Construct: ORF TRCN0000476126
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014773.1_s317c1
- Derived from:
- ccsbBroadEn_13918
- DNA Barcode:
- GGAGCGGGATGCTAGAAGAAGTCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- ENPP1 (5167)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476126
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5167 | ENPP1 | ectonucleotide pyrophosphat... | NM_006208.3 | 94.3% | 36.2% | 1_156del;1162G>T;2762T>G |
| 2 | mouse | 18605 | Enpp1 | ectonucleotide pyrophosphat... | NM_008813.4 | 78.4% | 32.5% | (many diffs) |
| 3 | mouse | 18605 | Enpp1 | ectonucleotide pyrophosphat... | NM_001308327.1 | 78.4% | 32.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1074
- ORF length:
- 1005
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggacgtgggg gaggagccgc tggagaaggc ggcgcgcgcc cgcactgcca 121 aggaccccaa cacctataaa gtactctcgc tggtattgtc agtatgtgtg ttaacaacaa 181 tacttggttg tatatttggg ttgaaaccaa gctgtgccaa agaagttaaa agttgcaaag 241 gtcgctgttt cgagagaaca tttgggaact gtcgctgtga tgctgcctgt gttgagcttg 301 gaaactgctg tttagattac caggagacgt gcatagaacc agaacatata tggacttgca 361 acaaattcag gtgtggtgag aaaaggttga ccagaagcct ctgtgcctgt tcagatgact 421 gcaaggacaa gggcgactgc tgcatcaact acagttctgt gtgtcaaggt gagaaaagtt 481 gggtagaaga accatgtgag agcattaatg agccacagtg cccagcaggg tttgaaacgc 541 ctcctaccct cttattttct ttggatggat tcagggcaga atatttacac acttggggtg 601 gacttcttcc tgttattagc aaactaaaaa aatgtggaac atatactaaa aacatgagac 661 cggtatatcc aacaaaaact ttccccaatc actacagcat tgtcaccgga ttgtatccag 721 aatctcatgg cataatcgac aataaaatgt atgatcccaa aatgaatgct tccttttcac 781 ttaaaagtaa agagaaattt aatcctgagt ggtacaaagg agaaccaatt tgggtcacag 841 ctaagtatca aggcctcaag tctggcacat ttttctggcc aggatcagat gtggaaatta 901 acggaatttt cccagacatc tataaaatgt ataatggttc agtaccattt gaagaaagga 961 ttttagctgt tcttcagtgg ctacagcttc ctaaagatga aagaccacac ttttacactc 1021 tgtatttaga agaaccagat tcttcaggtc attcatatgg accagtcagc agttaagtca 1081 tcaaagcctt gcagagggtt gatggtatgg ttggtatgct gatggatggt ctgaaagagc 1141 tgaacttgca cagatgcctg aacctcatcc ttatttcaga tcatggcatg gaacaaggca 1201 gttgtaagaa atacatatat ctgaataaat atttggggga tgttaaaaat attaaagtta 1261 tctatggacc tgcagctcga ttgagaccct ctgatgtccc agataaatac tattcattta 1321 actatgaagg cattgcccga aatctttctt gccgggaacc aaaccagcac ttcaaacctt 1381 acctgaaaca tttcttacct aagcgtttgc actttgctaa gagtgataga attgagccct 1441 tgacattcta tttggaccct cagtggcaac ttgcattgaa tccctcagaa aggaaatatt 1501 gtggaagtgg atttcatggc tctgacaatg tattttcaaa tatgcaagcc ctctttgttg 1561 gctatggacc tggattcaag catggcattg aggctgacac ctttgaaaac attgaagtct 1621 ataacttaat gtgtgattta ctgaatttga caccggctcc taataacgga actcatggaa 1681 gtcttaacca ccttctaaag aatcctgttt atacgccaaa gcatcccaaa gaagtgcacc 1741 ccctggtaca gtgccccttc acaagaaacc ccagagataa ccttggctgc tcatgtaacc 1801 cttcgatttt gccgattgag gattttcaaa cacagttcaa tctgactgtg gcagaagaga 1861 agattattaa gcatgaaact ttaccctatg gaagacctag agttctccag aaggaaaaca 1921 ccatctgtct tctttcccag caccagttta tgagtggata cagccaagac atcttaatgc 1981 ccctttggac atcctatacc gtggacagaa atgacagttt ctctacggaa gacttctcca 2041 actgtctgta ccaggacttt agaattcctc ttagtcctgt ccataaatgt tcattttata 2101 aaaataacac caaagtgagt tacgggttcc tctccccacc acaactaaat aaaaattcaa 2161 gtggaatata ttctgaagct ttgcttacta caaatatagt gccaatgtac cagagttttc 2221 aagttatatg gcgctacttt catgacaccc TACTGCGAAA GTATGCTGAA GAAAGAAATG 2281 GTGTCAATGT CGTCAGTGGT CCTGTGTTTG ACTTTGATTA TGATGGACGT TGTGATTCCT 2341 TAGAGAATCT GAGGCAAAAA AGAAGAGTCA TCCGTAACCA AGAAATTTTG ATTCCAACTC 2401 ACTTCTTTAT TGTGCTAACA AGCTGTAAAG ATACATCTCA GACGCCTTTG CACTGTGAAA 2461 ACCTAGACAC CTTAGCTTTC ATTTTGCCTC ACAGGACTGA TAACAGCGAG AGCTGTGTGC 2521 ATGGGAAGCA TGACTCCTCA TGGGTTGAAG AATTGTTAAT GTTACACAGA GCACGGATCA 2581 CAGATGTTGA GCACATCACT GGACTCAGCT TCTATCAACA AAGAAAAGAG CCAGTTTCAG 2641 ACATTTTAAA GTTGAAAACA CATTTGCCAA CCTGTAGCCA AGAAGACTTG CCAACTTTCT 2701 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 2761 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 2821 GTGGAAAGGA CGAGGAGCGG GATGCTAGAA GAAGTCCACG CGTTAAGTCg acaatcaacc 2881 tctggattac aaaatttgtg aaagatt