Construct: ORF TRCN0000475762

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012983.1_s317c1
Derived from:
ccsbBroadEn_10092
DNA Barcode:
TTCATCTACACAGTTCCCTTGTCC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SCIMP (388325)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475762
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 388325 SCIMP SLP adaptor and CSK interac... NM_207103.3 99.7% 100% 300A>G
2 human 388325 SCIMP SLP adaptor and CSK interac... NM_001271842.1 94.9% 95.1% 124_125ins21;279A>G
3 human 388325 SCIMP SLP adaptor and CSK interac... NM_001319190.1 66.8% 54.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
504
ORF length:
435
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggatactttc acagttcagg attccactgc aatgagctgg tggaggaata
121atttctggat catcttagct gtggccatca tcgttgtctc tgtgggtctg ggcctcatcc
181tgtactgtgt ctgtaagtgg cagcttagac gaggcaagaa atgggaaatt gccaagcccc
241TGAAACACAA GCAAGTAGAT GAAGAAAAGA TGTATGAGAA TGTTCTTAAT GAGTCGCCAG
301TTCAATTACC GCCTCTGCCA CCGAGGAATT GGCCTTCTCT AGAAGACTCT TCCCCACAGG
361AAGCCCCGAG TCAGCCGCCC GCTACATACT CACTGGTAAA TAAAGTTAAA AATAAGAAGA
421CTGTTTCCAT CCCAAGCTAC ATTGAGCCTG AAGATGACTA TGACGATGTT GAAATCCCTG
481CAAATACTGA AAAAGCATCA TTTTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA
541AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC
601TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT TCATCTACAC
661AGTTCCCTTG TCCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag
721att

Download FASTA (ORF) (Full)