Construct: ORF TRCN0000476019
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000916.1_s317c1
- Derived from:
- ccsbBroadEn_06163
- DNA Barcode:
- TGCTCGTCATGAAACGAGGGTATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ENPEP (2028)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476019
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2028 | ENPEP | glutamyl aminopeptidase | NM_001977.4 | 99.9% | 99.8% | 638A>G |
| 2 | human | 2028 | ENPEP | glutamyl aminopeptidase | XM_017007877.1 | 62.5% | 62.5% | 0_1ins1074 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2940
- ORF length:
- 2871
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaactttgcg gagagagagg gctctaagag atactgcatt caaacgaaac 121 atgtggccat tctctgtgcg gtggtggtgg gtgtaggatt aatagtggga cttgccgtgg 181 gcttgaccag atcgtgtgac tccagcgggg acggcgggcc gggcactgcg ccagctcctt 241 cccacctgcc ttcttccacg gccagcccct caggtcctcc tgcccaggac caggacatct 301 gcccggccag tgaggatgag agcggacagt ggaaaaactt tcgactgccg gacttcgtca 361 acccagtcca ctacgacctg cacgtgaagc ccctgttgga ggaggacacc tacacgggca 421 ccgtgagcat ctccatcaac ctgagcgctc ccacccggta cctgtggctg cacctccggg 481 agaccaggat cacccggctc ccggagctga agaggccctc tggggaccag gtgcaagtcc 541 ggaggtgttt cgagtacaaa aagcaggagt acgtggtggt cgaggcggag gaagagctta 601 cccccagcag tggagatggc ctgtatctcc tgaccatgga gttcgccggc tggctgaacg 661 gctccctcgt gggattttat agaaccacct acacggagaa cggacgagtc aagagcatag 721 tggccaccga tcatgaacca acagatgcca ggaaatcttt tccttgtttt gatgagccca 781 acaaaaaggc aacttataca atatctatca cccatcccaa agaatacgga gcactttcaa 841 atatgccagt ggcgaaagaa gagtcagtgg atgataaatg gactcgaaca acttttgaga 901 agtctgtccc catgagcacg tacctggtgt gctttgctgt acatcaattt gactctgtaa 961 agagaatatc aaatagtgga aaacctctta caatttatgt ccagccagag caaaagcaca 1021 cagccgaata tgctgcaaac ataactaaaa gtgtgtttga ttattttgaa gaatactttg 1081 ctatgaatta ttctcttcct aaattagata aaatcgctat tccagatttt ggcactggtg 1141 ccatggagaa ctggggactc atcacgtaca gagaaacgaa cctgctttat gaccctaagg 1201 aatcagcctc atcaaaccaa cagagggtgg ccactgtggt tgcccatgaa cttgtgcatc 1261 agtggtttgg aaatattgtg accatggact ggtgggaaga cttgtggcta aatgaaggat 1321 ttgcttcttt ctttgagttt ctgggagtaa accatgcaga aacagactgg caaatgcgtg 1381 accaaatgtt acttgaagat gtattacctg ttcaagagga tgattctttg atgtcttcgc 1441 atccaattat tgtgactgtg acaacccctg atgaaataac atctgttttt gatggaatat 1501 cctatagcaa gggatcttct attttgagaa tgcttgaaga ctggataaaa ccagagaatt 1561 ttcaaaaagg atgtcagatg tacttggaaa aataccaatt caagaatgca aaaacttctg 1621 atttttgggc agcactggaa gaggcaagta ggctaccagt gaaagaagta atggacacct 1681 ggaccagaca gatgggttat cctgtgctta acgtgaacgg tgtcaagaac atcacacaga 1741 aacgcttttt gttggaccca agagctaacc cttctcagcc cccttcagat cttggttata 1801 catggaatat cccagttaaa tggactgaag ataatataac aagcagtgtg ttatttaata 1861 ggtcagaaaa agaaggaatc actttgaact cctctaatcc tagtggaaat gcttttctca 1921 aaataaaccc agatcatatt gggttttatc gtgtaaatta tgaagtagca acttgggact 1981 cgatagctac agcgctctcc ttgaaccaca agacattttc ttcagcagat cgtgcaagtc 2041 ttattgatga tgcttttgcc ttggcaagag ctcaacttct agattataag gtggctttga 2101 acttgaccaa gtatctcaaa agggaagaga attttttacc atggcagaga gtaatttcag 2161 ctgtaaccta catcattagc atgtttgaag atgataaaga gctatatcct atgattgagg 2221 aatacttcca aggtcaagtg aagcctattg cagattctct gggatggaat gatgctggag 2281 accatgtcac aaagttactc cgttcctccg tgttagggtt tgcgtgcaag atgggagaca 2341 gagaagcctt gaacaatgct tcctcgttat ttgagcagtg gctaaatggg actgtaagcc 2401 ttcccgtaaa tctcaggctt ctggtgtatc ggtatgggat gcagaactct ggcaatgaga 2461 tttcatggaa ctacactctt gagcaatacc agaaaacttc attagctcaa gaaaaagaaa 2521 aactgctgta tggattagca tcagtgaaga acgttactct tttgtcaagg tatttggatt 2581 tgctcaagga cacgaaCCTT ATTAAAACTC AGGATGTGTT TACAGTCATT CGATATATCT 2641 CATATAACAG CTATGGGAAG AACATGGCCT GGAATTGGAT ACAACTCAAC TGGGACTATC 2701 TAGTCAACAG ATATACACTC AATAACAGAA ACCTTGGCCG AATTGTCACA ATAGCAGAGC 2761 CATTCAACAC TGAACTGCAA CTGTGGCAGA TGGAGAGCTT TTTTGCAAAA TATCCACAAG 2821 CTGGAGCAGG AGAAAAACCT AGGGAACAAG TGCTGGAAAC AGTGAAAAAC AATATAGAGT 2881 GGCTAAAACA ACATAGAAAC ACCATCAGAG AATGGTTTTT TAATTTACTT GAGAGTGGTT 2941 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 3001 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 3061 TTTATATATC TTGTGGAAAG GACGATGCTC GTCATGAAAC GAGGGTATCA CGCGTTAAGT 3121 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt