Construct: ORF TRCN0000476039
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011273.1_s317c1
- Derived from:
- ccsbBroadEn_13099
- DNA Barcode:
- CAAACCTATGCTATCACAATCCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM266 (123591)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476039
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 123591 | TMEM266 | transmembrane protein 266 | XM_005254160.3 | 99.8% | 99.4% | 620G>A;728C>T |
| 2 | human | 123591 | TMEM266 | transmembrane protein 266 | XM_017021916.1 | 99.8% | 99.4% | 620G>A;728C>T |
| 3 | human | 123591 | TMEM266 | transmembrane protein 266 | XM_017021917.1 | 99.8% | 99.4% | 620G>A;728C>T |
| 4 | human | 123591 | TMEM266 | transmembrane protein 266 | NM_152335.3 | 65.2% | 64.9% | 1_552del;1172G>A;1280C>T |
| 5 | human | 123591 | TMEM266 | transmembrane protein 266 | XM_017021915.1 | 65.2% | 64.9% | 1_552del;1172G>A;1280C>T |
| 6 | mouse | 244886 | Tmem266 | transmembrane protein 266 | XM_006511162.1 | 72% | 72% | (many diffs) |
| 7 | mouse | 244886 | Tmem266 | transmembrane protein 266 | XM_006511163.3 | 72% | 72% | (many diffs) |
| 8 | mouse | 244886 | Tmem266 | transmembrane protein 266 | XM_006511164.1 | 72% | 72% | (many diffs) |
| 9 | mouse | 244886 | Tmem266 | transmembrane protein 266 | XM_006511159.1 | 62.9% | 62.9% | (many diffs) |
| 10 | mouse | 244886 | Tmem266 | transmembrane protein 266 | XM_006511160.3 | 62.9% | 62.9% | (many diffs) |
| 11 | mouse | 244886 | Tmem266 | transmembrane protein 266 | XM_006511161.2 | 62.9% | 62.9% | (many diffs) |
| 12 | mouse | 244886 | Tmem266 | transmembrane protein 266 | NM_172923.3 | 55.3% | 55.3% | (many diffs) |
| 13 | mouse | 244886 | Tmem266 | transmembrane protein 266 | XM_011242746.2 | 33.2% | 32.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1110
- ORF length:
- 1041
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtggcatcc actgtggcca atggacccag gagcccctgg gacgccatca 121 gcctcatcat catgctccgg atctggaggg tgaagagggt cattgatgcc tacgtcctgc 181 cagtgaagct ggagatggag atggttatcc agcagtacga gaaggccaag gtcatccaag 241 acgagcagct ggagaggctg acgcagatct gtcaggagca agggtttgag atccggcagc 301 tgcgcgcgca cctggcgcag caggacctgg acctggctgc cgagcgcgaa gcggcgctcc 361 aggccccgca cgtgctcagc cagccgcgca gccgcttcaa agtgttggag gccggcacgt 421 gggacgagga gacggcggcc gagagcgtcg tggaggagct gcagccctcg caagaagcca 481 cgatgaagga cgacatgaac agctacatca gtcagtatta caatgggccc agcagtgaca 541 gcggtgtccc agagccagct gtgtgtatgg tcaccacggc cgcaatagac attcaccagc 601 ccaacatctc ctcggacctc ttctctctgg acatgcccct caaactcggc ggtaatggca 661 ccagcgccac ctcggagagt gcctcccaca gctcagtcac ccgggcccag agtgacagca 721 gccagacgct gggctcctcc atggactgca gcactgcccg cgaggagccg tcctctgagc 781 ccggcccttc tcccctgccg ctgccatccc agcagcaggt gGAGGAGGCC ACAGTCCAGG 841 ACCTGCTGTC CTCCCTGTCG GAGGACCCCT GCCCTTCCCA GAAGGCCTTG GACCCAGCCC 901 CCCTCGCCCG GCCCAGCCCA GCGGGCTCGG CCCAAACCAG CCCCGAGCTG GAACACAGGG 961 TAAGTCTGTT CAACCAGAAG AACCAGGAGG GCTTCACTGT CTTTCAGATC AGGCCTGTCA 1021 TCCACTTCCA GCCCACTGTG CCCATGCTGG AGGACAAGTT CAGATCTTTG GAATCCAAAG 1081 AGCAAAAGCT GCACAGGGTC CCTGAGGCCT TGCCAACTTT CTTGTACAAA GTGGTTGATA 1141 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1201 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACAAAC 1261 CTATGCTATC ACAATCCCTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1321 tgaaagatt