Construct: ORF TRCN0000476092

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011921.1_s317c1
Derived from:
ccsbBroadEn_06582
DNA Barcode:
TTGCGCCCGCGTTTGCCTGTCGCC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MGST1 (4257)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000476092
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4257 MGST1 microsomal glutathione S-tr... NM_001260511.1 100% 100%
2 human 4257 MGST1 microsomal glutathione S-tr... NM_020300.5 100% 100%
3 human 4257 MGST1 microsomal glutathione S-tr... NM_145764.2 100% 100%
4 human 4257 MGST1 microsomal glutathione S-tr... NM_145791.2 100% 100%
5 human 4257 MGST1 microsomal glutathione S-tr... NM_145792.2 100% 100%
6 human 4257 MGST1 microsomal glutathione S-tr... NM_001260512.1 61.1% 46.7% (many diffs)
7 human 4257 MGST1 microsomal glutathione S-tr... XM_006719079.1 59.8% 46.2% (many diffs)
8 human 4257 MGST1 microsomal glutathione S-tr... NM_001267598.2 53.9% 50.9% (many diffs)
9 human 4257 MGST1 microsomal glutathione S-tr... NR_048545.1 39.1% 1_90del;214_215ins95;461_849del
10 human 4257 MGST1 microsomal glutathione S-tr... NR_048547.1 37.1% 1_143del;267_268ins95;514_902del
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
531
ORF length:
465
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggt tgacctcacc caggtaatgg atgatgaagt attcatggct tttgcatccT
121ATGCAACAAT TATTCTTTCA AAAATGATGC TTATGAGTAC TGCAACTGCA TTCTATAGAT
181TGACAAGAAA GGTTTTTGCC AATCCAGAAG ACTGTGTAGC ATTTGGCAAA GGAGAAAATG
241CCAAGAAGTA TCTTCGAACA GATGACAGAG TAGAACGTGT ACGCAGAGCC CACCTGAATG
301ACCTTGAAAA TATTATTCCA TTTCTTGGAA TTGGCCTCCT GTATTCCTTG AGTGGTCCCG
361ACCCCTCTAC AGCCATCCTG CACTTCAGAC TATTTGTCGG AGCACGGATC TACCACACCA
421TTGCATATTT GACACCCCTT CCCCAGCCAA ATAGAGCTTT GAGTTTTTTT GTTGGATATG
481GAGTTACTCT TTCCATGGCT TACAGGTTGC TGAAAAGTAA ATTGTACCTG TACCCAACTT
541TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
601CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
661CTTGTGGAAA GGACGATTGC GCCCGCGTTT GCCTGTCGCC ACGCGTTAAG TCgacaatca
721acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)