Construct: ORF TRCN0000476023
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014477.1_s317c1
- Derived from:
- ccsbBroadEn_05059
- DNA Barcode:
- AAGCTCTACACTTCCGCTACTCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MB21D2 (151963)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476023
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 151963 | MB21D2 | Mab-21 domain containing 2 | NM_178496.4 | 100% | 100% | |
| 2 | mouse | 239796 | Mb21d2 | Mab-21 domain containing 2 | NM_177718.3 | 89.6% | 98.5% | (many diffs) |
| 3 | mouse | 239796 | Mb21d2 | Mab-21 domain containing 2 | XM_006522115.3 | 79.6% | 79.3% | (many diffs) |
| 4 | mouse | 239796 | Mb21d2 | Mab-21 domain containing 2 | XM_006522116.3 | 77.4% | 84.8% | (many diffs) |
| 5 | mouse | 239796 | Mb21d2 | Mab-21 domain containing 2 | XM_006522117.1 | 77.2% | 85.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1542
- ORF length:
- 1473
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaagatggcg gctcccaccg ccaacaaggc agcctccctg ggctgtaaca 121 acaagcctgc gttcccggag ctggatttca ggtcgggagc tcgggtggag gaattgaaca 181 aactcatcca agaatttacg aagcacgacc agcgggaata cgacgaccag agagcgctgg 241 agattcacac agccaaggat ttcatctttt ccatgctggg aatggtgcaa aagctggacc 301 aaaagcttcc agtggctaat gaatacctgt tgctctctgg aggtgtccgg gaaggcgtgg 361 tggacctgga cttagatgag cttaatgtct atgcccgggg tactgactat gatatggact 421 ttaccctctt ggtgccagcc ctcaagctgc atgaccgtaa tcagcctgtg acactcgaca 481 tgcgccactc agccttgtgc cactcttggc tgagccttcg gctctttgat gaggggacaa 541 tcagtaaatg gaaagactgc tgcaccattg tagatcacat caatggtgcc accaactact 601 tcttctcacc taccaaagtg gctgactggt tctatgactc tatcagcatt gtcctatcag 661 aaatacagaa gaaaccccag cgagggatgc caaaggtaga aaaggtggaa aaaaatggga 721 ccatcatctc catcattctg ggtgtaggga gtagtcgcat gttgtatgat attgtccctg 781 tggtatcttt caaaggttgg cctgcagtgg cccagagctg gctcatggag aaccactttt 841 gggatgggaa gattactgag gaagaggtca tcagtgggtt ttacttggtg cctgcttgct 901 cctacaaggg taagaaggac aatgaatggc ggctgtcctt tgccaggagc gaggtgcagt 961 tgaagaagtg catctccagc agcctcatgc aggcctatca ggcctgcaaa gccatcatca 1021 ttaaactgct gtcccggccc aaggctatta gcccctatca cctgcggagc atgatgctct 1081 gggcctgcga cagacttcct gccaactact tggctcaaga agactatgca gcccactttt 1141 tgctgggcct catcgatgac ctgcaacact gtctggtcaa caagatgtgc cccaattatt 1201 tcatccctca gtgcaacatg ctggaacatc tgtctgagga gacagtcatg cttcacgccc 1261 ggaagctgtc ctctgtgcgc TCAGACCCGG CAGAGCACTT GCGCACCGCC ATTGAGCATG 1321 TCAAGGCAGC CAACCGGCTG ACACTGGAGC TCCAGAGGCG AGGTAGCACC ACCAGCATCC 1381 CCTCTCCACA GTCTGACGGA GGGGACCCCA ACCAGCCTGA TGACCGTTTG GCAAAAAAAC 1441 TGCAGCAGCT AGTGACTGAG AACCCGGGAA AGTCAATCTC TGTCTTTATC AATCCTGACG 1501 ATGTCACAAG GCCCCATTTC AGAATTGATG ACAAATTTTT CTTGCCAACT TTCTTGTACA 1561 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1621 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1681 AGGACGAAAG CTCTACACTT CCGCTACTCG AACGCGTTAA GTCgacaatc aacctctgga 1741 ttacaaaatt tgtgaaagat t