Transcript: Human XM_006713333.3

PREDICTED: Homo sapiens FYVE and coiled-coil domain containing 1 (FYCO1), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
FYCO1 (79443)
Length:
9679
CDS:
1381..5817

Additional Resources:

NCBI RefSeq record:
XM_006713333.3
NBCI Gene record:
FYCO1 (79443)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_006713333.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000417889 GGACTACCAGAGCAGACTTAA pLKO_005 4416 CDS 100% 13.200 10.560 N FYCO1 n/a
2 TRCN0000415599 GATCTACGATGGAAGTGATTT pLKO_005 5790 CDS 100% 13.200 9.240 N FYCO1 n/a
3 TRCN0000038918 CCCTGCGTGAAGACCTAGAAA pLKO.1 4628 CDS 100% 5.625 3.938 N FYCO1 n/a
4 TRCN0000038917 CGGGACTCACTGGACAAGAAA pLKO.1 2443 CDS 100% 5.625 3.938 N FYCO1 n/a
5 TRCN0000038914 CCAGAGAATCATCCGAGACTT pLKO.1 1410 CDS 100% 4.950 3.465 N FYCO1 n/a
6 TRCN0000038915 GCTGTGACAGAACTAAGCAAA pLKO.1 1438 CDS 100% 4.950 3.465 N FYCO1 n/a
7 TRCN0000038916 CATCTCCTTCAGTGTGGTCTT pLKO.1 5574 CDS 100% 4.050 2.835 N FYCO1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006713333.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000475779 GATACACTCTATATATTGTGTACA pLX_317 6.1% 99.7% 99.7% V5 (many diffs) n/a
2 ccsbBroadEn_12566 pDONR223 100% 17% 16.8% None (many diffs) n/a
3 ccsbBroad304_12566 pLX_304 0% 17% 16.8% V5 (many diffs) n/a
4 TRCN0000491775 ATTCCACATCAAACTATGGTGGTC pLX_317 52.9% 17% 16.8% V5 (many diffs) n/a
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