttcag catcaaacct cttcaattcc atatttttgc tgaagatcag ctacatcata 541 gctttaaagg cagacttgac aactggtcat ttctacaaac atttaattat acgttatacc 601 ccataacctt tccaagtgag aatgcagcag agtggaaaaa actctttaaa ccatgtgctt 661 cgcagagatt gttcttgccg ttaatcctga aagaagttaa ctcactattg tatgtcgaca 721 ctgatatcct ttttttacga ccagttgatg atatttggtc tttactaaag aaatttaatt 781 ccacacaaat tgctgcaatg gcaccagaac atgaggaacc tcgaatagga tggtataatc 841 gctttgctag gcatccatat tatggaaaaa ctggagtaaa ctctggagtt atgttgatga 901 acatgactcg aatgagaagg aagtatttca agaatgatat gacaactgta cgactacaat 961 ggggagatat acttatgcca ttgcttaaaa aatacaaact aaacatcaca tggggtgatc 1021 aagatctatt gaatatcgtg ttttttcata atccagaaag cctttttgtt tttccgtgtc 1081 aatggaatta tcgaccagat cattGTATAT ATGGAAGCAA TTGCCAAGAA GCAGAAGAAG 1141 GAGGAATCTT TATTCTTCAT GGGAACAGAG GTGTTTACCA TGACGATAAG CAACCAGCAT 1201 TTAGAGCTGT TTATGAAGCA CTGAGAAATT GTTCTTTTGA AGATGACAAC ATCCGTTCCT 1261 TATTAAAACC TTTAGAACTG GAACTACAAA AAACAGTGCA TACATACTGT GGAAAAATTT 1321 ACAAAATATT TATCAAACAA CTAGCAAAAA GTGTAAGAGA TCGTTATGCC AGATCACCAA 1381 AGGAAAAGTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1441 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1501 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAACCTAC ACTGGTCACG GAGATTGTAC 1561 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt