Transcript: Human XR_245157.1

PREDICTED: Homo sapiens FYVE and coiled-coil domain containing 1 (FYCO1), transcript variant X4, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
FYCO1 (79443)
Length:
8167
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_245157.1
NBCI Gene record:
FYCO1 (79443)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_245157.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000417889 GGACTACCAGAGCAGACTTAA pLKO_005 3251 3UTR 100% 13.200 10.560 N FYCO1 n/a
2 TRCN0000415599 GATCTACGATGGAAGTGATTT pLKO_005 4625 3UTR 100% 13.200 9.240 N FYCO1 n/a
3 TRCN0000038918 CCCTGCGTGAAGACCTAGAAA pLKO.1 3463 3UTR 100% 5.625 3.938 N FYCO1 n/a
4 TRCN0000038917 CGGGACTCACTGGACAAGAAA pLKO.1 1278 3UTR 100% 5.625 3.938 N FYCO1 n/a
5 TRCN0000038914 CCAGAGAATCATCCGAGACTT pLKO.1 245 3UTR 100% 4.950 3.465 N FYCO1 n/a
6 TRCN0000038915 GCTGTGACAGAACTAAGCAAA pLKO.1 273 3UTR 100% 4.950 3.465 N FYCO1 n/a
7 TRCN0000038916 CATCTCCTTCAGTGTGGTCTT pLKO.1 4409 3UTR 100% 4.050 2.835 N FYCO1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_245157.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000475779 GATACACTCTATATATTGTGTACA pLX_317 6.1% 54.1% V5 (many diffs) n/a
2 ccsbBroadEn_12566 pDONR223 100% 9.2% None (many diffs) n/a
3 ccsbBroad304_12566 pLX_304 0% 9.2% V5 (many diffs) n/a
4 TRCN0000491775 ATTCCACATCAAACTATGGTGGTC pLX_317 52.9% 9.2% V5 (many diffs) n/a
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