Construct: ORF TRCN0000476041
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009169.1_s317c1
- Derived from:
- ccsbBroadEn_08475
- DNA Barcode:
- ATCCAGATAGCGCTCACCACGGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HAUS2 (55142)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476041
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55142 | HAUS2 | HAUS augmin like complex su... | NM_018097.3 | 99.8% | 99.5% | 694A>T |
| 2 | human | 55142 | HAUS2 | HAUS augmin like complex su... | NM_001130447.2 | 86.6% | 86.3% | 90_91ins93;601A>T |
| 3 | human | 55142 | HAUS2 | HAUS augmin like complex su... | NM_001323631.2 | 59.8% | 59.5% | 0_1ins282;412A>T |
| 4 | human | 55142 | HAUS2 | HAUS augmin like complex su... | NM_001323632.2 | 59.8% | 59.5% | 0_1ins282;412A>T |
| 5 | human | 55142 | HAUS2 | HAUS augmin like complex su... | XM_017022396.1 | 59.8% | 59.5% | 0_1ins282;412A>T |
| 6 | human | 55142 | HAUS2 | HAUS augmin like complex su... | XM_017022397.1 | 59.8% | 59.5% | 0_1ins282;412A>T |
| 7 | human | 55142 | HAUS2 | HAUS augmin like complex su... | NM_001323630.2 | 44.1% | 43.8% | 0_1ins393;301A>T |
| 8 | human | 55142 | HAUS2 | HAUS augmin like complex su... | NM_001323629.1 | 35.6% | 38.9% | 256_344del;372_373ins422 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 771
- ORF length:
- 705
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttcgcatggc cgctgccaac ccgtgggacc cggcgtccgc gcctaacggc gctgggctag 121 tgctaggcca cttcatagct tcggggatgg tcaatcagga gatgttaaac atgtctaaga 181 aaacagtttc ttgttttgtg aacttcacca gactacagca gatcacaaat attcaagctg 241 aaatctacca gaaaaacctg gaaattgaac tcctgaaact agaaaaagat acagcagatg 301 ttgttcatcc tttctttttg gctcagaagt gtcatactct gcaaagcatg aataatcatt 361 tggaagcagt gctgaaagag aagagatccc ttaggcaaag actgttgaaa cccatgtgcc 421 aggaaaactt acctattgaa gctgtttatc acagatatat ggtacatttg ctggagttGG 481 CTGTGACTTT CATTGAGAGA TTAGAAACCC ACCTTGAAAC AATTAGAAAT ATTCCTCATT 541 TAGCTGCAAA TCTAAAGAAA ATGAACCAGG CTTTAGCAAA GATGGATATA TTGGTGACTG 601 AGACAGAAGA ACTGGCAGAG AATATACTCA AGTGGCGTAA ACAACAAAAC GAAGTTTCGT 661 CTTGTATCCC CAAAATATTA GCTGAAGAAA GTTATCTTTA TAAACATGAT ATTATAATGC 721 CTCCTTTACC TTTTACTTCT AAAGTTCATG TCCAAACTTT TAATGCCAAG TACCCAACTT 781 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 841 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 901 CTTGTGGAAA GGACGAATCC AGATAGCGCT CACCACGGAA ACGCGTTAAG TCgacaatca 961 acctctggat tacaaaattt gtgaaagatt