Construct: ORF TRCN0000475911
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001874.1_s317c1
- Derived from:
- ccsbBroadEn_00038
- DNA Barcode:
- AGGACCATAGCAGTTATTCAGTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AGL (178)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475911
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 178 | AGL | amylo-alpha-1, 6-glucosidas... | NM_000028.2 | 100% | 100% | |
| 2 | human | 178 | AGL | amylo-alpha-1, 6-glucosidas... | NM_000642.3 | 100% | 100% | |
| 3 | human | 178 | AGL | amylo-alpha-1, 6-glucosidas... | NM_000643.2 | 100% | 100% | |
| 4 | human | 178 | AGL | amylo-alpha-1, 6-glucosidas... | NM_000644.2 | 100% | 100% | |
| 5 | human | 178 | AGL | amylo-alpha-1, 6-glucosidas... | XM_005270557.2 | 100% | 100% | |
| 6 | human | 178 | AGL | amylo-alpha-1, 6-glucosidas... | NM_000646.2 | 98.2% | 98.4% | (many diffs) |
| 7 | human | 178 | AGL | amylo-alpha-1, 6-glucosidas... | XM_017000501.2 | 62.1% | 62.1% | 0_1ins1740 |
| 8 | mouse | 77559 | Agl | amylo-1,6-glucosidase, 4-al... | NM_001081326.1 | 86.1% | 91.9% | (many diffs) |
| 9 | mouse | 77559 | Agl | amylo-1,6-glucosidase, 4-al... | XM_006502287.1 | 86.1% | 91.9% | (many diffs) |
| 10 | mouse | 77559 | Agl | amylo-1,6-glucosidase, 4-al... | XM_011240280.2 | 56% | 60.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 4662
- ORF length:
- 4596
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg acacagtaaa cagattcgaa ttttacttct gaacgaaatg gagaaactgg 121 aaaagaccct cttcagactt gaacaagggt atgagctaca gttccgatta ggcccaactt 181 tacagggaaa agcagttacc gtgtatacaa attacccatt tcctggagaa acatttaata 241 gagaaaaatt ccgttctctg gattgggaaa atccaacaga aagagaagat gattctgata 301 aatactgtaa acttaatctg caacaatctg gttcatttca gtattatttc cttcaaggaa 361 atgagaaaag tggtggaggt tacatagttg tggaccccat tttacgtgtt ggtgctgata 421 atcatgtgct acccttggac tgtgttactc ttcagacatt tttagctaag tgtttgggac 481 cttttgatga atgggaaagc agacttaggg ttgcaaaaga atcaggctac aacatgattc 541 attttacccc attgcagact cttggactat ctaggtcatg ctactccctt gccaatcagt 601 tagaattaaa tcctgacttt tcaagaccta atagaaagta tacctggaat gatgttggac 661 agctagtgga aaaattaaaa aaggaatgga atgttatttg tattactgat gttgtctaca 721 atcatactgc tgctaatagt aaatggatcc aggaacatcc agaatgtgcc tataatcttg 781 tgaattctcc acacttaaaa cctgcctggg tcttagacag agcactttgg cgtttctcct 841 gtgatgttgc agaagggaaa tacaaagaaa agggaatacc tgctttgatt gaaaatgatc 901 accatatgaa ttccatccga aaaataattt gggaggatat ttttccaaag cttaaactct 961 gggaattttt ccaagtagat gtcaacaaag cggttgagca atttagaaga cttcttacac 1021 aagaaaatag gcgagtaacc aagtctgatc caaaccaaca ccttacgatt attcaagatc 1081 ctgaatacag acggtttggc tgtactgtag atatgaacat tgcactaacg actttcatac 1141 cacatgacaa ggggccagca gcaattgaag aatgctgtaa ttggtttcat aaaagaatgg 1201 aggaattaaa ttcagagaag catcgactca ttaactatca tcaggaacag gcagttaatt 1261 gccttttggg aaatgtgttt tatgaacgac tggctggcca tggtccaaaa ctaggacctg 1321 tcactagaaa gcatccttta gttaccaggt attttacttt cccatttgaa gagatagact 1381 tctccatgga agaatctatg attcatctgc caaataaagc ttgttttctg atggcacaca 1441 atggatgggt aatgggagat gatcctcttc gaaactttgc tgaaccgggt tcagaagttt 1501 acctaaggag agaacttatt tgctggggag acagtgttaa attacgctat gggaataaac 1561 cagaggactg tccttatctc tgggcacaca tgaaaaaata cactgaaata actgcaactt 1621 atttccaggg agtacgtctt gataactgcc actcaacacc tcttcacgta gctgagtaca 1681 tgttggatgc tgctaggaat ttgcaaccca atttatatgt agtagctgaa ctgttcacag 1741 gaagtgaaga tctggacaat gtctttgtta ctagactggg cattagttcc ttaataagag 1801 aggcaatgag tgcatataat agtcatgaag agggcagatt agtttaccga tatggaggag 1861 aacctgttgg atcctttgtt cagccctgtt tgaggccttt aatgccagct attgcacatg 1921 ccctgtttat ggatattacg catgataatg agtgtcctat tgtgcataga tcagcgtatg 1981 atgctcttcc aagtactaca attgtttcta tggcatgttg tgctagtgga agtacaagag 2041 gctatgatga attagtgcct catcagattt cagtggtttc tgaagaacgg ttttacacta 2101 agtggaatcc tgaagcattg ccttcaaaca caggtgaagt taatttccaa agcggcatta 2161 ttgcagccag gtgtgctatc agtaaacttc atcaggagct tggagccaag ggttttattc 2221 aggtgtatgt ggatcaagtt gatgaagaca tagtggcagt aacaagacac tcacctagca 2281 tccatcagtc tgttgtggct gtatctagaa ctgctttcag gaatcccaag acttcatttt 2341 acagcaagga agtgcctcaa atgtgcatcc ctggcaaaat tgaagaagta gttcttgaag 2401 ctagaactat tgagagaaac acgaaacctt ataggaagga tgagaattca atcaatggaa 2461 caccagatat cacagtagaa attagagaac atattcagct taatgaaagt aaaattgtta 2521 aacaagctgg agttgccaca aaagggccca atgaatatat tcaagaaata gaatttgaaa 2581 acttgtctcc aggaagtgtt attatattca gagttagtct tgatccacat gcacaagtcg 2641 ctgttggaat tcttcgaaat catctgacac aattcagtcc tcactttaaa tctggcagcc 2701 tagctgttga caatgcagat cctatattaa aaattccttt tgcttctctt gcctccagat 2761 taactttggc tgagctaaat cagatccttt accgatgtga atcagaagaa aaggaagatg 2821 gtggagggtg ctatgacata ccaaactggt cagcccttaa atatgcaggt cttcaaggtt 2881 taatgtctgt attggcagaa ataagaccaa agaatgactt ggggcatcct ttttgtaata 2941 atttgagatc tggagattgg atgattgact atgtcagtaa ccggcttatt tcacgatcag 3001 gaactattgc tgaagttggt aaatggttgc aggctatgtt cttctacctg aagcagatcc 3061 cacgttacct tatcccatgt tactttgatg ctatattaat tggtgcatat accactcttc 3121 tggatacagc atggaagcag atgtcaagct ttgttcagaa tggttcaacc tttgtgaaac 3181 acctttcatt gggttcagtt caactgtgtg gagtaggaaa attcccttcc ctgccaattc 3241 tttcacctgc cctaatggat gtaccttata ggttaaatga gatcacaaaa gaaaaggagc 3301 aatgttgtgt ttctctagct gcaggcttac ctcatttttc ttctggtatt ttccgctgct 3361 ggggaaggga tacttttatt gcacttagag gtatactgct gattactgga cgctatgtag 3421 aagccaggaa tattatttta gcatttgcgg gtaccctgag gcatggtctc attcctaatc 3481 tactgggtga aggaatttat gccagataca attgtcggga tgctgtgtgg tggtggctgc 3541 agtgtatcca ggattactgt aaaatggttc caaatggtct agacattctc aagtgcccag 3601 tttccagaat gtatcctaca gatgattctg ctcctttgcc tgctggcaca ctggatcagc 3661 cattgtttga agtcatacag gaagcaatgc aaaaacacat gcagggcata cagttccgag 3721 aaaggaatgc tggtccccag atagatcgaa acatgaagga cgaaggtttt aatataactg 3781 caggagttga tgaagaaaca ggatttgttt atggaggaaa tcgtttcaat tgtggcacat 3841 ggatggataa aatgggagaa agtgacagag ctagaaacag aggaatccca gccacaccaa 3901 gagatgggtc tgctgtggaa attgtgggcc tgagtaaatc tgctgttcgc tggttgctgg 3961 aattatccaa aaaaaatatt ttcccttatc atgaagtcac agtaaaaaga catggaaagg 4021 ctataaaggt ctcatatgat gagtggaaca gaaaaataca agacaacttt gaaaagctat 4081 ttcatgtttc cgaagaccct tcagatttaa atgaaaagca tccaaatctg gttcacaaac 4141 gtggcatata caaagatagt tatggagctt caagtccttg gtgtgactat cagctcaggc 4201 ctaattttac catagcaatg gttgtggccc ctgagctctt tactacagaa aaagcatgga 4261 aagctttgga gattgcagaa aaaaaattgc ttggtcccct tggcatgaaa actttagatc 4321 cagatgatat ggtttactgt ggaatttatg acaatgcatt agacaatgac aactacaaTC 4381 TTGCTAAAGG TTTCAATTAT CACCAAGGAC CTGAGTGGCT GTGGCCTATT GGGTATTTTC 4441 TTCGTGCAAA ATTATATTTT TCCAGATTGA TGGGCCCGGA GACTACTGCA AAGACTATAG 4501 TTTTGGTTAA AAATGTTCTT TCCCGACATT ATGTTCATCT TGAGAGATCC CCTTGGAAAG 4561 GACTTCCAGA ACTGACCAAT GAGAATGCCC AGTACTGTCC TTTCAGCTGT GAAACACAAG 4621 CCTGGTCAAT TGCTACTATT CTTGAGACAC TTTATGATTT ATACCCAACT TTCTTGTACA 4681 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 4741 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 4801 AGGACGAAGG ACCATAGCAG TTATTCAGTT TACGCGTTAA GTCgacaatc aacctctgga 4861 ttacaaaatt tgtgaaagat t