Construct: ORF TRCN0000475924
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011428.1_s317c1
- Derived from:
- ccsbBroadEn_09892
- DNA Barcode:
- GCTTCAGACGATGTCCCAGACCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HEPACAM2 (253012)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475924
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 253012 | HEPACAM2 | HEPACAM family member 2 | NM_198151.4 | 100% | 100% | |
| 2 | human | 253012 | HEPACAM2 | HEPACAM family member 2 | NM_001039372.4 | 96.5% | 93.1% | (many diffs) |
| 3 | human | 253012 | HEPACAM2 | HEPACAM family member 2 | NM_001288810.3 | 95.3% | 79.9% | 1100_1101ins25;1326_1365del |
| 4 | human | 253012 | HEPACAM2 | HEPACAM family member 2 | NM_001288804.3 | 91.8% | 88.4% | (many diffs) |
| 5 | human | 253012 | HEPACAM2 | HEPACAM family member 2 | NM_001346642.1 | 86% | 86% | 0_1ins189 |
| 6 | human | 253012 | HEPACAM2 | HEPACAM family member 2 | XM_011516001.2 | 86% | 86% | 0_1ins189 |
| 7 | mouse | 101202 | Hepacam2 | HEPACAM family member 2 | XM_006504957.2 | 82.5% | 82.7% | (many diffs) |
| 8 | mouse | 101202 | Hepacam2 | HEPACAM family member 2 | NM_178899.5 | 82.5% | 82.5% | (many diffs) |
| 9 | mouse | 101202 | Hepacam2 | HEPACAM family member 2 | XM_006504958.3 | 82.4% | 82.2% | (many diffs) |
| 10 | mouse | 101202 | Hepacam2 | HEPACAM family member 2 | XM_006504960.2 | 73.8% | 74.6% | (many diffs) |
| 11 | mouse | 101202 | Hepacam2 | HEPACAM family member 2 | XM_017321287.1 | 73.8% | 74.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1419
- ORF length:
- 1350
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtggctcaag gtcttcacaa ctttcctttc ctttgcaaca ggtgcttgct 121 cggggctgaa ggtgacagtg ccatcacaca ctgtccatgg cgtcagaggt caggccctct 181 acctacccgt ccactatggc ttccacactc cagcatcaga catccagatc atatggctat 241 ttgagagacc ccacacaatg cccaaatact tactgggctc tgtgaataag tctgtggttc 301 ctgacttgga ataccaacac aagttcacca tgatgccacc caatgcatct ctgcttatca 361 acccactgca gttccctgat gaaggcaatt acatcgtgaa ggtcaacatt cagggaaatg 421 gaactctatc tgccagtcag aagatacaag tcacggttga tgatcctgtc acaaagccag 481 tggtgcagat tcatcctccc tctggggctg tggagtatgt ggggaacatg accctgacat 541 gccatgtgga agggggcact cggctagctt accaatggct aaaaaatggg agacctgtcc 601 acaccagctc cacctactcc ttttctcccc aaaacaatac ccttcatatt gctccagtaa 661 ccaaggaaga cattgggaat tacagctgcc tggtgaggaa ccctgtcagt gaaatggaaa 721 gtgatatcat tatgcccatc atatattatg gaccttatgg acttcaagtg aattctgata 781 aagggctaaa agtaggggaa gtgtttactg ttgaccttgg agaggccatc ctatttgatt 841 gttctgctga ttctcatccc cccaacacct actcctggat taggaggact gacaatacta 901 catatatcat taagcatggg cctcgcttag aagttgcatc tgagaaagta gcccagaaga 961 caatggacta tgtgtgctgt gcttacaaca acataaccgg caggcaagat gaaactcatt 1021 tcacagttat catcacttcc gtaggactgg agaagcttgc acagaaagga aaatcattgt 1081 cacctttagc aagtataact gGAATATCAC TATTTTTGAT TATATCCATG TGTCTTCTCT 1141 TCCTATGGAA AAAATATCAA CCCTACAAAG TTATAAAACA GAAACTAGAA GGCAGGCCAG 1201 AAACAGAATA CAGGAAAGCT CAAACATTTT CAGGCCATGA AGATGCTCTG GATGACTTCG 1261 GAATATATGA ATTTGTTGCT TTTCCAGATG TTTCTGGTGT TTCCAGGATC CCAAGCAGGT 1321 CTGTTCCAGC CTCTGATTGT GTATCGGGGC AAGATTTGCA CAGTACAGTG TATGAAGTTA 1381 TTCAGCACAT CCCTGCCCAG CAGCAAGACC ATCCAGAGTT GCCAACTTTC TTGTACAAAG 1441 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1501 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1561 ACGAGCTTCA GACGATGTCC CAGACCTAAC GCGTTAAGTC gacaatcaac ctctggatta 1621 caaaatttgt gaaagatt