Construct: ORF TRCN0000475824
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006524.1_s317c1
- Derived from:
- ccsbBroadEn_02142
- DNA Barcode:
- TCTAGGGCTACTAGATCGGGCCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- B4GALT5 (9334)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475824
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9334 | B4GALT5 | beta-1,4-galactosyltransfer... | NM_004776.4 | 100% | 100% | |
| 2 | mouse | 56336 | B4galt5 | UDP-Gal:betaGlcNAc beta 1,4... | NM_019835.2 | 87.4% | 95.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1233
- ORF length:
- 1164
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcgcgcccgc cgggggctgc tgcggctgcc gcgccgctcg ctgctcgccg 121 cgctcttctt cttttctctc tcgtcctcgc tgctgtactt cgtctatgtg gcgcccggca 181 tagtgaacac ctacctcttc atgatgcaag cccaaggcat tctgatccgg gacaacgtga 241 gaacaatcgg tgctcaggtt tatgagcagg tgcttcggag tgcttatgcc aagaggaaca 301 gcagtgtaaa tgactcagat tatcctcttg acttgaacca cagtgaaacc ttcctgcaaa 361 ctacaacatt tcttcctgaa gacttcacct actttgcaaa ccatacctgc cctgaaagac 421 tcccttccat gaagggccca atagacataa acatgagtga aattggaatg gattacattc 481 atgaactctt ctccaaagac ccaaccatca agctcggagg tcactggaag ccttctgatt 541 gcatgcctcg gtggaaggtg gcgatcctta tccccttccg gaaccgccac gagcacctcc 601 cagtcctgtt cagacacctg cttcccatgc tccagcgcca gcgcttgcag tttgcatttt 661 atgtggttga acaagttggt acccaaccct ttaatcgagc catgcttttc aacgttggct 721 ttcaagaggc aatgaaagac ttggattggg actgtttgat ttttcatgat gtagatcaca 781 taccggaaag tgatcgcaac tattatggat gtggacagat gccgaggcat tttgcaacca 841 aattggataa gtatatgtat ctgcttcctt ataccgagtt ctttGGCGGA GTGAGTGGCT 901 TAACAGTGGA ACAATTTCGG AAAATCAATG GCTTTCCTAA TGCTTTCTGG GGTTGGGGTG 961 GAGAAGATGA CGACCTCTGG AACAGAGTAC AGAATGCAGG CTATTCTGTG AGCCGGCCAG 1021 AGGGTGACAC AGGAAAGTAC AAGTCCATTC CTCATCACCA TCGAGGAGAA GTCCAGTTTC 1081 TTGGAAGGTA TGCTCTGCTG AGGAAGTCAA AAGAACGGCA AGGGCTGGAT GGCCTCAACA 1141 ACCTGAACTA CTTTGCAAAC ATCACATACG ACGCCTTGTA TAAAAACATA ACTGTCAACC 1201 TGACACCCGA GCTGGCTCAG GTGAACGAGT ACTTGCCAAC TTTCTTGTAC AAAGTGGTTG 1261 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1321 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATC 1381 TAGGGCTACT AGATCGGGCC GGACGCGTTA AGTCgacaat caacctctgg attacaaaat 1441 ttgtgaaaga tt