Construct: ORF TRCN0000475831

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004803.1_s317c1
Derived from:
ccsbBroadEn_06282
DNA Barcode:
AGCTGTTTTCGAAGCGAGCATGCA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GLDC (2731)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475831
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2731 GLDC glycine decarboxylase NM_000170.2 99.9% 100% 249G>A
2 mouse 104174 Gldc glycine decarboxylase NM_138595.2 86.4% 92% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
3129
ORF length:
3060
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gcagtcctgt gccagggcgt gggggctgcg cctgggccgc ggggtcgggg
121gcggccgccg cctggctggg ggatcggggc cgtgctgggc gccgcggagc cgggacagca
181gcagtggcgg cggggacagc gccgcggctg gggcctcgcg cctcctggag cgccttctgc
241ccagacacga cgacttcgct cggaggcaca tcggccctgg ggacaaagac cagagagaga
301tgctgcagac cttgggactg gcgagcattg atgaattgat cgagaagacg gtccctgcca
361acatccgttt gaaaagaccc ttgaaaatgg aagaccctgt ttgtgaaaat gaaatccttg
421caactctgca tgccatttca agcaaaaacc agatctggag atcgtatatt ggcatgggct
481attataactg ctcagtgcca cagacgattt tgcggaactt actggagaac tcaggatgga
541tcacccagta tactccatac cagcctgagg tgtctcaggg gaggctggag agtttactca
601actaccagac catggtgtgt gacatcacag gcctggacat ggccaatgca tccctgctgg
661atgaggggac tgcagccgca gaggcactgc agctgtgcta cagacacaac aagaggagga
721aatttctcgt tgatccccgt tgccacccac agacaatagc tgttgtccag actcgagcca
781aatatactgg agtcctcact gagctgaagt taccctgtga aatggacttc agtggaaaag
841atgtcagtgg agtgttgttc cagtacccag acacggaggg gaaggtggaa gactttacgg
901aactcgtgga gagagctcat cagagtggga gcctggcctg ctgtgctact gaccttttag
961ctttgtgcat cttgaggcca cctggagaat ttggggtaga catcgccctg ggcagctccc
1021agagatttgg agtgccactg ggctatgggg gaccccatgc agcatttttt gctgtccgag
1081aaagcttggt gagaatgatg cctggaagaa tggtgggggt aacaagagat gccactggga
1141aagaagtgta tcgtcttgct cttcaaacca gggagcaaca cattcggaga gacaaggcta
1201ccagcaacat ctgtacagct caggccctct tggcgaatat ggctgccatg tttgcaatct
1261accatggttc ccatgggctg gagcatattg ctaggagggt acataatgcc actttgattt
1321tgtcagaagg tctcaagcga gcagggcatc aactccagca tgacctgttc tttgatacct
1381tgaagattca gtgtggctgc tcagtgaagg aggtcttggg cagggccgct cagcggcaga
1441tcaattttcg gctttttgag gatggcacac ttggtatttc tcttgatgaa acagtcaatg
1501aaaaagatct ggacgatttg ttgtggatct ttggttgtga gtcatctgca gaactggttg
1561ctgaaagcat gggagaggag tgcagaggta ttccagggtc tgtgttcaag aggaccagcc
1621cgttcctcac ccatcaagtg ttcaacagct accactctga aacaaacatt gtccggtaca
1681tgaagaaact ggaaaataaa gacatttccc ttgttcacag catgattcca ctgggatcct
1741gcaccatgaa actgaacagt tcgtctgaac tcgcacctat cacatggaaa gaatttgcaa
1801acatccaccc ctttgtgcct ctggatcaag ctcaaggata tcagcagctt ttccgagagc
1861ttgagaagga tttgtgtgaa ctcacaggtt atgaccaggt ctgtttccag ccaaacagcg
1921gagcccaggg agaatatgct ggactggcca ctatccgagc ctacttaaac cagaaaggag
1981aggggcacag aacggtttgc ctcattccga aatcagcaca tgggaccaac ccagcaagtg
2041cccacatggc aggcatgaag attcagcctg tggaggtgga taaatatggg aatatcgatg
2101cagttcacct caaggccatg gtggataagc acaaggagaa cctagcagct atcatgatta
2161catacccatc caccaatggg gtgtttgaag agaacatcag tgacgtgtgt gacctcatcc
2221atcaacatgg aggacaggtc tacctagacg gggcaaatat gaatgctcag gtgggaatct
2281gtcgccctgg agacttcggg tctgatgtct cgcacctaaa tcttcacaag accttctgca
2341ttccccacgg aggaggtggt cctggcatgg ggcccatcgg agtgaagaaa catctcgccc
2401cgtttttgcc caatcatccc gtcatttcac taaagcggaa tgaggatgcc tgtcctgtgg
2461gaaccgtcag tgcggcccca tggggctcca gttccatctt gcccatttcc tgggcttata
2521tcaagatgat gggaggcaag ggtcttaaac aagccacgga aactgcgata ttaaatgcca
2581actacatggc caagcgatta gaaacacact acagaattct tttcaggggt gcaagaggtt
2641atgtgggtca tgaatttatt ttggacacga gacccttcaa aaagtctgca aatattgagg
2701ctgtggatgt ggccaagaga ctccaggatt atggatttca cgcccctacc atgtcctggc
2761ctgtggcagg gacccTCATG GTGGAGCCCA CTGAGTCGGA GGACAAGGCA GAGCTGGACA
2821GATTCTGTGA TGCCATGATC AGCATTCGGC AGGAAATTGC TGACATTGAG GAGGGCCGCA
2881TCGACCCCAG GGTCAATCCG CTGAAGATGT CTCCACACTC CCTGACCTGC GTTACATCTT
2941CCCACTGGGA CCGGCCTTAT TCCAGAGAGG TGGCAGCATT CCCACTCCCC TTCGTGAAAC
3001CAGAGAACAA ATTCTGGCCA ACGATTGCCC GGATTGATGA CATATATGGA GATCAGCACC
3061TGGTTTGTAC CTGCCCACCC ATGGAAGTTT ATGAGTCTCC ATTTTCTGAA CAAAAGAGGG
3121CGTCTTCTTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
3181CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
3241TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAGCTGT TTTCGAAGCG AGCATGCAAC
3301GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)