Construct: ORF TRCN0000475983
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009921.1_s317c1
- Derived from:
- ccsbBroadEn_05800
- DNA Barcode:
- CTCTTTATGTTCTAATTAATGAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ALCAM (214)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475983
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 214 | ALCAM | activated leukocyte cell ad... | NM_001627.4 | 99.8% | 100% | 900G>A;978T>C;1266G>A |
| 2 | human | 214 | ALCAM | activated leukocyte cell ad... | NM_001243280.2 | 97.5% | 97.5% | (many diffs) |
| 3 | human | 214 | ALCAM | activated leukocyte cell ad... | NM_001243281.1 | 95% | 95.1% | 900G>A;1266G>A;1665_1666ins84 |
| 4 | human | 214 | ALCAM | activated leukocyte cell ad... | NM_001243283.1 | 22.8% | 22.4% | 394_395ins23;399_400ins1327 |
| 5 | mouse | 11658 | Alcam | activated leukocyte cell ad... | NM_009655.3 | 89.1% | 92.9% | (many diffs) |
| 6 | mouse | 11658 | Alcam | activated leukocyte cell ad... | NM_001331110.1 | 87% | 90.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1818
- ORF length:
- 1749
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaatccaag ggggccagtt cctgccgtct gctcttctgc ctcttgatct 121 ccgccaccgt cttcaggcca ggccttggat ggtatactgt aaattcagca tatggagata 181 ccattatcat accttgccga cttgacgtac ctcagaatct catgtttggc aaatggaaat 241 atgaaaagcc cgatggctcc ccagtattta ttgccttcag atcctctaca aagaaaagtg 301 tgcagtacga cgatgtacca gaatacaaag acagattgaa cctctcagaa aactacactt 361 tgtctatcag taatgcaagg atcagtgatg aaaagagatt tgtgtgcatg ctagtaactg 421 aggacaacgt gtttgaggca cctacaatag tcaaggtgtt caagcaacca tctaaacctg 481 aaattgtaag caaagcactg tttctcgaaa cagagcagct aaaaaagttg ggtgactgca 541 tttcagaaga cagttatcca gatggcaata tcacatggta caggaatgga aaagtgctac 601 atccccttga aggagcggtg gtcataattt ttaaaaagga aatggaccca gtgactcagc 661 tctataccat gacttccacc ctggagtaca agacaaccaa ggctgacata caaatgccat 721 tcacctgctc ggtgacatat tatggaccat ctggccagaa aacaattcat tctgaacagg 781 cagtatttga tatttactat cctacagagc aggtgacaat acaagtgctg ccaccaaaaa 841 atgccatcaa agaaggggat aacatcactc ttaaatgctt agggaatggc aaccctcccc 901 cagaggaatt tttgttttac ttaccaggac agcccgaagg aataagaagc tcaaatactt 961 acacactaac ggatgtgagg cgcaatgcaa caggagacta caagtgttcc ctgatagaca 1021 aaaaaagcat gattgcttca acagccatca cagttcacta tttggatttg tccttaaacc 1081 caagtggaga agtgactaga cagattggtg atgccctacc cgtgtcatgc acaatatctg 1141 ctagcaggaa tgcaactgtg gtatggatga aagataacat caggcttcga tctagcccgt 1201 cattttctag tcttcattat caggatgctg gaaactatgt ctgcgaaact gctctgcagg 1261 aggttgaagg actaaagaaa agagagtcat tgactctcat tgtagaaggc aaacctcaaa 1321 taaaaatgac aaaaaaaact gatcccagtg gactatctaa aacaataatc tgccatgtgg 1381 aaggttttcc aaagccagcc attcaatgga caattactgg cagtggaagc gtcataaacc 1441 aaacagagga atctccttat attaatggca ggtattatag taaaattatc atttccccTG 1501 AAGAGAATGT TACATTAACT TGCACAGCAG AAAACCAACT GGAGAGAACA GTAAACTCCT 1561 TGAATGTCTC TGCTATAAGT ATTCCAGAAC ACGATGAGGC AGACGAGATA AGTGATGAAA 1621 ACAGAGAAAA GGTGAATGAC CAGGCAAAAC TAATTGTGGG AATCGTTGTT GGTCTCCTCC 1681 TTGCTGCCCT TGTTGCTGGT GTCGTCTACT GGCTGTACAT GAAGAAGTCA AAGACTGCAT 1741 CAAAACATGT AAACAAGGAC CTCGGTAATA TGGAAGAAAA CAAAAAGTTA GAAGAAAACA 1801 ATCACAAAAC TGAAGCCTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1861 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1921 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACTCTTTA TGTTCTAATT 1981 AATGAAGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt