Construct: ORF TRCN0000476092
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011921.1_s317c1
- Derived from:
- ccsbBroadEn_06582
- DNA Barcode:
- TTGCGCCCGCGTTTGCCTGTCGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MGST1 (4257)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476092
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4257 | MGST1 | microsomal glutathione S-tr... | NM_001260511.1 | 100% | 100% | |
| 2 | human | 4257 | MGST1 | microsomal glutathione S-tr... | NM_020300.5 | 100% | 100% | |
| 3 | human | 4257 | MGST1 | microsomal glutathione S-tr... | NM_145764.2 | 100% | 100% | |
| 4 | human | 4257 | MGST1 | microsomal glutathione S-tr... | NM_145791.2 | 100% | 100% | |
| 5 | human | 4257 | MGST1 | microsomal glutathione S-tr... | NM_145792.2 | 100% | 100% | |
| 6 | human | 4257 | MGST1 | microsomal glutathione S-tr... | NM_001260512.1 | 61.1% | 46.7% | (many diffs) |
| 7 | human | 4257 | MGST1 | microsomal glutathione S-tr... | XM_006719079.1 | 59.8% | 46.2% | (many diffs) |
| 8 | human | 4257 | MGST1 | microsomal glutathione S-tr... | NM_001267598.2 | 53.9% | 50.9% | (many diffs) |
| 9 | human | 4257 | MGST1 | microsomal glutathione S-tr... | NR_048545.1 | 39.1% | 1_90del;214_215ins95;461_849del | |
| 10 | human | 4257 | MGST1 | microsomal glutathione S-tr... | NR_048547.1 | 37.1% | 1_143del;267_268ins95;514_902del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 531
- ORF length:
- 465
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt tgacctcacc caggtaatgg atgatgaagt attcatggct tttgcatccT 121 ATGCAACAAT TATTCTTTCA AAAATGATGC TTATGAGTAC TGCAACTGCA TTCTATAGAT 181 TGACAAGAAA GGTTTTTGCC AATCCAGAAG ACTGTGTAGC ATTTGGCAAA GGAGAAAATG 241 CCAAGAAGTA TCTTCGAACA GATGACAGAG TAGAACGTGT ACGCAGAGCC CACCTGAATG 301 ACCTTGAAAA TATTATTCCA TTTCTTGGAA TTGGCCTCCT GTATTCCTTG AGTGGTCCCG 361 ACCCCTCTAC AGCCATCCTG CACTTCAGAC TATTTGTCGG AGCACGGATC TACCACACCA 421 TTGCATATTT GACACCCCTT CCCCAGCCAA ATAGAGCTTT GAGTTTTTTT GTTGGATATG 481 GAGTTACTCT TTCCATGGCT TACAGGTTGC TGAAAAGTAA ATTGTACCTG TACCCAACTT 541 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 601 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 661 CTTGTGGAAA GGACGATTGC GCCCGCGTTT GCCTGTCGCC ACGCGTTAAG TCgacaatca 721 acctctggat tacaaaattt gtgaaagatt