Construct: ORF TRCN0000475831
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004803.1_s317c1
- Derived from:
- ccsbBroadEn_06282
- DNA Barcode:
- AGCTGTTTTCGAAGCGAGCATGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GLDC (2731)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475831
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2731 | GLDC | glycine decarboxylase | NM_000170.2 | 99.9% | 100% | 249G>A |
| 2 | mouse | 104174 | Gldc | glycine decarboxylase | NM_138595.2 | 86.4% | 92% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3129
- ORF length:
- 3060
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcagtcctgt gccagggcgt gggggctgcg cctgggccgc ggggtcgggg 121 gcggccgccg cctggctggg ggatcggggc cgtgctgggc gccgcggagc cgggacagca 181 gcagtggcgg cggggacagc gccgcggctg gggcctcgcg cctcctggag cgccttctgc 241 ccagacacga cgacttcgct cggaggcaca tcggccctgg ggacaaagac cagagagaga 301 tgctgcagac cttgggactg gcgagcattg atgaattgat cgagaagacg gtccctgcca 361 acatccgttt gaaaagaccc ttgaaaatgg aagaccctgt ttgtgaaaat gaaatccttg 421 caactctgca tgccatttca agcaaaaacc agatctggag atcgtatatt ggcatgggct 481 attataactg ctcagtgcca cagacgattt tgcggaactt actggagaac tcaggatgga 541 tcacccagta tactccatac cagcctgagg tgtctcaggg gaggctggag agtttactca 601 actaccagac catggtgtgt gacatcacag gcctggacat ggccaatgca tccctgctgg 661 atgaggggac tgcagccgca gaggcactgc agctgtgcta cagacacaac aagaggagga 721 aatttctcgt tgatccccgt tgccacccac agacaatagc tgttgtccag actcgagcca 781 aatatactgg agtcctcact gagctgaagt taccctgtga aatggacttc agtggaaaag 841 atgtcagtgg agtgttgttc cagtacccag acacggaggg gaaggtggaa gactttacgg 901 aactcgtgga gagagctcat cagagtggga gcctggcctg ctgtgctact gaccttttag 961 ctttgtgcat cttgaggcca cctggagaat ttggggtaga catcgccctg ggcagctccc 1021 agagatttgg agtgccactg ggctatgggg gaccccatgc agcatttttt gctgtccgag 1081 aaagcttggt gagaatgatg cctggaagaa tggtgggggt aacaagagat gccactggga 1141 aagaagtgta tcgtcttgct cttcaaacca gggagcaaca cattcggaga gacaaggcta 1201 ccagcaacat ctgtacagct caggccctct tggcgaatat ggctgccatg tttgcaatct 1261 accatggttc ccatgggctg gagcatattg ctaggagggt acataatgcc actttgattt 1321 tgtcagaagg tctcaagcga gcagggcatc aactccagca tgacctgttc tttgatacct 1381 tgaagattca gtgtggctgc tcagtgaagg aggtcttggg cagggccgct cagcggcaga 1441 tcaattttcg gctttttgag gatggcacac ttggtatttc tcttgatgaa acagtcaatg 1501 aaaaagatct ggacgatttg ttgtggatct ttggttgtga gtcatctgca gaactggttg 1561 ctgaaagcat gggagaggag tgcagaggta ttccagggtc tgtgttcaag aggaccagcc 1621 cgttcctcac ccatcaagtg ttcaacagct accactctga aacaaacatt gtccggtaca 1681 tgaagaaact ggaaaataaa gacatttccc ttgttcacag catgattcca ctgggatcct 1741 gcaccatgaa actgaacagt tcgtctgaac tcgcacctat cacatggaaa gaatttgcaa 1801 acatccaccc ctttgtgcct ctggatcaag ctcaaggata tcagcagctt ttccgagagc 1861 ttgagaagga tttgtgtgaa ctcacaggtt atgaccaggt ctgtttccag ccaaacagcg 1921 gagcccaggg agaatatgct ggactggcca ctatccgagc ctacttaaac cagaaaggag 1981 aggggcacag aacggtttgc ctcattccga aatcagcaca tgggaccaac ccagcaagtg 2041 cccacatggc aggcatgaag attcagcctg tggaggtgga taaatatggg aatatcgatg 2101 cagttcacct caaggccatg gtggataagc acaaggagaa cctagcagct atcatgatta 2161 catacccatc caccaatggg gtgtttgaag agaacatcag tgacgtgtgt gacctcatcc 2221 atcaacatgg aggacaggtc tacctagacg gggcaaatat gaatgctcag gtgggaatct 2281 gtcgccctgg agacttcggg tctgatgtct cgcacctaaa tcttcacaag accttctgca 2341 ttccccacgg aggaggtggt cctggcatgg ggcccatcgg agtgaagaaa catctcgccc 2401 cgtttttgcc caatcatccc gtcatttcac taaagcggaa tgaggatgcc tgtcctgtgg 2461 gaaccgtcag tgcggcccca tggggctcca gttccatctt gcccatttcc tgggcttata 2521 tcaagatgat gggaggcaag ggtcttaaac aagccacgga aactgcgata ttaaatgcca 2581 actacatggc caagcgatta gaaacacact acagaattct tttcaggggt gcaagaggtt 2641 atgtgggtca tgaatttatt ttggacacga gacccttcaa aaagtctgca aatattgagg 2701 ctgtggatgt ggccaagaga ctccaggatt atggatttca cgcccctacc atgtcctggc 2761 ctgtggcagg gacccTCATG GTGGAGCCCA CTGAGTCGGA GGACAAGGCA GAGCTGGACA 2821 GATTCTGTGA TGCCATGATC AGCATTCGGC AGGAAATTGC TGACATTGAG GAGGGCCGCA 2881 TCGACCCCAG GGTCAATCCG CTGAAGATGT CTCCACACTC CCTGACCTGC GTTACATCTT 2941 CCCACTGGGA CCGGCCTTAT TCCAGAGAGG TGGCAGCATT CCCACTCCCC TTCGTGAAAC 3001 CAGAGAACAA ATTCTGGCCA ACGATTGCCC GGATTGATGA CATATATGGA GATCAGCACC 3061 TGGTTTGTAC CTGCCCACCC ATGGAAGTTT ATGAGTCTCC ATTTTCTGAA CAAAAGAGGG 3121 CGTCTTCTTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 3181 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 3241 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAGCTGT TTTCGAAGCG AGCATGCAAC 3301 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt