Construct: ORF TRCN0000475809
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008516.1_s317c1
- Derived from:
- ccsbBroadEn_14042
- DNA Barcode:
- GGGATCCATAGTTAGTCTAAAATC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- CWC27 (10283)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475809
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10283 | CWC27 | CWC27 spliceosome associate... | NM_001297644.1 | 74.3% | 2.5% | 1_300del;306delA |
| 2 | human | 10283 | CWC27 | CWC27 spliceosome associate... | XM_011543095.3 | 65.3% | 2.7% | (many diffs) |
| 3 | human | 10283 | CWC27 | CWC27 spliceosome associate... | NM_001364478.1 | 64.4% | 1.9% | (many diffs) |
| 4 | human | 10283 | CWC27 | CWC27 spliceosome associate... | NM_005869.4 | 61.5% | 2% | (many diffs) |
| 5 | human | 10283 | CWC27 | CWC27 spliceosome associate... | NM_001297645.1 | 60.3% | 1.5% | (many diffs) |
| 6 | human | 10283 | CWC27 | CWC27 spliceosome associate... | XM_011543096.2 | 60% | 2.8% | (many diffs) |
| 7 | human | 10283 | CWC27 | CWC27 spliceosome associate... | NM_001318000.1 | 55.5% | 2.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 204
- ORF length:
- 138
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc aatgctggtt ctcatgataa tggcagccag tttttcttca cactgggtcg 121 agcagatgaa cttaacaata agcataccat ctttggaaag gttacagggg atacagtata 181 taacatgttg cgactgtcag aagtagacat tgatgatgac gaaagaccac ataatccaca 241 caaaataaaa agctgtgagg ttttgtttaa tccttttgat gacatcattc caagggaaat 301 taaaaggctg aaaaaagaga aaccagagga ggaagtaaag aaattgaaac ccaaaggcac 361 aaaaaatttt agtttacttt catttggaga ggaagctgag gaagaagagg aggaagtaaa 421 tcgagttagt cagagcatga agggcaaaag caaaagtagt catgacttgc ttaaggatga 481 tccacatctc agttctgttc cagttgtaga aagtgaaaaa ggtgatgcac cagatttagt 541 tgatgatgga gaagatgaaa gtgcagagca tgatgaatat attgatggtg atgaaaagaa 601 ccTGATGAGA GAAAGAATTG CCAAAAAATT AAAAAAGGAC ACAAGTGCGA ATGTTAAATC 661 AGCTGGAGAA GGAGAAGTGG AGAAGAAATC AGTCAGCCGC AGTGAAGAGC TCAGAAAAGA 721 AGCAAGACAA TTAAAACGGG AACTCTTAGC AGCAAAACAA AAAAAAGTAG AAAATGCAGC 781 AAAACAAGCA GAAAAAAGAA GTGAAGAGGA AGAAGCCCCT CCAGATGGTG CTGTTGCCGA 841 ATACAGAAGA GAAAAGCAAA AGTATGAAGC TTTGAGGAAG CAACAGTCAA AGAAGGGAAC 901 TTCCCGGGAA GATCAGGATG TCACATGTAC TTCAGTTTGC CCAACTTTCT TGTACAAAGT 961 GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG 1021 AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA 1081 CGAGGGATCC ATAGTTAGTC TAAAATCACG CGTTAAGTCg acaatcaacc tctggattac 1141 aaaatttgtg aaagatt