Construct: ORF TRCN0000475761
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002136.1_s317c1
- Derived from:
- ccsbBroadEn_08621
- DNA Barcode:
- GGCAGGCGACAGGCAGGCTTGTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCDHGA2 (56113)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475761
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56113 | PCDHGA2 | protocadherin gamma subfami... | NM_032009.3 | 99.9% | 100% | 552G>A |
| 2 | human | 56113 | PCDHGA2 | protocadherin gamma subfami... | NM_018915.4 | 87.2% | 86.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2538
- ORF length:
- 2469
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggctctg caaaagttgc cacactgcag aaagctggtc ctgctgtgct 121 tccttttggc gaccctgtgg gaggccaggg ccgggcagat tcgctattct gtgcgggaag 181 agatcgacag aggctccttc gtaggcaaca tcgccaagga cttgggtttg gagcccctgg 241 cactggcaga gcagggagtc cgcatcgtct ccagaggtag gtcccagctc tttgctctga 301 acccgcgaag cggcagcttg gtcactgcga acaggataga ccgggaggag ctctgcgctc 361 agagcgcacc ctgtctgttg aattttaaca ttctgctgga ggataaattg actatttatt 421 cagtagaggt ggaaataaca gatattaacg ataatgcccc tcgctttgga gtagaggaac 481 tggagctaaa aatcagtgaa accactacgc caggattccg gattcctctt aagaatgcgc 541 atgatgcaga cgtaggtgag aacgcccttc agaagtacgc actcaaccca aatgaccact 601 tctccctgga cgtgcgaaga ggagctgatg ggaacaagta cccagaactg gtgctggagc 661 gctctctgga ccgcgaggaa gaggctgttc accacctcgt tctcgtggct tctgatgggg 721 gtgacccagt gctatctggc acctcccgca tctgcgtgaa ggtcctggat gcgaacgaca 781 atgcgcctgt ttttacacag cccgagtacc gcataagcat tccggagaat acgctcgtgg 841 gcacccggat actcacggtg accgccactg acgcagatga gggctactac gctcaagtgg 901 tatattttct agagaaaagc cctggagaaa cctcagaggt atttgagctt aagtcaacat 961 ctggagaact gacaatcata aaagatctag attatgagga tgctacattc catgaaattg 1021 atattgaagc tcaggatggt ccgggccttc taaccagagc gaaggttatc gtcacggttc 1081 tggatgtgaa tgacaatgcc ccagaatttt acatgacatc tgctactagc tcagtttctg 1141 aagactctct tccaggaacc ataattgggc tttttaatgt acatgataga gactctgggc 1201 agaacgcatt caccacctgt tcactccccg aggatcttcc ttttaagtta gaaaagtcag 1261 tagacaatta ctaccgactg gttacaacca gagcccttga cagggaacag ttttcctttt 1321 acaacatcac tctaaccgct aaagatggag ggaacccctc cctgtccacg gatgctcaca 1381 ttttgctcca ggtggcagac atcaacgaca acgcacccgc cttctcccgc acatcctact 1441 ccacctacat tcccgaaaac aaccccagag gagcctctgt cttctcagtg acggcccatg 1501 accccgacag caacgacaat gctcatgtaa cttactcttt cgcggaggac actgttcagg 1561 gggcaccctt atcctcttac atctctatca actccgacac tggagtactc tatgcactgc 1621 gctcctttga ttatgagcag ttgcgagact tgcaagtgtg ggtgatagcg cgggacagcg 1681 ggaaccctcc actcagtagc aatgtatcat taagcctgtt cgtgctggac cagaacgaca 1741 acgcgcccga gatcctgtac cctgccttcc ccacagacgg ttccactggc gtggagctgg 1801 cgccccgctc cgcagagccc ggctacctgg tgaccaaggt ggtggcggtg gacagagact 1861 cgggccagaa cgcctggctg tcttaccacc tgctcaaggc cagcgagccg ggactcttct 1921 cggtgggtct gcacacgggc gaggtgcgca cggcgcgagc cctgctggac agagacgcgc 1981 tcaagcagag cctcgtggtg gccatccagg accacggcca gccccctctc tccgccactg 2041 tcacgctcac cgtggccgtg gccgacagga tccccgacat cctggccgac ctgggcagcc 2101 tcgagccctc cgccataccc aacgattcgg acctcactct gtacctggtg gtggcggtgg 2161 ccgcggtctc ctgcgtcttc ctggccttcg tcatcgtgtt gctggcgcac aggctgcggc 2221 gctggcacaa gtcacgccTG CTGCAGGCTT CAGGAGGCAG CTTGACAGGC ATGCAGAGCT 2281 CGCACTTTGT GGGCGTGGAC GGGGTTCGGG CTTTCCTGCA GACCTATTCC CACGAGGTCT 2341 CCCTCACTGC GGACTCGCGG AAGAGCCACC TGATTTTCCC CCAGCCCAAC TATGCGGACA 2401 CGCTCATCAG CCAGGAGAGC TGTGAGAAAA AGGATTTTTT ATCAGCGCCT CAATCTCTAC 2461 TCGAAGAAGA AAGAGAAGAA ACGTTTTCTC AGGTAATCTA TCTTTTCACA ACATACGTAC 2521 TAGCTAGTTT GCTGAAGTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 2581 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 2641 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGGCAGGC GACAGGCAGG 2701 CTTGTAGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt