Construct: ORF TRCN0000476112
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007484.1_s317c1
- Derived from:
- ccsbBroadEn_07495
- DNA Barcode:
- AGCCATAGGTCTTTCACCCCAACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GINS1 (9837)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476112
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9837 | GINS1 | GINS complex subunit 1 | NM_021067.5 | 99.8% | 99.4% | 289G>A |
| 2 | human | 9837 | GINS1 | GINS complex subunit 1 | XM_017028162.1 | 81.9% | 81.6% | 0_1ins105;184G>A |
| 3 | human | 9837 | GINS1 | GINS complex subunit 1 | XR_937180.2 | 48.6% | (many diffs) | |
| 4 | human | 9837 | GINS1 | GINS complex subunit 1 | XR_244227.4 | 37.9% | (many diffs) | |
| 5 | human | 9837 | GINS1 | GINS complex subunit 1 | NR_134574.1 | 17.1% | (many diffs) | |
| 6 | mouse | 69270 | Gins1 | GINS complex subunit 1 (Psf... | NM_027014.1 | 88.2% | 92.8% | (many diffs) |
| 7 | mouse | 69270 | Gins1 | GINS complex subunit 1 (Psf... | NM_001163476.1 | 71.3% | 72.4% | (many diffs) |
| 8 | mouse | 69270 | Gins1 | GINS complex subunit 1 (Psf... | XM_006500133.2 | 66.8% | 70.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 654
- ORF length:
- 588
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttagcatgtt ctgcgaaaaa gccatggaac tgatccgcga gctgcatcgc gcgcccgaag 121 ggcaactgcc tgccttcaac gaggatggac tcagacaagt tctggaggag atgaaagctt 181 tgtatgaaca aaaccagtct gatgtgaatg aagcaaagtc aggtggacga agtgatttga 241 taccaactat caaatttcga cactgttctc tgttaagaaa tcGACGCTGC ACTGTAGCAT 301 ACCTGTATGA CCGCTTGCTT CGGATCAGAG CACTCAGATG GGAATATGGT AGCATCTTGC 361 CAAATGCATT ACGATTTCAC ATGGCTGCTG AAGAAATGGA GTGGTTTAAT AATTATAAAA 421 GATCTCTTGC TACTTATATG AGGTCACTGG GAGGAGATGA AGGTTTGGAC ATTACACAGG 481 ATATGAAACC ACCAAAAAGC CTATATATTG AAGTCCGGTG TCTAAAAGAC TATGGAGAAT 541 TTGAAGTTGA TGATGGCACT TCAGTCCTAT TAAAAAAAAA TAGCCAGCAC TTTTTACCTC 601 GATGGAAATG TGAGCAGCTG ATCAGACAAG GAGTCCTGGA GCACATCCTG TCATGCCCAA 661 CATTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 721 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 781 TATCTTGTGG AAAGGACGAA GCCATAGGTC TTTCACCCCA ACTACGCGTT AAGTCgacaa 841 tcaacctctg gattacaaaa tttgtgaaag att