Transcript: Human NM_000170.2

Homo sapiens glycine decarboxylase (GLDC), mRNA.

Source:
NCBI, updated 2019-08-13
Taxon:
Homo sapiens (human)
Gene:
GLDC (2731)
Length:
3820
CDS:
194..3256

Additional Resources:

NCBI RefSeq record:
NM_000170.2
NBCI Gene record:
GLDC (2731)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_000170.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000036599 CGAGCCTACTTAAACCAGAAA pLKO.1 2081 CDS 100% 4.950 6.930 N GLDC n/a
2 TRCN0000307896 CGAGCCTACTTAAACCAGAAA pLKO_005 2081 CDS 100% 4.950 6.930 N GLDC n/a
3 TRCN0000120387 CCAGGGCAAATGTTTACATTT pLKO.1 3644 3UTR 100% 1.320 1.056 N Gldc n/a
4 TRCN0000303305 ATATTGGCATGGGCTATTATA pLKO_005 591 CDS 100% 15.000 10.500 N GLDC n/a
5 TRCN0000036601 CCACGGAAACTGCGATATTAA pLKO.1 2679 CDS 100% 15.000 10.500 N GLDC n/a
6 TRCN0000036603 GAAGTTTATGAGTCTCCATTT pLKO.1 3209 CDS 100% 10.800 7.560 N GLDC n/a
7 TRCN0000291954 GAAGTTTATGAGTCTCCATTT pLKO_005 3209 CDS 100% 10.800 7.560 N GLDC n/a
8 TRCN0000303371 TGTAATCTCTGTCAAGGTAAA pLKO_005 3378 3UTR 100% 10.800 7.560 N GLDC n/a
9 TRCN0000036600 CCTGCCAACATCCGTTTGAAA pLKO.1 479 CDS 100% 5.625 3.938 N GLDC n/a
10 TRCN0000036602 GCCACTGGGAAAGAAGTGTAT pLKO.1 1256 CDS 100% 4.950 3.465 N GLDC n/a
11 TRCN0000291953 GCCACTGGGAAAGAAGTGTAT pLKO_005 1256 CDS 100% 4.950 3.465 N GLDC n/a
12 TRCN0000120390 GCTGGAGAGTTTACTCAACTA pLKO.1 709 CDS 100% 4.950 2.970 N Gldc n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_000170.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06282 pDONR223 100% 99.9% 100% None 249G>A n/a
2 ccsbBroad304_06282 pLX_304 0% 99.9% 100% V5 249G>A n/a
3 TRCN0000475831 AGCTGTTTTCGAAGCGAGCATGCA pLX_317 11.8% 99.9% 100% V5 249G>A n/a
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