Construct: ORF TRCN0000475879
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017115.1_s317c1
- Derived from:
- ccsbBroadEn_14494
- DNA Barcode:
- CAAGAACGGTTTTTTCTGCCATGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GTF2IRD2B (389524)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475879
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 389524 | GTF2IRD2B | GTF2I repeat domain contain... | NM_001003795.3 | 99.9% | 99.5% | 2562C>T;2837_2838insC |
| 2 | human | 84163 | GTF2IRD2 | GTF2I repeat domain contain... | NM_173537.4 | 99.5% | 98.8% | (many diffs) |
| 3 | human | 389524 | GTF2IRD2B | GTF2I repeat domain contain... | NM_001368302.1 | 85.3% | 85% | 1_486del;3048C>T;3323_3324insC |
| 4 | human | 84163 | GTF2IRD2 | GTF2I repeat domain contain... | NM_001368300.1 | 85% | 84.4% | (many diffs) |
| 5 | human | 84163 | GTF2IRD2 | GTF2I repeat domain contain... | NM_001281447.1 | 10.4% | 8.8% | (many diffs) |
| 6 | human | 389524 | GTF2IRD2B | GTF2I repeat domain contain... | NM_001368301.1 | 10.4% | 8.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2919
- ORF length:
- 2850
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcccaggta gcagtgtcca ccctgcctgt tgaagaagag tcctcctcag 121 agaccaggat ggtggtgaca ttcctcgtgt ctgccctcga atccatgtgt aaagaactgg 181 ccaagtccaa ggcagaagtg gcctgcatcg cagtgtacga aacagacgtg tttgtcgtcg 241 gaaccgagag aggatgcgct tttgttaatg ccaggacgga ttttcagaaa gattttgcaa 301 aatactgcgt tgcagaggga ctgtgtgagg tgaaacctcc ctgccctgtg aacgggatgc 361 aggtccactc gggcgaaacg gaaatactca ggaaggcagt ggaggactat ttctgctttt 421 gttatggtaa agccttaggg acaacagtga tggtgcctgt tccctatgag aagatgctgc 481 gagaccagtc ggctgtggta gtgcaggggc ttccggaagg cgttgccttt caacaccctg 541 agaattacga ccttgcaacc ctgaaatgga ttttggagaa caaagcaggg atttcattca 601 tcataaatag acccttccta ggaccagaga gtcagctggg tggccctggg atggtaacag 661 atgcggagag atccatagta tcaccaagtg aaagctgcgg ccccatcaat gtgaaaactg 721 aacccatgga agattctggc atttcactga aagcagaagc tgtctcagtc aagaaagaat 781 cagaagatcc taattactat caatataata tgcaaggaag ccacccttct tccacaagca 841 atgaagtaat agaaatggaa ttaccaatgg aagattccac tccgctggtc ccttcagaag 901 aaccaaatga ggaccctgaa gccgaggtga aaatcgaagg aaacacaaat tcatccagtg 961 ttacaaattc tgcagcaggt gttgaagatc ttaacatcgt tcaagtgact gttccagata 1021 atgagaagga aagattatca agcattgaaa agattaaaca gctaagagaa caagttaatg 1081 acctctttag ccgaaaattt ggtgaagcaa ttggcgtgga tttccctgtg aaagttccct 1141 acaggaagat cacattcaac cctggctgtg tggtgattga tggcatgccc ccgggggtgg 1201 tattcaaggc ccccggctat ctggaaatca gttccatgag gaggatcttg gaggcagctg 1261 agtttatcaa attcacagtc atcaggccgc ttccagggct tgagctcagt aatgtgggaa 1321 aacgcaagat agaccaggag ggccgtgtgt ttcaagaaaa gtgggagaga gcgtatttct 1381 tcgtggaagt acagaatatt ccaacatgtc tcatatgcaa acaaagcatg tctgtgtcca 1441 aagaatataa cctaagacgc cactatcaaa ccaatcacag caagcattat gaccagtata 1501 cggaaagaat gcgtgacgag aagcttcacg agctgaaaaa agggctcagg aagtatctct 1561 taggctcgtc agacaccgag tgtcccgagc aaaaacaagt gtttgcaaac ccaagtccaa 1621 cccagaaatc ccccgtgcag cctgtagagg acctagctgg gaacttatgg gagaagttac 1681 gtgaaaaaat caggtctttt gtggcatatt ctatcgcaat cgatgagatc acggatataa 1741 ataataccac ccagttggcc atattcatcc gtggtgtcga tgagaatttc gatgtgtccg 1801 aagaacttct ggacacggtg cccatgacgg gtacaaaatc tggcaacgag atctttttgc 1861 gtgttgagaa gagcctgaaa aagttctgta tcaactggtc gagattagta agcgtggcct 1921 ccactggcac cccagcgatg gtggatgcca ataacgggct tgtcacaaaa ctgaagtcca 1981 gggtggcgac gttctgcaag ggtgcggaac tgaagtccat ctgttgtata attcatccgg 2041 aatcactctg tgctcagaag ttgaagatgg accacgtcat ggacgtggta gtgaagtccg 2101 tgaactggat atgctcccgg ggactgaacc acagcgagtt cacaaccttg ctctatgagc 2161 tggacagcca gtatggtagc ctcctgtact acacggagat taagtggctc agtcgcgggc 2221 tcgtgctaaa gagatttttc gaatccttgg aagaaatcga ctccttcatg tcatccagag 2281 ggaaacccct gcctcaactg agctccatag attggatccg agacctggcc ttcttggttg 2341 acatgacgat gcatctgaac gctttgaaca tctctctcca aggacactcc caaatcgtca 2401 cgcagatgta tgacctgatc cgggcgttcc tagcaaaact gtgcctctgg gagactcatt 2461 tgacgaggaa taatctggcc cactttccca ccctgaaatt ggtttccaga aatgaaagcg 2521 atggcctgaa ctacattccc aaaatcgcgg aactcaagac cgaattccag aaaaGGCTGT 2581 CTGATTTCAA ACTCTACGAA AGCGAACTGA CTCTGTTCAG CTCCCCGTTT TCCACGAAGA 2641 TCGACAGTGT GCACGAGGAG CTCCAGATGG AGGTTATCGA CCTGCAATGC AACACGGTCC 2701 TGAAGACGAA ATACGACAAG GTGGGAATAC CAGAATTCTA CAAGTACCTC TGGGGTAGCT 2761 ACCCGAAATA CAAGCACCAT TGCGCAAAGA TTCTTTCCAT GTTCGGGAGC ACCTACATCT 2821 GCGAACAGCT GTTCTCCATT ATGAAACTGA GCAAAACAAA ATACTGCTCC CAGTTAAAGG 2881 ATTCCCAGTG GGATTCTGTA CTCCACCATC GCAACGTTGC CAACTTTCTT GTACAAAGTG 2941 GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA 3001 ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC 3061 GACAAGAACG GTTTTTTCTG CCATGCACGC GTTAAGTCga caatcaacct ctggattaca 3121 aaatttgtga aagatt