Construct: ORF TRCN0000475982

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006843.1_s317c1
Derived from:
ccsbBroadEn_12418
DNA Barcode:
TTCACTGTACTAGCTTGCTTTCTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EPS15L1 (58513)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475982
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 58513 EPS15L1 epidermal growth factor rec... NM_001258376.1 100% 100%
2 human 58513 EPS15L1 epidermal growth factor rec... XM_017027092.2 79.9% 79.2% (many diffs)
3 human 58513 EPS15L1 epidermal growth factor rec... NM_001258375.1 79.5% 78.8% (many diffs)
4 human 58513 EPS15L1 epidermal growth factor rec... XM_017027091.2 75.3% 74.7% (many diffs)
5 human 58513 EPS15L1 epidermal growth factor rec... XM_017027090.2 75.2% 74.6% (many diffs)
6 human 58513 EPS15L1 epidermal growth factor rec... XM_017027089.2 70.9% 70.4% (many diffs)
7 human 58513 EPS15L1 epidermal growth factor rec... NM_021235.2 69.4% 68.8% (many diffs)
8 human 58513 EPS15L1 epidermal growth factor rec... XM_017027088.2 68.6% 68% (many diffs)
9 human 58513 EPS15L1 epidermal growth factor rec... NM_001258374.3 65.9% 65.3% (many diffs)
10 human 58513 EPS15L1 epidermal growth factor rec... XM_017027087.2 65.5% 65% (many diffs)
11 human 58513 EPS15L1 epidermal growth factor rec... XM_017027086.2 64.7% 64.2% (many diffs)
12 human 58513 EPS15L1 epidermal growth factor rec... NR_047666.1 62.2% (many diffs)
13 human 58513 EPS15L1 epidermal growth factor rec... NR_047665.1 57.3% (many diffs)
14 mouse 13859 Eps15l1 epidermal growth factor rec... NM_001122832.1 86% 92.8% (many diffs)
15 mouse 13859 Eps15l1 epidermal growth factor rec... NM_001289859.1 66.9% 68.5% (many diffs)
16 mouse 13859 Eps15l1 epidermal growth factor rec... XM_017312560.1 66.9% 68.5% (many diffs)
17 mouse 13859 Eps15l1 epidermal growth factor rec... XM_006509547.3 64.7% 69.3% (many diffs)
18 mouse 13859 Eps15l1 epidermal growth factor rec... XM_006509548.3 64.7% 69.3% (many diffs)
19 mouse 13859 Eps15l1 epidermal growth factor rec... XM_017312559.1 59.7% 63.8% (many diffs)
20 mouse 13859 Eps15l1 epidermal growth factor rec... XM_006509546.3 58.3% 59.9% (many diffs)
21 mouse 13859 Eps15l1 epidermal growth factor rec... XM_006509545.3 57.3% 58.8% (many diffs)
22 mouse 13859 Eps15l1 epidermal growth factor rec... NM_007944.3 56.7% 60.7% (many diffs)
23 mouse 13859 Eps15l1 epidermal growth factor rec... XM_006509544.3 55.7% 59.6% (many diffs)
24 mouse 13859 Eps15l1 epidermal growth factor rec... XR_379032.3 53.4% (many diffs)
25 mouse 13859 Eps15l1 epidermal growth factor rec... XR_379029.3 52.1% (many diffs)
26 mouse 13859 Eps15l1 epidermal growth factor rec... XR_379030.3 50.2% (many diffs)
27 mouse 13859 Eps15l1 epidermal growth factor rec... XR_001778398.1 49.8% (many diffs)
28 mouse 13859 Eps15l1 epidermal growth factor rec... XR_379031.3 47.8% (many diffs)
