Construct: ORF TRCN0000475864
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002445.1_s317c1
- Derived from:
- ccsbBroadEn_05772
- DNA Barcode:
- GGGGCCGCAGTTCGCTGGTCGATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADCY2 (108)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475864
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 108 | ADCY2 | adenylate cyclase 2 | NM_020546.3 | 99.9% | 99.9% | 439G>T;1878C>T;2661C>T |
| 2 | human | 108 | ADCY2 | adenylate cyclase 2 | XM_011513942.2 | 95.6% | 95.6% | (many diffs) |
| 3 | human | 108 | ADCY2 | adenylate cyclase 2 | XR_427657.2 | 82.2% | (many diffs) | |
| 4 | human | 108 | ADCY2 | adenylate cyclase 2 | XR_001741974.2 | 80.5% | (many diffs) | |
| 5 | human | 108 | ADCY2 | adenylate cyclase 2 | XR_001741973.1 | 70.2% | (many diffs) | |
| 6 | mouse | 210044 | Adcy2 | adenylate cyclase 2 | NM_153534.2 | 88.1% | 95.1% | (many diffs) |
| 7 | mouse | 210044 | Adcy2 | adenylate cyclase 2 | XR_001780768.2 | 67.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3342
- ORF length:
- 3273
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtggcaggag gcgatgcggc gccgccgcta cctgcgggac cgctccgagg 121 aggcggcggg cggcggagac gggctgccgc ggtcccggga ctggctctac gagtcctact 181 actgcatgag ccagcagcac ccgctcatcg tcttcctgct gctcatcgtc atgggctcct 241 gcctcgccct gctcgccgtc ttcttcgcgc tcgggctgga agttgaagac catgtggcgt 301 ttctaataac agttccaact gccctggcga ttttctttgc gatatttatc ctggtctgca 361 tcgagtctgt gtttaagaag ctgctgcgcc tcttctcgtt ggtgatatgg atatgccttg 421 ttgccatggg atacctgttc atgtgttttg gaggcaccgt ctctccctgg gaccaggtat 481 cgttcttcct cttcatcatc ttcgtgttgt acaccatgct gcccttcaac atgcgagacg 541 ccatcattgc cagcgtcctc acctcctcct cccacaccat cgtgcttagc gtctgcctgt 601 ctgcaacacc gggaggcaag gagcacctgg tctggcagat cctggccaat gtgatcattt 661 tcatctgtgg gaacctggcg ggagcctacc ataagcacct catggaactc gctcttcagc 721 aaacatatca ggacacctgt aattgcatca agtcgcggat caagttggaa tttgaaaaac 781 gtcaacagga gcggcttctg ctctccctgc tgccggccca catcgccatg gagatgaaag 841 cggagatcat ccagaggctg cagggcccca aggcgggcca gatggagaac acaaataact 901 tccacaacct gtatgtgaag cggcatacaa acgtgagcat cttatacgct gacatcgttg 961 gctttacccg gctggcaagt gactgctccc cgggagaact agtccacatg ctgaatgagc 1021 tctttggaaa gtttgatcaa attgcaaagg agaatgaatg catgagaatt aaaattttag 1081 gagactgcta ctactgtgta tctggactcc ctatatctct ccctaaccat gccaagaact 1141 gtgtgaaaat ggggctggac atgtgtgaag ccataaagaa agtgagggat gctactggag 1201 ttgatatcaa catgcgcgtg ggcgtgcatt ctgggaatgt cctgtgtggc gtgattggtc 1261 tgcagaagtg gcaatatgat gtgtggtcac atgatgtgac cttggccaac cacatggaag 1321 ctggaggggt ccctggacgt