Construct: ORF TRCN0000475816
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005455.1_s317c1
- Derived from:
- ccsbBroadEn_01711
- DNA Barcode:
- CGCACAGCGTTGAAACACGAAACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRHR (7201)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475816
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7201 | TRHR | thyrotropin releasing hormo... | NM_003301.6 | 100% | 100% | |
| 2 | mouse | 22045 | Trhr | thyrotropin releasing hormo... | XM_006520747.1 | 90.2% | 94.4% | (many diffs) |
| 3 | mouse | 22045 | Trhr | thyrotropin releasing hormo... | NM_013696.2 | 89.2% | 93.2% | (many diffs) |
| 4 | mouse | 22045 | Trhr | thyrotropin releasing hormo... | XM_006520748.2 | 59.7% | 62% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1263
- ORF length:
- 1194
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaaaacgag acagtcagtg aactgaacca aacacagctt cagccacgag 121 cagtggtggc cttagaatac caggtggtca ccatcttact tgtactcatt atttgtggcc 181 tgggcattgt aggcaacatc atggtagtcc tggttgtcat gagaaccaag cacatgagga 241 cccccacaaa ctgctacctg gtgagcctgg cagtagctga tctcatggtc ttggtggccg 301 caggcctccc caacataaca gacagtatct acggttcctg ggtctatggc tatgttggat 361 gcctctgcat tacttacctc cagtatttgg gaattaatgc atcctcttgt tcaataacag 421 cctttaccat tgagaggtac atagcaatct gtcaccccat caaagcccag tttctctgca 481 cattttccag agccaaaaag attatcatct ttgtctgggc tttcacatct ctttactgta 541 tgctctggtt cttcttgctg gatctcaata ttagcaccta caaagatgct attgtgatat 601 cctgtggcta caagatctcc aggaattact actcacctat ttacctaatg gactttggtg 661 tcttttatgt tgtgccaatg atcctggcta ccgtcctcta tggattcata gctagaatcc 721 ttttcttaaa tcccattcct tcagatccta aagaaaactc taagacatgg aaaaatgatt 781 caacccatca gaacacaaat ctgaatgtaa atacctctaa tagatgtttc aacagcacag 841 tatcttcaag gaagcaggtc accaagatgc tggcagtggt tgtaattctg tttgcccttt 901 tatggatgcc ctacaggact ctagtgGTTG TCAACTCATT TCTCTCCAGT CCTTTCCAAG 961 AAAATTGGTT TTTGCTCTTT TGCAGAATTT GCATTTATCT CAACAGTGCC ATCAACCCGG 1021 TGATTTACAA TCTCATGTCC CAGAAATTCC GTGCAGCCTT CAGAAAGCTC TGCAACTGCA 1081 AGCAGAAGCC AACAGAGAAA CCTGCTAACT ACAGTGTGGC CCTAAATTAC AGCGTCATCA 1141 AGGAGTCAGA CCATTTCAGC ACAGAGCTTG ATGATATCAC TGTCACTGAC ACTTACCTGT 1201 CTGCCACAAA AGTGTCTTTT GATGACACCT GCTTGGCTTC TGAGGTATCC TTTAGCCAAA 1261 GTTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1321 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1381 GGCTTTATAT ATCTTGTGGA AAGGACGACG CACAGCGTTG AAACACGAAA CAACGCGTTA 1441 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt