Construct: ORF TRCN0000475825
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000983.1_s317c1
- Derived from:
- ccsbBroadEn_05841
- DNA Barcode:
- TATTCACATATGCACATGCTATTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AQP1 (358)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475825
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 358 | AQP1 | aquaporin 1 (Colton blood g... | NM_198098.3 | 99.8% | 99.6% | 133G>A |
| 2 | human | 358 | AQP1 | aquaporin 1 (Colton blood g... | NM_001329872.2 | 82.5% | 81.4% | (many diffs) |
| 3 | mouse | 11826 | Aqp1 | aquaporin 1 | NM_007472.2 | 87.9% | 94% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 873
- ORF length:
- 807
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cagcgagttc aagaagaagc tcttctggag ggcagtggtg gccgagttcc 121 tggccacgac cctctttgtc ttcatcagca tcggttctgc cctgggcttc aaatacccgg 181 tggggaacaa ccagacgacg gtccaggaca acgtgaaggt gtcgctggcc ttcgggctga 241 gcatcgccac gctggcgcag agtgtgggcc acatcagcgg cgcccacctc aacccggctg 301 tcacactggg gctgctgctc agctgccaga tcagcatctt ccgtgccctc atgtacatca 361 tcgcccagtg cgtgggggcc atcgtcgcca ccgccatcct ctcaggcatc acctcctccc 421 tgactgggaa ctcgcttggc cgcaatgacc tggctgatgg tgtgaactcg ggccagggcc 481 tgggcatcga gatcatcggg accctccagc tggtgctatg cgtgctggct actaccgacc 541 ggaggcgccg tgaccttggt ggcTCAGCCC CCCTTGCCAT CGGCCTCTCT GTAGCCCTTG 601 GACACCTCCT GGCTATTGAC TACACTGGCT GTGGGATTAA CCCTGCTCGG TCCTTTGGCT 661 CCGCGGTGAT CACACACAAC TTCAGCAACC ACTGGATTTT CTGGGTGGGG CCATTCATCG 721 GGGGAGCCCT GGCTGTACTC ATCTACGACT TCATCCTGGC CCCACGCAGC AGTGACCTCA 781 CAGACCGCGT GAAGGTGTGG ACCAGCGGCC AGGTGGAGGA GTATGACCTG GATGCCGACG 841 ACATCAACTC CAGGGTGGAG ATGAAGCCCA AATACCCAAC TTTCTTGTAC AAAGTGGTTG 901 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 961 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATA 1021 TTCACATATG CACATGCTAT TTACGCGTTA AGTCgacaat caacctctgg attacaaaat 1081 ttgtgaaaga tt