Construct: ORF TRCN0000475878
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013669.1_s317c1
- Derived from:
- ccsbBroadEn_06104
- DNA Barcode:
- ATGTTTCTAACCAGAGTCAGCAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SARDH (1757)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475878
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1757 | SARDH | sarcosine dehydrogenase | NM_001134707.2 | 99.9% | 99.8% | 1222G>T |
| 2 | human | 1757 | SARDH | sarcosine dehydrogenase | NM_007101.4 | 99.9% | 99.8% | 1222G>T |
| 3 | human | 1757 | SARDH | sarcosine dehydrogenase | XM_017014367.1 | 95.6% | 95.4% | 1222G>T;2632_2633insT;2634_2635ins119 |
| 4 | human | 1757 | SARDH | sarcosine dehydrogenase | XM_017014368.1 | 90.6% | 90.5% | (many diffs) |
| 5 | human | 1757 | SARDH | sarcosine dehydrogenase | XR_001746217.1 | 83.1% | (many diffs) | |
| 6 | human | 1757 | SARDH | sarcosine dehydrogenase | XR_001746218.1 | 82.6% | (many diffs) | |
| 7 | human | 1757 | SARDH | sarcosine dehydrogenase | XR_001746216.1 | 76.4% | 1_296del;1518G>T;3051_3601del | |
| 8 | human | 1757 | SARDH | sarcosine dehydrogenase | XR_929726.2 | 76.1% | 1_167del;1389G>T;2922_3613del | |
| 9 | human | 1757 | SARDH | sarcosine dehydrogenase | XR_001746213.1 | 73.5% | 1_296del;1518G>T;3051_3742del | |
| 10 | human | 1757 | SARDH | sarcosine dehydrogenase | XR_001746215.1 | 73.5% | 1_298del;1520G>T;3053_3744del | |
| 11 | human | 1757 | SARDH | sarcosine dehydrogenase | XR_002956762.1 | 56.9% | (many diffs) | |
| 12 | human | 1757 | SARDH | sarcosine dehydrogenase | XR_001746214.1 | 55.8% | 1_1479del;2701G>T;4234_4925del | |
| 13 | mouse | 192166 | Sardh | sarcosine dehydrogenase | NM_138665.2 | 84.9% | 88.4% | (many diffs) |
| 14 | mouse | 192166 | Sardh | sarcosine dehydrogenase | XM_006497788.3 | 84.9% | 88.4% | (many diffs) |
| 15 | mouse | 192166 | Sardh | sarcosine dehydrogenase | XM_006497789.3 | 84.9% | 88.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2823
- ORF length:
- 2754
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcctcactg agccgagccc tacgtgtggc tgctgcccac cctcgccaga 121 gccctacccg gggcatgggg ccatgcaacc tgtccagcgc agctggcccc acagccgaga 181 agagtgtgcc atatcagcgg accctgaagg agggacaggg cacctcggtg gtggcccaag 241 gcccaagccg gcccctgccc agcacggcca acgtggtggt cattggtgga ggcagcttgg 301 gctgccagac cctgtaccac ctggccaagc tgggcatgag tggggcggtg ctgctggagc 361 gggagcggct gacctccggg accacctggc acacggcagg cctgctgtgg cagctgcggc 421 ccagtgacgt ggaggtggag cttctggccc acactcggcg ggtggtgagc cgggagctgg 481 aggaggagac gggactacac acgggctgga tccagaatgg gggcctcttc atcgcgtcca 541 accggcagcg cctggacgag tacaagaggc tcatgtcgct gggcaaggcg tatggtgtgg 601 aatcccatgt gctgagcccg gcagagacca agactctgta cccgctgatg aatgtggacg 661 acctctacgg gaccctgtat gtgccgcacg acggtaccat ggaccccgct ggcacctgta 721 ccaccctcgc cagggcagct tctgcccgag gagcacaggt cattgagaac tgcccagtga 781 ccggcattcg tgtgtggacg gatgattttg gggtgcggcg ggtcgcgggt gtggagactc 841 agcatggttc catccagaca ccctgcgtgg tcaactgtgc aggagtgtgg gcaagtgctg 901 tgggccggat ggctggagtc aaggtcccgc tggtggccat gcaccatgcc tatgtcgtca 961 ccgagcgcat cgaggggatt cagaacatgc ccaatgtccg tgatcatgat gcctctgtct 1021 acctccgcct ccaaggggat gccttgtctg tgggtggcta tgaggccaac cccatctttt 1081 gggaggaggt gtcagacaag tttgccttcg gcctctttga cctggactgg gaggtgttca 1141 cccagcacat tgaaggcgcc atcaacaggg tccccgtgct ggagaagaca ggaatcaagt 1201 ccacggtctg cggccctgaa tccttcacgc ccgaccacaa gcccctgatg ggggaggcac 1261 ctgagctccg agggttcttc ctgggctgtt gcttcaacag cgcaggaatg atgctgggtg 1321 gtggctgtgg gcaggagctg gcccactgga tcatccatgg gcgcccggag aaggacatgc 1381 atggctatga catcaggcgc ttccatcact cgctcacgga ccacccccgc tggatccgag 1441 agcgaagcca tgagtcctac gccaagaact actccgtcgt cttcccccac gatgagccgc 1501 tggccgggcg caacatgagg agagacccgc tgcacgagga actccttgga caaggctgcg 1561 tgttccagga gcggcatggc tgggagcgac cgggatggtt tcatccccga ggcccagctc 1621 cggtcctcga gtacgactac tacggggctt acgggagccg cgcgcacgag gactacgcct 1681 accgcaggct gctggcagac gagtacacct tcgccttccc gccccaccac gacacgatca 1741 agaaggagtg cctggcctgc agaggggccg ccgctgtgtt tgacatgtcc tacttcggga 1801 agttctacct ggtggggctg gatgcaagga aggctgccga ctggctcttc tccgcagatg 1861 tcagccgacc cccaggctcc accgtgtaca cgtgcatgct caaccaccgt gggggcaccg 1921 agagtgacct gactgtcagc cgcctggcac ccagccacca ggcctccccg ctggcccccg 1981 cctttgaagg ggacggttac tacctggcca tgggcggggc cgtggcccag cacaactggt 2041 cccacatcac caccgtgctg caggaccaga agtcccagtg ccagctcatc gacagctccg 2101 aggacctggg tatgatcagt atccagggcc cagccagccg agccattttg caggaggtgc 2161 tggacgcaga cctgagcaac gaggccttcc cgttctccac ccacaagcta ctgagagccg 2221 cagggcacct ggtccgagcc atgcggctgt cctttgtggg ggagctgggc tgggagctgc 2281 acattccaaa ggcgtcctgc gtgcctgtgt accgggctgt gatggccgcg ggtgccaagc 2341 acggcctcat caacgcaggg taccgcgcca tcgactccct gagcattgag aaaggctacc 2401 ggcactggca cgcggacctg cggccagacg acagccccct ggaggcaggc ctggccttca 2461 cctgcaagct caagtcgccg gTGCCCTTCC TGGGGAGGGA GGCCCTGGAG CAGCAGCGGG 2521 CCGCAGGCCT CCGCCGGCGC CTGGTGTGCT TCACCATGGA GGACAAAGTA CCCATGTTTG 2581 GCCTGGAGGC CATCTGGAGG AACGGCCAAG TGGTGGGCCA TGTCCGGAGG GCTGACTTTG 2641 GGTTCGCCAT CGACAAGACC ATCGCCTACG GTTACATCCA TGACCCCAGC GGTGGGCCGG 2701 TCTCGCTGGA CTTTGTGAAG AGCGGGGACT ATGCCCTGGA GAGAATGGGG GTGACCTATG 2761 GTGCCCAGGC TCACCTGAAG TCGCCCTTCG ACCCCAACAA CAAGAGGGTG AAGGGAATCT 2821 ACTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 2881 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2941 GGCTTTATAT ATCTTGTGGA AAGGACGAAT GTTTCTAACC AGAGTCAGCA GCACGCGTTA 3001 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt