Transcript: Mouse NM_019835.2

Mus musculus UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 5 (B4galt5), mRNA.

Source:
NCBI, updated 2017-05-14
Taxon:
Mus musculus (mouse)
Gene:
B4galt5 (56336)
Length:
4205
CDS:
147..1313

Additional Resources:

NCBI RefSeq record:
NM_019835.2
NBCI Gene record:
B4galt5 (56336)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_019835.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000018786 CAACCTGAACTACTCTGCGAA pLKO.1 1217 CDS 100% 2.640 3.696 N B4galt5 n/a
2 TRCN0000018784 CATACCTGAGAGTGACCGAAA pLKO.1 857 CDS 100% 4.050 2.835 N B4galt5 n/a
3 TRCN0000018785 CATAGTGAACACCTACCTCTT pLKO.1 257 CDS 100% 4.050 2.835 N B4galt5 n/a
4 TRCN0000018783 GCCTTCTATGTGATCGAGCAA pLKO.1 732 CDS 100% 2.640 1.848 N B4galt5 n/a
5 TRCN0000018782 GCAGCCTGAATGACTCAGATT pLKO.1 379 CDS 100% 4.950 2.970 N B4galt5 n/a
6 TRCN0000035363 CCTCAACAACCTGAACTACTT pLKO.1 1211 CDS 100% 4.950 3.465 N B4GALT5 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_019835.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02142 pDONR223 100% 87.4% 95.1% None (many diffs) n/a
2 ccsbBroad304_02142 pLX_304 0% 87.4% 95.1% V5 (many diffs) n/a
3 TRCN0000475824 TCTAGGGCTACTAGATCGGGCCGG pLX_317 30.2% 87.4% 95.1% V5 (many diffs) n/a
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