Construct: ORF TRCN0000476105
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006145.1_s317c1
- Derived from:
- ccsbBroadEn_02565
- DNA Barcode:
- GGGCCTCACCTTAGTTTAGCCCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RALBP1 (10928)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476105
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10928 | RALBP1 | ralA binding protein 1 | NM_006788.4 | 100% | 100% | |
| 2 | mouse | 19765 | Ralbp1 | ralA binding protein 1 | NM_001198949.1 | 87.5% | 91.7% | (many diffs) |
| 3 | mouse | 19765 | Ralbp1 | ralA binding protein 1 | NM_009067.5 | 87.5% | 91.7% | (many diffs) |
| 4 | mouse | 19765 | Ralbp1 | ralA binding protein 1 | XM_006523915.3 | 81.4% | 85% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2031
- ORF length:
- 1965
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac tgagtgcttc ctgcccccca ccagcagccc cagtgaacac cgcagggtgg 121 agcatggcag cgggcttacc cggaccccca gctctgaaga gatcagccct actaagtttc 181 ctggattgta ccgcactggc gagccctcac ctccccatga catcctccat gagcctcctg 241 atgtagtgtc tgatgatgag aaagatcatg ggaagaaaaa agggaaattt aagaaaaagg 301 aaaagaggac tgaaggctat gcagcctttc aggaagatag ctctggagat gaggcagaaa 361 gtccttctaa aatgaagagg tccaagggaa tccatgtttt caagaagccc agcttttcta 421 aaaagaagga aaaggatttt aaaataaaag agaaacccaa agaagaaaag cataaagaag 481 aaaagcacaa agaagaaaaa cataaagaga agaagtcaaa agacttgaca gcagctgatg 541 ttgttaaaca gtggaaggaa aagaagaaaa agaaaaagcc aattcaggag ccagaggtgc 601 ctcagattga tgttccaaat ctcaaaccca tttttggaat tcctttggct gatgcagtag 661 agaggaccat gatgtatgat ggcattcggc tgccagccgt tttccgtgaa tgtatagatt 721 acgtagagaa gtatggcatg aagtgtgaag gcatctacag agtatcagga attaaatcaa 781 aggtggatga gctaaaagca gcctatgacc gggaggagtc tacaaacttg gaagactatg 841 agcctaacac tgtagccagt ttgctgaagc agtatttgcg agaccttcca gagaatttgc 901 ttaccaaaga gcttatgccc agatttgaag aggcttgtgg gaggaccacg gagactgaga 961 aagtgcagga attccagcgt ttactcaaag aactgccaga atgtaactat cttctgattt 1021 cttggctcat tgtgcacatg gaccatgtca ttgcaaagga actggaaaca aaaatgaata 1081 tacagaacat ttctatagtg ctcagcccaa ctgtgcagat cagcaatcga gtcctgtatg 1141 tgtttttcac acatgtgcaa gaactctttg gaaatgtggt actaaagcaa gtgatgaaac 1201 ctctgcgatg gtctaacatg gccacgatgc ccacgctgcc agagacccag gcgggcatca 1261 aggaggagat caggagacag gagtttcttt tgaattgttt acatcgagat ctgcagggtg 1321 ggataaagga tttgtctaaa gaagaaagat tatgggaagt acaaagaatt ttgacagccc 1381 tcaaaagaaa actgagagaa gctaaaagac aggagtgtga aaccaagatt gcacaagaga 1441 tagccagtct ttcaaaagag gatgtttcca aagaagagat gaatgaaaat gaagaagtta 1501 taaatattct ccttgctcag gagaatgaga tcctgactga acaggaggag ctcctggcca 1561 tggagcagtt tctgcgccgg cagattgcct cagaaaaaga agagattgaa cgcctcagag 1621 ctgagattgc tgaaattcag agtcgccagc agcacggccg aagtgagact gaggagtact 1681 ccTCCGAGAG CGAGAGCGAG AGTGAGGATG AGGAGGAGCT GCAGATCATT CTGGAAGACT 1741 TACAGAGACA GAACGAAGAG CTGGAAATAA AGAACAATCA TTTGAATCAA GCAATTCATG 1801 AGGAGCGCGA GGCCATCATC GAGCTGCGCG TGCAGCTGCG GCTGCTCCAG ATGCAGCGAG 1861 CCAAGGCCGA GCAGCAGGCG CAGGAGGACG AGGAGCCTGA GTGGCGCGGG GGTGCCGTCC 1921 AGCCGCCCAG AGACGGCGTC CTTGAGCCAA AAGCAGCTAA AGAGCAGCCA AAGGCAGGCA 1981 AGGAGCCGGC AAAGCCATCG CCCAGCAGGG ATAGGAAGGA GACGTCCATC TGCCCAACTT 2041 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2101 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2161 CTTGTGGAAA GGACGAGGGC CTCACCTTAG TTTAGCCCCG ACGCGTTAAG TCgacaatca 2221 acctctggat tacaaaattt gtgaaagatt