Construct: ORF TRCN0000475837
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008102.1_s317c1
- Derived from:
- ccsbBroadEn_02435
- DNA Barcode:
- AAATCACTCTTTCATCCATTGCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- N4BP2L2 (10443)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475837
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10443 | N4BP2L2 | NEDD4 binding protein 2 like 2 | NM_014887.3 | 100% | 100% | |
| 2 | human | 10443 | N4BP2L2 | NEDD4 binding protein 2 like 2 | XM_017020347.2 | 100% | 100% | |
| 3 | human | 10443 | N4BP2L2 | NEDD4 binding protein 2 like 2 | XM_017020348.2 | 100% | 100% | |
| 4 | human | 10443 | N4BP2L2 | NEDD4 binding protein 2 like 2 | XM_017020349.1 | 100% | 100% | |
| 5 | human | 10443 | N4BP2L2 | NEDD4 binding protein 2 like 2 | XM_011534874.3 | 97.5% | 97% | 1699_1699delAinsTGGGGAGGCTCTCT;1703T>C;1710_1710delTins27 |
| 6 | human | 10443 | N4BP2L2 | NEDD4 binding protein 2 like 2 | NM_001320836.2 | 48.7% | 47.8% | (many diffs) |
| 7 | human | 10443 | N4BP2L2 | NEDD4 binding protein 2 like 2 | XM_017020328.2 | 48.7% | 47.8% | (many diffs) |
| 8 | human | 10443 | N4BP2L2 | NEDD4 binding protein 2 like 2 | XM_017020329.2 | 48.7% | 47.8% | (many diffs) |
| 9 | human | 10443 | N4BP2L2 | NEDD4 binding protein 2 like 2 | XM_017020330.2 | 48.7% | 47.8% | (many diffs) |
| 10 | human | 10443 | N4BP2L2 | NEDD4 binding protein 2 like 2 | XM_017020324.2 | 48.2% | 47.3% | (many diffs) |
| 11 | human | 10443 | N4BP2L2 | NEDD4 binding protein 2 like 2 | XM_017020325.2 | 48.2% | 47.3% | (many diffs) |
| 12 | human | 10443 | N4BP2L2 | NEDD4 binding protein 2 like 2 | XM_017020326.1 | 48.2% | 47.3% | (many diffs) |
| 13 | human | 10443 | N4BP2L2 | NEDD4 binding protein 2 like 2 | XM_017020327.1 | 48.2% | 47.3% | (many diffs) |
| 14 | human | 10443 | N4BP2L2 | NEDD4 binding protein 2 like 2 | XM_024449305.1 | 48.2% | 47.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1815
- ORF length:
- 1749
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggaatgtc ttatggtgaa attgaaggta aattcttggg acctagagaa gaagtaacga 121 gtgagccacg ctgtaaaaaa ttgaagtcaa ccacagagtc gtatgttttt cacaatcata 181 gtaatgctga ttttcacaga atccaagaga aaactggaaa tgattgggtc cctgtgacca 241 tcattgatgt cagaggacat agttatttgc aggagaacaa aatcaaaact acagatttgc 301 atagaccttt gcatgatgag atgcctggta atagaccaga tgttattgaa tccattgatt 361 cacaggtttt acaggaagca cgtcctccat tagtatccgc agacgatgag atatatagca 421 caagtaaagc atttatagga cccatttaca aaccccctga gaaaaagaaa cgtaatgaag 481 ggaggaatga ggcacatgtt ctaaatggta taaatgacag aggaggacaa aaagagaaac 541 agaaatttaa ctctgaaaaa tcagagattg acaatgaatt attccagttt tacaaagaaa 601 ttgaagagct tgaaaaggaa aaagatggtt ttgagaacag ttgtaaagaa tctgaacctt 661 ctcaggaaca atttgttcca ttttatgagg gtcataataa tggtctctta aaacctgatg 721 aagaaaagaa agatcttagt aataaagcta tgccatcaca ttgtgattat cagcagaact 781 tggggaatga gccagacaaa tatccctgta atggacaagt aatacctaca ttttgtgaca 841 cttcatttac ttctttcagg cctgaatggc agtcagtata tccttttata gtgccctatg 901 gtccccctct tcccagtttg aactatcatt taaacattca gagattcagt ggtccaccaa 961 atccaccatc aaatattttc caagcccaag atgactctca gatacaaaat ggatattatg 1021 taaataattg tcatgttaac tggaattgca tgacttttga tcagaacaat gaatatactg 1081 actgtagtga gaataggagt agtgttcatc cctctggaaa tggctgcagt atgcaagatc 1141 gatatgtgag taatggtttc tgtgaagtca gagaaagatg ctggaaagat cattgtatgg 1201 acaagcataa tggaacagac aggtttgtga accagcagtt tcaagaggaa aagttaaata 1261 aattgcagaa gttacttatt cttttaagag gtctgcctgg ttctgggaaa acaacattgt 1321 ctcgaattct gcttggtcag aatcgtgatg gcattgtgtt cagcactgat gactattttc 1381 accatcaaga tgggtacagg tataatgtta atcaacttgg tgatgcccat gactggaacc 1441 agaacagagc aaaacaagct atcgatcagg gaagatctcc agttataata gataacacta 1501 atatacaagc ttgggaaatg aagccatatg tggaagtggC CATAGGAAAA GGATACAGAG 1561 TAGAGTTTCA TGAACCTGAA ACTTGGTGGA AATTTGATCC TGAAGAATTA GAAAAGAGGA 1621 ATAAACATGG TGTGTCTCGA AAGAAGATTG CTCAGATGTT GGATCGTTAT GAATATCAAA 1681 TGTCCATTTC TATTGTAATG AATTCAGTGG AACCATCACA CAAAAGCACA CAAAGACCTC 1741 CTCCTCCACA GGGGAGACAG AGGTGGGGAG GCTCTCTTGG CTCACATAAT CGTGTCTGTG 1801 TCACAAATAA TCATTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1861 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1921 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AAATCACTCT TTCATCCATT 1981 GCCAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt