Construct: ORF TRCN0000475797
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002957.1_s317c1
- Derived from:
- ccsbBroadEn_05469
- DNA Barcode:
- TTACTGGTTTAACGATTTGGTCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CLPSL1 (340204)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475797
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 340204 | CLPSL1 | colipase like 1 | NM_001010886.5 | 100% | 100% | |
| 2 | human | 340204 | CLPSL1 | colipase like 1 | XM_017010820.1 | 70.9% | 64.7% | (many diffs) |
| 3 | human | 340204 | CLPSL1 | colipase like 1 | NM_001348773.2 | 69.8% | 63.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 432
- ORF length:
- 363
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gatgctaccc caatggctgc tgctgctgtt ccttctcttc ttctttctct 121 tcctcctcac caggggctca ctttctccaa caaaatacaa ccttttggag ctcaaggagt 181 cttgcatccg gaaccaggac tgcgagactg gctgctgcca acgtgcTCCA GACAATTGCG 241 AGTCGCACTG CGCGGAGAAG GGGTCCGAGG GCAGTCTGTG TCAAACGCAG GTGTTCTTTG 301 GCCAATATAG AGCGTGTCCC TGCCTGCGGA ACCTGACTTG TATATATTCA AAGAATGAGA 361 AATGGCTTAG CATCGCCTAT GGCCGTTGTC AGAAAATTGG AAGGCAGAAG TTGGCTAAGA 421 AAATGTTCTT CTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 481 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 541 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATTA CTGGTTTAAC GATTTGGTCT 601 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t