Construct: ORF TRCN0000475781
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007248.1_s317c1
- Derived from:
- ccsbBroadEn_13235
- DNA Barcode:
- ATTGCCAAAGTATATTTTTTTACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UNC45B (146862)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475781
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 146862 | UNC45B | unc-45 myosin chaperone B | NM_001308281.1 | 99.9% | 100% | 381G>A |
| 2 | human | 146862 | UNC45B | unc-45 myosin chaperone B | XM_017024234.1 | 99.9% | 100% | 381G>A |
| 3 | human | 146862 | UNC45B | unc-45 myosin chaperone B | NM_001033576.2 | 91.4% | 91.4% | 381G>A;1452_1688del |
| 4 | human | 146862 | UNC45B | unc-45 myosin chaperone B | NM_001267052.2 | 91.4% | 91.4% | 381G>A;1452_1688del |
| 5 | human | 146862 | UNC45B | unc-45 myosin chaperone B | NM_173167.3 | 91.2% | 91.2% | 381G>A;1453_1695del |
| 6 | mouse | 217012 | Unc45b | unc-45 myosin chaperone B | NM_178680.4 | 81.2% | 86.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2619
- ORF length:
- 2550
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcagaggtg gaagcggtac agctgaagga ggaaggaaac cggcatttcc 121 agctccagga ctacaaggcc gccacaaata gctacagcca ggccctgaag ctgaccaagg 181 acaaggccct gctggccacg ctttatcgga accgggcagc ctgtggcctg aaaacggaga 241 gctacgtcca ggcagcttca gatgcctcca gagccatcga catcaactcc tcggacatca 301 aggctctgta tcggcgatgc caggcactgg agcacctggg gaagctggac caggccttca 361 aagacgtgca gcgttgtgcc accctcgagc cacggaacca gaacttccag gagatgctga 421 ggagactcaa caccagcatt caggagaaac tccgagtgca gttctccaca gactcgaggg 481 tacagaagat gtttgagatc ctcttggatg aaaacagtga ggctgataag cgggaaaagg 541 ctgccaacaa tctcattgtc ctaggccgtg aggaagcagg ggctgagaag atcttccaga 601 acaatggagt agccttgcta ctgcagcttc tggacactaa gaagcctgag ctggtgctgg 661 ctgcagtgcg gaccctgtcg ggcatgtgca gcggccacca agccagagcc acagtgattc 721 tgcatgcagt gcggatagac cgaatctgta gcctcatggc cgtggagaat gaggagatgt 781 ctctggctgt ctgcaacctg ctccaagcca tcattgactc cttgtctggg gaggacaagc 841 gggagcatcg agggaaggag gaggccctgg ttctagacac caagaaggac ctgaagcaga 901 tcaccagcca cctgctggac atgctagtca gcaagaaggt gtctggccag ggcagggatc 961 aggcgctgaa cctgctcaat aagaatgttc ccaggaagga ccttgccatt catgacaact 1021 cacgtaccat ctatgtggtg gataatggtc tgaggaagat cctgaaggtt gtggggcagg 1081 ttccagatct gccatcctgc ctgcccctga ctgacaacac ccgcatgctg gcctctatcc 1141 tcatcaacaa gctctatgat gacctgcgct gtgacccgga gcgcgatcac ttccgcaaga 1201 tctgtgagga atatatcacg ggcaagtttg acccccagga catggacaag aacttgaatg 1261 ccatccagac agtgtcaggg atcctgcagg gcccctttga cctgggcaac cagctgctgg 1321 gactgaaagg tgtgatggag atgatggtgg cactatgtgg ctcagagcgc gagacggacc 1381 agctggtggc cgtggaggcc ctcatccatg cctccacgaa gctcagccgc gccaccttca 1441 tcatcaccaa tggagtgtca ctgctcaaac agatctacaa gaccaccaaa aatgagaaga 1501 tcaagatccg cacactggtg accagtgaca agaccatcct gtactcggtg gccaccaccc 1561 tggtgaactg caccaacagc tacgatgtca aggaggtcat cccagagctt gtccagctcg 1621 ccaagttctc caagcagcat gtgcccgagg aacaccccaa ggacaagaag gactttatag 1681 acatgcgggt gaagcggctt ctgaaggcgg gtgtcatctc tgccctggct tgcatggtga 1741 aagcagatag tgccatcctc actgaccaga ccaaggagct gctggccagg gtattcctgg 1801 cactgtgtga caacccaaag gaccgaggca ccattgtggc tcaaggtggt ggcaaggccc 1861 tgattcccct ggctttggag ggcacagatg tgggcaaggt gaaggcagcc cacgctctag 1921 caaagatcgc tgctgtctcc aatccggaca ttgcttttcc tggggagcgg gtgtatgagg 1981 tggtgcggcc ccttgtaaga ctcttggaca cacagaggga tgggcttcag aactatgagg 2041 ctctcctagg cctcaccaac ctgtctgggc ggagtgacaa actccggcag aagatcttta 2101 aggagagggc cttgccagac atcgagaact acatgtttga gaatcatgat cagctgcggc 2161 aggcggccac cgagtgcatg tgcaacatgg tgctccacaa ggaggtacag gaaaggttct 2221 tggctgacgg gaatgaccgg ctgaagctgg tggtgctgcT CTGCGGGGAG GATGATGATA 2281 AGGTGCAGAA TGCGGCTGCA GGGGCTCTGG CCATGCTGAC AGCAGCACAC AAGAAACTGT 2341 GCCTCAAGAT GACTCAAGTG ACAACCCAGT GGTTGGAGAT CCTCCAGCGG CTTTGCCTGC 2401 ACGACCAGCT GTCTGTCCAA CACCGGGGCC TGGTCATTGC CTACAACCTA CTGGCAGCCG 2461 ATGCTGAGCT GGCCAAGAAG CTGGTGGAGA GTGAGCTGCT GGAGATCCTG ACTGTGGTGG 2521 GCAAACAGGA GCCAGATGAG AAGAAGGCAG AAGTGGTTCA GACAGCCCGA GAATGTCTCA 2581 TCAAGTGCAT GGATTATGGT TTCATTAAAC CAGTGTCTTT GCCAACTTTC TTGTACAAAG 2641 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 2701 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 2761 ACGAATTGCC AAAGTATATT TTTTTACCAC GCGTTAAGTC gacaatcaac ctctggatta 2821 caaaatttgt gaaagatt