Construct: ORF TRCN0000476019

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000916.1_s317c1
Derived from:
ccsbBroadEn_06163
DNA Barcode:
TGCTCGTCATGAAACGAGGGTATC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ENPEP (2028)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000476019
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2028 ENPEP glutamyl aminopeptidase NM_001977.4 99.9% 99.8% 638A>G
2 human 2028 ENPEP glutamyl aminopeptidase XM_017007877.1 62.5% 62.5% 0_1ins1074
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2940
ORF length:
2871
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gaactttgcg gagagagagg gctctaagag atactgcatt caaacgaaac
121atgtggccat tctctgtgcg gtggtggtgg gtgtaggatt aatagtggga cttgccgtgg
181gcttgaccag atcgtgtgac tccagcgggg acggcgggcc gggcactgcg ccagctcctt
241cccacctgcc ttcttccacg gccagcccct caggtcctcc tgcccaggac caggacatct
301gcccggccag tgaggatgag agcggacagt ggaaaaactt tcgactgccg gacttcgtca
361acccagtcca ctacgacctg cacgtgaagc ccctgttgga ggaggacacc tacacgggca
421ccgtgagcat ctccatcaac ctgagcgctc ccacccggta cctgtggctg cacctccggg
481agaccaggat cacccggctc ccggagctga agaggccctc tggggaccag gtgcaagtcc
541ggaggtgttt cgagtacaaa aagcaggagt acgtggtggt cgaggcggag gaagagctta
601cccccagcag tggagatggc ctgtatctcc tgaccatgga gttcgccggc tggctgaacg
661gctccctcgt gggattttat agaaccacct acacggagaa cggacgagtc aagagcatag
721tggccaccga tcatgaacca acagatgcca ggaaatcttt tccttgtttt gatgagccca
781acaaaaaggc aacttataca atatctatca cccatcccaa agaatacgga gcactttcaa
841atatgccagt ggcgaaagaa gagtcagtgg atgataaatg gactcgaaca acttttgaga
901agtctgtccc catgagcacg tacctggtgt gctttgctgt acatcaattt gactctgtaa
961agagaatatc aaatagtgga aaacctctta caatttatgt ccagccagag caaaagcaca
1021cagccgaata tgctgcaaac ataactaaaa gtgtgtttga ttattttgaa gaatactttg
1081ctatgaatta ttctcttcct aaattagata aaatcgctat tccagatttt ggcactggtg
1141ccatggagaa ctggggactc atcacgtaca gagaaacgaa cctgctttat gaccctaagg
1201aatcagcctc atcaaaccaa cagagggtgg ccactgtggt tgcccatgaa cttgtgcatc
1261agtggtttgg aaatattgtg accatggact ggtgggaaga cttgtggcta aatgaaggat
1321ttgcttcttt ctttgagttt ctgggagtaa accatgcaga aacagactgg caaatgcgtg
1381accaaatgtt acttgaagat gtattacctg ttcaagagga tgattctttg atgtcttcgc
1441atccaattat tgtgactgtg acaacccctg atgaaataac atctgttttt gatggaatat
1501cctatagcaa gggatcttct attttgagaa tgcttgaaga ctggataaaa ccagagaatt
1561ttcaaaaagg atgtcagatg tacttggaaa aataccaatt caagaatgca aaaacttctg
1621atttttgggc agcactggaa gaggcaagta ggctaccagt gaaagaagta atggacacct
1681ggaccagaca gatgggttat cctgtgctta acgtgaacgg tgtcaagaac atcacacaga
1741aacgcttttt gttggaccca agagctaacc cttctcagcc cccttcagat cttggttata
1801catggaatat cccagttaaa tggactgaag ataatataac aagcagtgtg ttatttaata
1861ggtcagaaaa agaaggaatc actttgaact cctctaatcc tagtggaaat gcttttctca
1921aaataaaccc agatcatatt gggttttatc gtgtaaatta tgaagtagca acttgggact
1981cgatagctac agcgctctcc ttgaaccaca agacattttc ttcagcagat cgtgcaagtc
2041ttattgatga tgcttttgcc ttggcaagag ctcaacttct agattataag gtggctttga
2101acttgaccaa gtatctcaaa agggaagaga attttttacc atggcagaga gtaatttcag
2161ctgtaaccta catcattagc atgtttgaag atgataaaga gctatatcct atgattgagg
2221aatacttcca aggtcaagtg aagcctattg cagattctct gggatggaat gatgctggag
2281accatgtcac aaagttactc cgttcctccg tgttagggtt tgcgtgcaag atgggagaca
2341gagaagcctt gaacaatgct tcctcgttat ttgagcagtg gctaaatggg actgtaagcc
2401ttcccgtaaa tctcaggctt ctggtgtatc ggtatgggat gcagaactct ggcaatgaga
2461tttcatggaa ctacactctt gagcaatacc agaaaacttc attagctcaa gaaaaagaaa
2521aactgctgta tggattagca tcagtgaaga acgttactct tttgtcaagg tatttggatt
2581tgctcaagga cacgaaCCTT ATTAAAACTC AGGATGTGTT TACAGTCATT CGATATATCT
2641CATATAACAG CTATGGGAAG AACATGGCCT GGAATTGGAT ACAACTCAAC TGGGACTATC
2701TAGTCAACAG ATATACACTC AATAACAGAA ACCTTGGCCG AATTGTCACA ATAGCAGAGC
2761CATTCAACAC TGAACTGCAA CTGTGGCAGA TGGAGAGCTT TTTTGCAAAA TATCCACAAG
2821CTGGAGCAGG AGAAAAACCT AGGGAACAAG TGCTGGAAAC AGTGAAAAAC AATATAGAGT
2881GGCTAAAACA ACATAGAAAC ACCATCAGAG AATGGTTTTT TAATTTACTT GAGAGTGGTT
2941TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
3001GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
3061TTTATATATC TTGTGGAAAG GACGATGCTC GTCATGAAAC GAGGGTATCA CGCGTTAAGT
3121Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)