29 mouse 13859 Eps15l1 epidermal growth factor rec... XM_006509550.2 27.5% 27.1% (many diffs)
30 mouse 13859 Eps15l1 epidermal growth factor rec... XR_001778401.1 26.7% (many diffs)
31 mouse 13859 Eps15l1 epidermal growth factor rec... XR_001778400.1 22.5% (many diffs)
32 mouse 13859 Eps15l1 epidermal growth factor rec... XR_001778399.1 22% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1872
ORF length:
1803
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggcggcgccg ctcatccccc tctcccagca gattcccact ggaaattcgt
121tgtatgaatc ttattacaag caggtcgatc cggcatacac agggagggtg ggggcgagtg
181aagctgcgct ttttctaaag aagtctggcc tctcggacat tatccttggg aagatatggg
241acttggccga tccagaaggt aaagggttct tggacaaaca gggtttctat gttgcactga
301gactggtggc ctgtgcacag agtggccatg aagttacctt gagcaatctg aatttgagca
361tgccaccgcc taaatttcac gacaccagca gccctctgat ggtcacaccg ccctctgcag
421aggcccactg ggctgtgagg gtggaagaaa aggccaaatt tgatgggatt tttgaaagcc
481tcttgcccat caatggtttg ctctctggag acaaagtcaa gccagtcctc atgaactcaa
541agctgcctct tgatgtcctg ggcagggtct gggacctcag tgacattgac aaggatgggc
601acttggatcg agatgagttc gctgtggcca tgcacttggt gtaccgagcc ctggagaagg
661agcccgtgcc ctccgccctg cccccgtccc tcatcccacc ctccaagaga aagaagactg
721tgttccctgg cgccgtcccc gtcctgcctg ccagcccccc accaaaagac agcctccgct
781ccacgccgtc ccacggcagc gtcagcagcc tcaacagcac agggagcctg tcccccaagc
841acagcctcaa gcaaacacag ccaacagtga actgggtggt gcccgtggca gacaagatgc
901gatttgatga gatattcctg aagaccgacc tggacctgga tggctacgtg agtggccagg
961aggtgaagga gatcttcatg cactcgggcc tcacccagaa ccttctagca cacatatggg
1021ccctggccga tacgaggcaa acggggaagt taagcaaaga ccaattcgcg ttagctatgt
1081atttcattca gcagaaggtc agtaaaggca tcgaccctcc tcaagtcctc tcgccggaca
1141tggtcccgcc ttcggagaga ggcacgcccg gcccggacag ttcaggctct ctcggctccg
1201gggagtttac tggcgtgaag gagcttgatg acatcagtca agagattgcc cagttacaaa
1261gagagaaata ttcactggaa caagacattc gagaaaagga agaggcaatc agacagaaaa
1321ccagcgaggt gcaggaatta caaaatgacc tagaccggga aacaagcagt ttgcaggagc
1381tcgaggctca gaaacaggat gctcaagacc gcctggacga gatggaccag cagaaggcca
1441agctccgaga catgctgagc gacgtccggc agaagtgcca ggatgagact cagatgatct
1501catcactgaa aacgcaaatc caatctcagg aatctgactt aaagtcccag gaagacgatc
1561tgaaccgagc caagtcggag ctgaaccgat tgcagcagga ggaaacCCAG CTGGAGCAGA
1621GCATTCAGGC TGGGCGAGTC CAGCTGGAAA CCATCATCAA GTCCCTGAAG TCAACGCAAG
1681ACGAAATCAA CCAGGCAAGG AGCAAACTTT CCCAGCTGCA TGAAAGCCGC CAGGAGGCCC
1741ACAGGAGCCT GGAGCAGTAT GACCAGGTGC TCGATGGAGC CCATGGTGCC AGCCTGACCG
1801ACCTGGCCAA CCTGAGCGAA GGCGTCTCCC TGGCAGAGAG GGGCAGTTTT GGAGCCATGG
1861TAAAGGTTGA ATTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA
1921ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT
1981CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATTC ACTGTACTAG CTTGCTTTCT
2041CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)