gttcacattt cttctgtcac cctggagcac ttgaatggcg 1381 cttataaagt ggaggaggga gatggtgaca ttagggaccc atatttaaaa cagcacctgg 1441 tgaaaaccta ctttgtgatc aaccccaagg gagaacgacg gagcccccag catctcttca 1501 gacctcgcca cacccttgat ggagccaaaa tgagggcctc ggtccgcatg acccggtact 1561 tggagtcctg gggggcagcc aagccctttg cacacctaca tcacagggac agcatgacca 1621 cagagaacgg caagatcagc accacggatg tacccatggg tcagcataat tttcaaaatc 1681 gcaccttaag aaccaagtca caaaagaaga gatttgaaga agaattgaat gaaaggatga 1741 ttcaagcaat tgatgggatt aatgcacaga agcaatggct caagtctgaa gacattcaga 1801 gaatctcact gcttttctat aacaaagtac tagaaaaaga gtaccgggcc acggcactgc 1861 cagcgttcaa gtattatgtg acttgtgcct gtctcatatt cttctgcatc ttcattgtgc 1921 agattctcgt gctgccaaaa acgtctgtcc tgggcatctc ctttggggct gcgtttctct 1981 tgctggcctt catcctcttc gtctgctttg ctggacagct tctgcaatgc agcaaaaaag 2041 cctctcccct gctcatgtgg cttttgaagt cctcgggcat cattgccaac cgcccctggc 2101 cacggatctc tctcacgatc atcaccacag ccatcatatt aatgatggcc gtgttcaaca 2161 tgtttttcct gagtgactca gaggaaacaa tccctccaac tgccaacaca acaaacacaa 2221 gcttttcagc ctcaaataat caggtggcga ttctgcgtgc gcagaattta tttttcctcc 2281 cgtactttat ctacagctgc attctgggac tgatatcctg ttccgtgttc ctgcgggtaa 2341 actatgagct gaagatgttg atcatgatgg tggccttggt gggctacaac accatcctac 2401 tccacaccca cgcccacgtc ctgggcgact acagccaggt cttatttgag agaccaggca 2461 tttggaaaga cctgaagacc atgggctctg tgtctctctc tatattcttc atcacactgc 2521 ttgttctggg tagacagaat gaatattact gtaggttaga cttcttatgg aagaacaaat 2581 tcaaaaaaga gcgggaggag atagagacca tggagaacct gaaccgcgtg ctgctggaga 2641 acgtgcttcc cgcgcacgtg gctgagcact tcctggccag gagcctgaag aatgaggagc 2701 tataccacca gtcctatgac tgcgtctgtg tcatgtttgc ctccattccg gatttcaaag 2761 aattttatac agaatccgac gtgaacaagg agggcttgga atgccttcgg ctcctgaacg 2821 agatcatcgc tgactttgat gatcttcttt ccaagccaaa attcagtgga gttgaaaaga 2881 ttaagaccat tggcagcaca tacatggcag caacaggtct gagcgctgtg cccagccagg 2941 agcactccca ggagcccgag cggcagtaca tgcacattGG CACCATGGTG GAGTTTGCTT 3001 TTGCCCTGGT AGGGAAGCTG GATGCCATCA ACAAGCACTC CTTCAACGAC TTCAAATTGC 3061 GAGTGGGTAT TAACCATGGA CCTGTGATAG CTGGTGTGAT TGGAGCTCAG AAGCCACAAT 3121 ATGATATCTG GGGCAACACT GTCAATGTGG CCAGTAGGAT GGACAGCACC GGAGTCCTGG 3181 ACAAAATACA GGTTACCGAG GAGACGAGCC TCGTCCTGCA GACCCTCGGA TACACGTGCA 3241 CCTGTCGAGG AATAATCAAC GTGAAAGGAA AGGGGGACCT GAAGACGTAC TTTGTAAACA 3301 CAGAAATGTC AAGGTCCCTT TCCCAGAGCA ACGTGGCATC CTTGCCAACT TTCTTGTACA 3361 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 3421 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 3481 AGGACGAGGG GCCGCAGTTC GCTGGTCGAT CACGCGTTAA GTCgacaatc aacctctgga 3541 ttacaaaatt tgtgaaagat t