Construct: ORF TRCN0000475986
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011071.1_s317c1
- Derived from:
- ccsbBroadEn_09177
- DNA Barcode:
- GGCTTAACTGACACAGGATACTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EFCAB2 (84288)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475986
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84288 | EFCAB2 | EF-hand calcium binding dom... | NM_032328.3 | 99.7% | 100% | 201C>T |
| 2 | human | 84288 | EFCAB2 | EF-hand calcium binding dom... | XM_017002544.1 | 94.8% | 94.4% | (many diffs) |
| 3 | human | 84288 | EFCAB2 | EF-hand calcium binding dom... | NM_001290327.1 | 92.6% | 90.1% | (many diffs) |
| 4 | human | 84288 | EFCAB2 | EF-hand calcium binding dom... | XM_011544301.1 | 92.6% | 90.1% | (many diffs) |
| 5 | human | 84288 | EFCAB2 | EF-hand calcium binding dom... | XM_011544300.2 | 84.3% | 81.4% | (many diffs) |
| 6 | human | 84288 | EFCAB2 | EF-hand calcium binding dom... | NM_001143943.1 | 79.6% | 73.5% | (many diffs) |
| 7 | human | 84288 | EFCAB2 | EF-hand calcium binding dom... | XM_011544302.3 | 79.4% | 78.3% | (many diffs) |
| 8 | human | 84288 | EFCAB2 | EF-hand calcium binding dom... | XM_017002539.1 | 78.3% | 70.3% | (many diffs) |
| 9 | human | 84288 | EFCAB2 | EF-hand calcium binding dom... | XM_017002541.1 | 75.2% | 69.1% | (many diffs) |
| 10 | human | 84288 | EFCAB2 | EF-hand calcium binding dom... | XM_017002540.1 | 75% | 69.1% | (many diffs) |
| 11 | human | 84288 | EFCAB2 | EF-hand calcium binding dom... | XM_017002542.2 | 74.4% | 68% | (many diffs) |
| 12 | human | 84288 | EFCAB2 | EF-hand calcium binding dom... | XM_017002538.1 | 73.7% | 70.2% | (many diffs) |
| 13 | human | 84288 | EFCAB2 | EF-hand calcium binding dom... | XM_017002543.1 | 71% | 68.6% | (many diffs) |
| 14 | human | 84288 | EFCAB2 | EF-hand calcium binding dom... | XM_017002545.1 | 28.8% | 24.1% | (many diffs) |
| 15 | human | 84288 | EFCAB2 | EF-hand calcium binding dom... | NR_026587.1 | 9.6% | (many diffs) | |
| 16 | mouse | 68226 | Efcab2 | EF-hand calcium binding dom... | NM_026626.2 | 85.8% | 84.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 552
- ORF length:
- 486
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggacgagaag gacagggaag agataatagt agcagaattt cacaaaaaaa 121 tcaaagaggc atttgaagtc tttgaccatg agtcgaataa tacagtggat gtgagagaga 181 ttggaacaat tatcaggtca ttaggatgct gtcctacgga aggagagctg catgatctga 241 ttgcagaggt agaggaagaa gaacctactG GATACATTCG ATTCGAAAAA TTTCTTCCGG 301 TGATGACAGA AATACTACTA GAAAGAAAAT ACAGACCAAT TCCAGAAGAT GTCCTTCTTC 361 GAGCTTTTGA GGTTTTAGAT TCAGCTAAAC GTGGGTTTCT TACTAAGGAC GAGCTGATCA 421 AGTATATGAC TGAAGAAGGT GAGCCTTTTT CTCAAGAGGA AATGGAAGAA ATGTTGTCTG 481 CTGCAATTGA TCCAGAATCA AATTCAATTA ATTACAAGGA CTATATAACA ATGATGGTGA 541 TAGATGAAAA TTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 601 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 661 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGGC TTAACTGACA CAGGATACTT 721 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t