Construct: ORF TRCN0000475779
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009596.1_s317c1
- Derived from:
- BRDN0000391367
- DNA Barcode:
- GATACACTCTATATATTGTGTACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FYCO1 (79443)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475779
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79443 | FYCO1 | FYVE and coiled-coil domain... | NM_024513.4 | 99.7% | 99.7% | (many diffs) |
| 2 | human | 79443 | FYCO1 | FYVE and coiled-coil domain... | XM_006713333.3 | 99.7% | 99.7% | (many diffs) |
| 3 | human | 79443 | FYCO1 | FYVE and coiled-coil domain... | XM_006713334.3 | 99.7% | 99.7% | (many diffs) |
| 4 | human | 79443 | FYCO1 | FYVE and coiled-coil domain... | XM_011534111.3 | 99.7% | 99.7% | (many diffs) |
| 5 | human | 79443 | FYCO1 | FYVE and coiled-coil domain... | XR_001740265.1 | 88.6% | (many diffs) | |
| 6 | human | 79443 | FYCO1 | FYVE and coiled-coil domain... | XR_245157.1 | 54.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 4503
- ORF length:
- 4434
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcctccacc aatgcaggnn ntcagctcca gagaatcatc cgagacttgc 121 aagatgctgt gacagaacta agcaaagaat ttcaggaagc aggggaaccc atcacggatg 181 acagcaccag cttgcataaa ttttcttata aacttgagta tctcctgcaa tttgatcaga 241 aagagaaggc caccctcctg ggcaacaaga aggactactg ggattacttc tgtgcctgcc 301 tggccaaggt gaaaggagcc aatgatggga tccgctttgt caagtctatc tcagagctcc 361 gaacatcctt ggggaaagga agagcattta ttcgctactc cttggtgcac cagaggttgg 421 cagacacctt acagcagtgc ttcatgaaca ccaaagtgac cagtgactgg tactatgcaa 481 gaagcccctt tctgcagcca aagctgagct cggacattgt gggccaactc tatgagctga 541 ctgaggttca gtttgacctg gcgtcgaggg gctttgactt ggatgctgcc tggccaacat 601 ttgccaggag gacgctgacc actggctctt ctgcttacct gtggaaaccc cctagccgca 661 gctccagcat gagcagcttg gtgagcagct acctgcagac tcaagagatg gtgtccaact 721 ttgacctgaa cagcccccta aacaacgagg cattggaggg ctttgatgag atgcgactag 781 agctggacca gttggaggtg cgggagaagc agctacggga gcgcatgcag cagctggaca 841 gagagaacca ggagctgagg gcagctgtca gccagcaagg ggagcaactg cagacagaga 901 gggagagggg gcgcactgca gcggaggaca acgttcgcct cacttgcttg gtagctgagc 961 tccagaagca gtgggaggtc acccaggcca cccagaacac tgtgaaggag ctgcagacat 1021 gcctgcaggg cctggagcta ggagcagcag agaaggagga ggactaccac acagccctgc 1081 ggcggctgga gtccatgctg cagcccttgg cacaggagct tgaggccaca cgggactcac 1141 tggacaagaa aaaccagcat ttagccagct tcccaggctg gctagccatg gctcagcaga 1201 aggcagatac ggcatcagac acaaagggcc ggcaagaacc tattcccagt gatgcggccc 1261 aggagatgca ggagctaggg gagaagcttc aagccctaga aagggagaga accaaggtcg 1321 aggaggtcaa cagacagcag agtgcccaac tggaacagct ggtcaaggag cttcagctga 1381 aagaggatgc ccgggccagc ctggagcgcc tggtgaagga gatggcccca ctccaggagg 1441 agttgtctgg gaagggacag gaggcagacc agctctggcg acggctgcag gagttgctgg 1501 cccacacgag ctcctgggag gaggagctag cagagttgag gcgggagaaa aaacagcaac 1561 aggaggagaa ggagctgctg gagcaggagg tcaggtctct gacccggcag ctgcagttcc 1621 tggagaccca gctggcacag gtgagccaac atgtgagtga cctggaggag cagaagaagc 1681 agctcattca ggacaaagac cacctcagcc agcaggtggg tatgctcgag cggcttgctg 1741 ggccgcctgg cccagaactg ccagtggcag gtgagaagaa tgaggccctg gtccctgtga 1801 actccagtct gcaagaggcc tgggggaagc cagaggagga gcagaggggc ctgcaggagg 1861 cacagttaga cgataccaag gtgcaagagg gcagccagga ggaagagctc cggcaggcca 1921 acagggagct ggagaaggag ctacagaatg tggtcgggcg taaccagctc ctggagggca 1981 agctgcaagc cctgcaggcc gattaccagg ctttgcagca gcgggaatca gccatccagg 2041 gctccttggc ctccctggag gccgagcagg ccagcatccg gcacttgggt gaccagatgg 2101 aggcgagctt gctggctgta aggaaggcca aggaggccat gaaagcccag atggcagaga 2161 aggaggccat tctacagagc aaggagggcg agtgtcagca gctgcgggag gaggtggagc 2221 agtgccagca actggcagaa gcccggcaca gagagcttag ggctctcgag agccagtgcc 2281 agcagcagac ccagctgatt gaggtcctca cagcagagaa aggccaacag ggagttggcc 2341 cacccactga caatgaagcc cgtgagctgg ctgcccagct agccctgtct caggcgcagc 2401 tggaagtcca tcagggggag gtccaacggc tgcaggctca ggtggtggac ctccaggcca 2461 agatgcgggc agccctggat gaccaggaca aggtgcagag ccagctaagc atggctgagg 2521 ccgtcctgag ggagcacaaa acccttgtgc agcagctgaa ggagcagaat gaagccctta 2581 acagagccca tgtccaggag ctgctgcaat gctcggagcg tgaaggggca ctgcaggagg 2641 agagggccga tgaggcccag cagagggagg aggagctgcg ggccctgcag gaggagctgt 2701 cccaggccaa atgcagctcc gaggaagcac agctggagca cgctgagctg caagagcagc 2761 tgcaccgggc caacacagac acagctgagc tgggcatcca ggtttgcgca ctgaccgtgg 2821 aaaaggagcg agtggaggag gcactggcct gtgctgtcca ggagctccag gacgccaaag 2881 aggcagcctc aagggagcga gagggcctgg agcgccaagt agctgggctg cagcaagaga 2941 aggagagctt gcaggagaag ctgaaggcgg ccaaggcagc agccggctca ctgcctggcc 3001 tgcaggccca gctcgcccag gcagagcagc gggcccagag cctccaagag gctgcacacc 3061 aggagctcaa caccctcaag ttccagctga gtgctgaaat catggactac cagagcagac 3121 ttaagaatgc tggtgaagag tgcaagagcc tcaggggcca gcttgaggag caaggccggc 3181 agctgcaggc tgctgaggaa gctgtggaga agctgaaggc cacccaagca gacatgggag 3241 agaagctgag ctgcactagc aaccatcttg cagagtgcca ggcggccatg ctgaggaagg 3301 acaaggaggg ggctgccctg cgtgaagacc tagaaaggac ccagaaggaa ctcgaaaaag 3361 ccacaacaaa aatccaagag tattacaaca aactctgcca ggaggtgaca aatcgtgaga 3421 ggaatgacca gaagatgctt gctgacctgg atgacctcaa cagaaccaag aagtatctcg 3481 aggagcggct gatagagctg ctcagggaca aggatgctct ctggcagaag tcagatgccc 3541 tggaattcca gcagaagctc agtgctgagg agagatggct cggagacaca gaggcaaacc 3601 actgcctcga ctgtaagcgg gagttcagct ggatggtgcg cctccaccaa tgcaggatat 3661 gtggccgcat cttctgttac tactgctgca acaactacgt cctgagcaag cacggtggca 3721 aaaaggagcg ctgctgccga gcctgtttcc agaagctcag tgaaggccct ggctcccctg 3781 atagcagtgg ctcaggcact agccagggag agcccagccc tgcactgtca ccagcctcac 3841 ctgggcccca ggccacagga ggccaaggag caaatacaga ctacaggcca ccggacgacg 3901 ctgtgtttga tatcatcaca gatgaggaat tgtgccagat acaggagtcc ggctcctctt 3961 tgcctgaaac acccactgaa actgattctc tcgacccaaa tgcggctgaa caggatacta 4021 catcaacctc gctaacgcct gaggacactg aagacatgcc cgtggggcag gattcggaaa 4081 tctgcctgct gaagtctgga gaactgatga tcaaagtacc cctcacagtg gatgagatcg 4141 ccagcttcgg ggagggtagc agggagctgt ttgtgaggtc cagcacctac agcctgatcc 4201 ccatcactgt ggccgaggca ggcctcacca tcagctgggT CTTCTCCTCT GACCCCAAGA 4261 GCATCTCCTT CAGTGTGGTC TTCCAGGAGG CCGAGGACAC ACCGCTGGAT CAGTGTAAGG 4321 TCCTCATTCC CACGACCCGA TGCAACTCCC ACAAGGAGAA CATCCAGGGC CAGCTCAAGG 4381 TTCGCACACC CGGCATCTAC ATGCTCATCT TCGACAATAC CTTCTCAAGG TTTGTCTCTA 4441 AAAAGGTATT TTATCACTTG ACGGTTGATC GGCCTGTGAT CTACGATGGA AGTGATTTCC 4501 TGTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 4561 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 4621 GGCTTTATAT ATCTTGTGGA AAGGACGAGA TACACTCTAT ATATTGTGTA CAACGCGTTA 4681 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt