Construct: ORF TRCN0000475973
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002264.1_s317c1
- Derived from:
- ccsbBroadEn_04780
- DNA Barcode:
- AGATTAGTCTCTCAATTGTTTGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPR139 (124274)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475973
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 124274 | GPR139 | G protein-coupled receptor 139 | NM_001002911.3 | 100% | 100% | |
| 2 | human | 124274 | GPR139 | G protein-coupled receptor 139 | NM_001318483.1 | 73.6% | 73.6% | 0_1ins279 |
| 3 | mouse | 209776 | Gpr139 | G protein-coupled receptor 139 | NM_001024138.1 | 88% | 94% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1128
- ORF length:
- 1059
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagcacacg cacgcccacc tcgcagccaa cagctcgctg tcttggtggt 121 cccccggctc ggcctgcggc ttgggtttcg tgcccgtggt ctactacagc ctcttgctgt 181 gcctcggttt accagcaaat atcttgacag tgatcatcct ctcccagctg gtggcaagaa 241 gacagaagtc ctcctacaac tatctcttgg cactcgctgc tgccgacatc ttggtcctct 301 ttttcatagt gtttgtggac ttcctgttgg aagatttcat cttgaacatg cagatgcctc 361 aggtccccga caagatcata gaagtgctgg aattctcatc catccacacc tccatatgga 421 ttactgtacc gttaaccatt gacaggtata tcgctgtctg ccacccgctc aagtaccaca 481 cggtctcata cccagcccgc acccggaaag tcattgtaag tgtttacatc acctgcttcc 541 tgaccagcat cccctattac tggtggccca acatctggac tgaagactac atcagcacct 601 ctgtgcatca cgtcctcatc tggatccact gcttcaccgt ctacctggtg ccctgctcca 661 tcttcttcat cttgaactca atcattgtgt acaagctcag gaggaagagc aattttcgtc 721 tccgtggcta ctccacgggg aagaccaccg ccatcttgtt caccattacc tccatcttTG 781 CCACACTTTG GGCCCCCCGC ATCATCATGA TTCTTTACCA CCTCTATGGG GCGCCCATCC 841 AGAACCGCTG GCTGGTACAC ATCATGTCCG ACATTGCCAA CATGCTAGCC CTTCTGAACA 901 CAGCCATCAA CTTCTTCCTC TACTGCTTCA TCAGCAAGCG GTTCCGCACC ATGGCAGCCG 961 CCACGCTCAA GGCTTTCTTC AAGTGCCAGA AGCAACCTGT ACAGTTCTAC ACCAATCATA 1021 ACTTTTCCAT AACAAGTAGC CCCTGGATCT CGCCGGCAAA CTCACACTGC ATCAAGATGC 1081 TGGTGTACCA GTATGACAAA AATGGAAAAC CTATAAAAGT ATCCCCGTTG CCAACTTTCT 1141 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1201 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1261 GTGGAAAGGA CGAAGATTAG TCTCTCAATT GTTTGCCACG CGTTAAGTCg acaatcaacc 1321 tctggattac aaaatttgtg aaagatt