Construct: ORF TRCN0000475983

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009921.1_s317c1
Derived from:
ccsbBroadEn_05800
DNA Barcode:
CTCTTTATGTTCTAATTAATGAAG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ALCAM (214)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475983
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 214 ALCAM activated leukocyte cell ad... NM_001627.4 99.8% 100% 900G>A;978T>C;1266G>A
2 human 214 ALCAM activated leukocyte cell ad... NM_001243280.2 97.5% 97.5% (many diffs)
3 human 214 ALCAM activated leukocyte cell ad... NM_001243281.1 95% 95.1% 900G>A;1266G>A;1665_1666ins84
4 human 214 ALCAM activated leukocyte cell ad... NM_001243283.1 22.8% 22.4% 394_395ins23;399_400ins1327
5 mouse 11658 Alcam activated leukocyte cell ad... NM_009655.3 89.1% 92.9% (many diffs)
6 mouse 11658 Alcam activated leukocyte cell ad... NM_001331110.1 87% 90.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1818
ORF length:
1749
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggaatccaag ggggccagtt cctgccgtct gctcttctgc ctcttgatct
121ccgccaccgt cttcaggcca ggccttggat ggtatactgt aaattcagca tatggagata
181ccattatcat accttgccga cttgacgtac ctcagaatct catgtttggc aaatggaaat
241atgaaaagcc cgatggctcc ccagtattta ttgccttcag atcctctaca aagaaaagtg
301tgcagtacga cgatgtacca gaatacaaag acagattgaa cctctcagaa aactacactt
361tgtctatcag taatgcaagg atcagtgatg aaaagagatt tgtgtgcatg ctagtaactg
421aggacaacgt gtttgaggca cctacaatag tcaaggtgtt caagcaacca tctaaacctg
481aaattgtaag caaagcactg tttctcgaaa cagagcagct aaaaaagttg ggtgactgca
541tttcagaaga cagttatcca gatggcaata tcacatggta caggaatgga aaagtgctac
601atccccttga aggagcggtg gtcataattt ttaaaaagga aatggaccca gtgactcagc
661tctataccat gacttccacc ctggagtaca agacaaccaa ggctgacata caaatgccat
721tcacctgctc ggtgacatat tatggaccat ctggccagaa aacaattcat tctgaacagg
781cagtatttga tatttactat cctacagagc aggtgacaat acaagtgctg ccaccaaaaa
841atgccatcaa agaaggggat aacatcactc ttaaatgctt agggaatggc aaccctcccc
901cagaggaatt tttgttttac ttaccaggac agcccgaagg aataagaagc tcaaatactt
961acacactaac ggatgtgagg cgcaatgcaa caggagacta caagtgttcc ctgatagaca
1021aaaaaagcat gattgcttca acagccatca cagttcacta tttggatttg tccttaaacc
1081caagtggaga agtgactaga cagattggtg atgccctacc cgtgtcatgc acaatatctg
1141ctagcaggaa tgcaactgtg gtatggatga aagataacat caggcttcga tctagcccgt
1201cattttctag tcttcattat caggatgctg gaaactatgt ctgcgaaact gctctgcagg
1261aggttgaagg actaaagaaa agagagtcat tgactctcat tgtagaaggc aaacctcaaa
1321taaaaatgac aaaaaaaact gatcccagtg gactatctaa aacaataatc tgccatgtgg
1381aaggttttcc aaagccagcc attcaatgga caattactgg cagtggaagc gtcataaacc
1441aaacagagga atctccttat attaatggca ggtattatag taaaattatc atttccccTG
1501AAGAGAATGT TACATTAACT TGCACAGCAG AAAACCAACT GGAGAGAACA GTAAACTCCT
1561TGAATGTCTC TGCTATAAGT ATTCCAGAAC ACGATGAGGC AGACGAGATA AGTGATGAAA
1621ACAGAGAAAA GGTGAATGAC CAGGCAAAAC TAATTGTGGG AATCGTTGTT GGTCTCCTCC
1681TTGCTGCCCT TGTTGCTGGT GTCGTCTACT GGCTGTACAT GAAGAAGTCA AAGACTGCAT
1741CAAAACATGT AAACAAGGAC CTCGGTAATA TGGAAGAAAA CAAAAAGTTA GAAGAAAACA
1801ATCACAAAAC TGAAGCCTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA
1861TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA
1921GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACTCTTTA TGTTCTAATT
1981AATGAAGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)